ID   CAPB_BACAN              Reviewed;         464 AA.
AC   P19580; Q9RMX5;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   18-OCT-2001, sequence version 2.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=Capsule biosynthesis protein CapB;
GN   Name=capB; OrderedLocusNames=pXO2-58, BXB0066, GBAA_pXO2_0066;
OS   Bacillus anthracis.
OG   Plasmid pXO2, and Plasmid pTE702.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1392;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Pasteur; PLASMID=pXO2;
RX   PubMed=10475962; DOI=10.1046/j.1365-2672.1999.00883.x;
RA   Okinaka R.T., Cloud K., Hampton O., Hoffmaster A., Hill K.K., Keim P.,
RA   Koehler T., Lamke G., Kumano S., Manter D., Martinez Y., Ricke D.,
RA   Svensson R., Jackson P.J.;
RT   "Sequence, assembly and analysis of pXO1 and pXO2.";
RL   J. Appl. Microbiol. 87:261-262(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Ames / isolate Florida / A2012; PLASMID=pXO2;
RX   PubMed=12004073; DOI=10.1126/science.1071837;
RA   Read T.D., Salzberg S.L., Pop M., Shumway M.F., Umayam L., Jiang L.,
RA   Holtzapple E., Busch J.D., Smith K.L., Schupp J.M., Solomon D., Keim P.,
RA   Fraser C.M.;
RT   "Comparative genome sequencing for discovery of novel polymorphisms in
RT   Bacillus anthracis.";
RL   Science 296:2028-2033(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames ancestor; PLASMID=pXO2;
RX   PubMed=18952800; DOI=10.1128/jb.01347-08;
RA   Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA   Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT   "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL   J. Bacteriol. 191:445-446(2009).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 68-464.
RC   PLASMID=pTE702;
RX   PubMed=2536679; DOI=10.1128/jb.171.2.722-730.1989;
RA   Makino S., Uchida I., Terakado N., Sasakawa C., Yoshikawa M.;
RT   "Molecular characterization and protein analysis of the cap region, which
RT   is essential for encapsulation in Bacillus anthracis.";
RL   J. Bacteriol. 171:722-730(1989).
RN   [5]
RP   INDUCTION.
RC   PLASMID=pXO2;
RX   PubMed=14702298; DOI=10.1128/jb.186.2.307-315.2004;
RA   Drysdale M., Bourgogne A., Hilsenbeck S.G., Koehler T.M.;
RT   "atxA controls Bacillus anthracis capsule synthesis via acpA and a newly
RT   discovered regulator, acpB.";
RL   J. Bacteriol. 186:307-315(2004).
CC   -!- FUNCTION: Essential for the synthesis of the polyglutamate capsule of
CC       B.anthracis which is one of the principal virulence factors during
CC       anthrax infection. May form a polyglutamyl synthetase complex together
CC       with proteins CapA and CapC.
CC   -!- PATHWAY: Capsule biogenesis; capsule polysaccharide biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Single-pass membrane protein.
CC   -!- INDUCTION: Capsule synthesis is transcriptionally regulated by AtxA,
CC       AcpA and AcpB. {ECO:0000269|PubMed:14702298}.
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DR   EMBL; AF188935; AAF13663.1; -; Genomic_DNA.
DR   EMBL; AE011191; AAM26222.1; -; Genomic_DNA.
DR   EMBL; AE017335; AAT28996.2; -; Genomic_DNA.
DR   EMBL; M24150; AAA22286.1; -; Genomic_DNA.
DR   PIR; A30091; A30091.
DR   RefSeq; NP_053213.1; NC_002146.1.
DR   RefSeq; WP_000576220.1; NZ_QAEN01000011.1.
DR   RefSeq; WP_003159943.1; NZ_VTZL01000009.1.
DR   AlphaFoldDB; P19580; -.
DR   SMR; P19580; -.
DR   EnsemblBacteria; AAT28996; AAT28996; GBAA_pXO2_0066.
DR   GeneID; 39682772; -.
DR   GeneID; 45025367; -.
DR   KEGG; bar:GBAA_pXO2_0066; -.
DR   HOGENOM; CLU_041144_0_0_9; -.
DR   OMA; NECMAVN; -.
DR   BioCyc; MetaCyc:MON-16980; -.
DR   UniPathway; UPA00934; -.
DR   Proteomes; UP000000594; Plasmid pXO2.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0016879; F:ligase activity, forming carbon-nitrogen bonds; IEA:UniProt.
DR   GO; GO:0045227; P:capsule polysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.1190.10; -; 1.
DR   InterPro; IPR008337; Capsule_biosynth_CapB.
DR   InterPro; IPR036565; Mur-like_cat_sf.
DR   InterPro; IPR013221; Mur_ligase_cen.
DR   Pfam; PF08245; Mur_ligase_M; 1.
DR   PRINTS; PR01758; CAPSULEPROTB.
DR   SUPFAM; SSF53623; SSF53623; 1.
DR   TIGRFAMs; TIGR04012; poly_gGlu_PgsB; 1.
PE   2: Evidence at transcript level;
KW   Capsule biogenesis/degradation; Cell membrane; Membrane; Plasmid;
KW   Reference proteome; Transmembrane; Transmembrane helix; Virulence.
FT   CHAIN           1..464
FT                   /note="Capsule biosynthesis protein CapB"
FT                   /id="PRO_0000089309"
FT   TRANSMEM        65..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   464 AA;  52901 MW;  E206A103ED53C52B CRC64;
     MKNIKIVRIL KHDEAIRIEH RISELYSDEF GVVYAGNHLI FNWYQRLYLS RNILISKKSK
     SRKGLIQMIF IIGICTVFLI IYGIWEQRCH QKRLNSIPIR VNINGIRGKS TVTRLITGVV
     QEAKYKTVGK TTGTSARMIY WFTDEEQPIK RRKEGPNIGE QRRVVKEAAD LEAEALICEC
     MAVQPDYQII FQNKMIQANV GVIVNVLEDH MDVMGPTLDE VAEAFTATIP YNGHLVTIES
     EYLDYFKEVA EERNTKVIVA DNSRISEEFL RKFDYMVFPD NASLALAVAE ALGIDEETAF
     RGMLNAHPDP GAMRITRFAD QSKPAFFVNG FAANDPSSTL RIWERVDDFG YSNLAPIVIM
     NCRPDRVDRT EQFARDVLPY IKAEIVIAIG ETTAPITSAF EKGDIPTQEY WNLEGWSTSE
     IMSRMRPYLK NRIVYGVGNI HGAAEPLIDM IMEEQIGKKQ AKVI