ID   CAPB_BACSU              Reviewed;         393 AA.
AC   P96736; P96735; Q798J2; Q798J3; Q9AJM3; Q9FAF4; Q9R9J3;
DT   07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 3.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=PGA synthase CapB;
DE            EC=6.3.2.-;
DE   AltName: Full=Gamma-polyglutamic acid synthase;
GN   Name=capB; Synonyms=pgsB, ywsC; OrderedLocusNames=BSU35900;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9353933; DOI=10.1099/00221287-143-10-3313;
RA   Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V.,
RA   Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G.,
RA   Kunst F., Danchin A., Glaser P.;
RT   "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334
RT   degrees).";
RL   Microbiology 143:3313-3328(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NBRC 3336;
RX   PubMed=10486244; DOI=10.1006/bbrc.1999.1298;
RA   Ashiuchi M., Soda K., Misono H.;
RT   "A poly-gamma-glutamate synthetic system of Bacillus subtilis IFO 3336:
RT   gene cloning and biochemical analysis of poly-gammma-glutamate produced by
RT   Escherichia coli clone cells.";
RL   Biochem. Biophys. Res. Commun. 263:6-12(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Asahikawa;
RA   Tran L.P., Itoh Y.;
RT   "Nucleotide sequence of the capBCA operon in Bacillus subtilis (natto).";
RL   Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, AND
RP   ALTERNATIVE INITIATION.
RC   STRAIN=NBRC 16449;
RX   PubMed=11751809; DOI=10.1128/jb.184.2.337-343.2002;
RA   Urushibata Y., Tokuyama S., Tahara Y.;
RT   "Characterization of the Bacillus subtilis ywsC gene, involved in gamma-
RT   polyglutamic acid production.";
RL   J. Bacteriol. 184:337-343(2002).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [6]
RP   SEQUENCE REVISION TO 360.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
CC   -!- FUNCTION: Catalyzes the biosynthesis of PGA (gamma-polyglutamic acid)
CC       from L-glutamate. Both the 44-kDa and the 33-kDa forms are required for
CC       PGA synthesis. {ECO:0000269|PubMed:11751809}.
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=44-kDa;
CC         IsoId=P96736-1; Sequence=Displayed;
CC       Name=33-kDa;
CC         IsoId=P96736-2; Sequence=VSP_018777;
CC   -!- DISRUPTION PHENOTYPE: Cells do not produce PGA.
CC       {ECO:0000269|PubMed:11751809}.
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DR   EMBL; Z92953; CAB07466.1; -; Genomic_DNA.
DR   EMBL; Z92954; CAB07475.1; -; Genomic_DNA.
DR   EMBL; AB016245; BAA85263.1; -; Genomic_DNA.
DR   EMBL; AB039950; BAB13484.1; -; Genomic_DNA.
DR   EMBL; AB046355; BAB40948.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15607.2; -; Genomic_DNA.
DR   PIR; F70069; F70069.
DR   RefSeq; NP_391471.2; NC_000964.3. [P96736-1]
DR   RefSeq; WP_003227883.1; NZ_JNCM01000034.1.
DR   AlphaFoldDB; P96736; -.
DR   SMR; P96736; -.
DR   STRING; 224308.BSU35900; -.
DR   PaxDb; P96736; -.
DR   PRIDE; P96736; -.
DR   EnsemblBacteria; CAB15607; CAB15607; BSU_35900.
DR   GeneID; 936833; -.
DR   KEGG; bsu:BSU35900; -.
DR   PATRIC; fig|224308.179.peg.3886; -.
DR   eggNOG; COG0771; Bacteria.
DR   InParanoid; P96736; -.
DR   OMA; NECMAVN; -.
DR   BioCyc; BSUB:BSU35900-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0016879; F:ligase activity, forming carbon-nitrogen bonds; IEA:UniProt.
DR   GO; GO:0045227; P:capsule polysaccharide biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.1190.10; -; 1.
DR   InterPro; IPR008337; Capsule_biosynth_CapB.
DR   InterPro; IPR036565; Mur-like_cat_sf.
DR   InterPro; IPR013221; Mur_ligase_cen.
DR   Pfam; PF08245; Mur_ligase_M; 1.
DR   PRINTS; PR01758; CAPSULEPROTB.
DR   SUPFAM; SSF53623; SSF53623; 1.
DR   TIGRFAMs; TIGR04012; poly_gGlu_PgsB; 1.
PE   4: Predicted;
KW   Alternative initiation; Ligase; Reference proteome.
FT   CHAIN           1..393
FT                   /note="PGA synthase CapB"
FT                   /id="PRO_0000020842"
FT   VAR_SEQ         96
FT                   /note="Missing (in isoform 33-kDa)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_018777"
FT   VARIANT         89
FT                   /note="N -> S (in strain: IFO 3336)"
FT   VARIANT         139
FT                   /note="L -> F (in strain: Asahikawa)"
FT   VARIANT         324..325
FT                   /note="TT -> QQ (in strain: IFO 16449)"
FT   VARIANT         360
FT                   /note="R -> S (in strain: IFO 3336)"
FT   CONFLICT        360
FT                   /note="R -> S (in Ref. 2; CAB07475)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   393 AA;  44017 MW;  605A5DA1305BDA08 CRC64;
     MWLLIIACAV ILVIGILEKR RHQKNIDALP VRVNINGIRG KSTVTRLTTG ILIEAGYKTV
     GKTTGTDARM IYWDTPEEKP IKRKPQGPNI GEQKEVMRET VERGANAIVS ECMAVNPDYQ
     IIFQEELLQA NIGVIVNVLE DHMDVMGPTL DEIAEAFTAT IPYNGHLVIT DSEYTEFFKQ
     KAKERNTKVI IADNSKITDE YLRKFEYMVF PDNASLALGV AQALGIDEET AFKGMLNAPP
     DPGAMRILPL ISPSEPGHFV NGFAANDASS TLNIWKRVKE IGYPTDDPII IMNCRADRVD
     RTQQFANDVL PYIEASELIL IGETTEPIVK AYEEGKIPAD KLHDLEYKST DEIMELLKKR
     MHNRVIYGVG NIHGAAEPLI EKIHEYKVKQ LVS