ID   XYLC_PSEPU              Reviewed;         487 AA.
AC   P43503;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Benzaldehyde dehydrogenase [NAD(+)];
DE            EC=1.2.1.28;
GN   Name=xylC;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OG   Plasmid TOL pWW0, and Plasmid TOL pWW53.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-15.
RC   PLASMID=TOL pWW0;
RX   PubMed=7868591; DOI=10.1128/jb.177.5.1196-1201.1995;
RA   Inoue J., Shaw J.P., Rekik M., Harayama S.;
RT   "Overlapping substrate specificities of benzaldehyde dehydrogenase (the
RT   xylC gene product) and 2-hydroxymuconic semialdehyde dehydrogenase (the
RT   xylG gene product) encoded by TOL plasmid pWW0 of Pseudomonas putida.";
RL   J. Bacteriol. 177:1196-1201(1995).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-53.
RC   PLASMID=TOL pWW53;
RX   PubMed=1989592; DOI=10.1042/bj2730099;
RA   Chalmers R.M., Keen J.N., Fewson C.A.;
RT   "Comparison of benzyl alcohol dehydrogenases and benzaldehyde
RT   dehydrogenases from the benzyl alcohol and mandelate pathways in
RT   Acinetobacter calcoaceticus and from the TOL-plasmid-encoded toluene
RT   pathway in Pseudomonas putida. N-terminal amino acid sequences, amino acid
RT   compositions and immunological cross-reactions.";
RL   Biochem. J. 273:99-107(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=benzaldehyde + H2O + NAD(+) = benzoate + 2 H(+) + NADH;
CC         Xref=Rhea:RHEA:11840, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16150, ChEBI:CHEBI:17169, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.2.1.28;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 9.0.;
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; U15151; AAA66218.1; -; Genomic_DNA.
DR   EMBL; D63341; BAA09661.1; -; Genomic_DNA.
DR   PIR; T47107; T47107.
DR   RefSeq; NP_542888.1; NC_003350.1.
DR   RefSeq; WP_011005931.1; NZ_QWEF01000005.1.
DR   AlphaFoldDB; P43503; -.
DR   SMR; P43503; -.
DR   KEGG; ag:AAA66218; -.
DR   BioCyc; MetaCyc:MON-2968; -.
DR   GO; GO:0018479; F:benzaldehyde dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0071704; P:organic substance metabolic process; IEA:UniProt.
DR   Gene3D; 3.40.309.10; -; 1.
DR   Gene3D; 3.40.605.10; -; 1.
DR   InterPro; IPR016161; Ald_DH/histidinol_DH.
DR   InterPro; IPR016163; Ald_DH_C.
DR   InterPro; IPR016160; Ald_DH_CS_CYS.
DR   InterPro; IPR029510; Ald_DH_CS_GLU.
DR   InterPro; IPR016162; Ald_DH_N.
DR   InterPro; IPR015590; Aldehyde_DH_dom.
DR   Pfam; PF00171; Aldedh; 1.
DR   SUPFAM; SSF53720; SSF53720; 1.
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Direct protein sequencing; NAD;
KW   Oxidoreductase; Plasmid.
FT   CHAIN           1..487
FT                   /note="Benzaldehyde dehydrogenase [NAD(+)]"
FT                   /id="PRO_0000056591"
FT   ACT_SITE        254
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        288
FT                   /evidence="ECO:0000250"
FT   BINDING         232..237
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   487 AA;  51897 MW;  093CB3E9487AF384 CRC64;
     MRETKEQPIW YGKVFSSNWV EARGGVANVV DPSNGDILGI TGVANGEDVD AAVNAAKRAQ
     KEWAAIPFSE RAAIVRKAAE KLKEREYEFA DWNVRECGAI RPKGLWEAGI AYEQMHQAAG
     LASLPNGTLF PSAVPGRMNL CQRVPVGVVG VIAPWNFPLF LAMRSVAPAL ALGNAVILKP
     DLQTAVTGGA LIAEIFSDAG MPDGVLHVLP GGADVGESMV ANSGINMISF TGSTQVGRLI
     GEKCGRMLKK VALELGGNNV HIVLPDADLE GAVSCAAWGT FLHQGQVCMA AGRHLVHRDV
     AQQYAEKLAL RAKNLVVGDP NSDQVHLGPL INEKQVVRVH ALVESAQRAG AQVLAGGTYQ
     DRYYQATVIM DVKPEMEVFK SEIFGPVAPI TVFDSIEEAI ELANCSEYGL AASIHTRALA
     TGLDIAKRLN TGMVHINDQP INCEPHVPFG GMGASGSGGR FGGPASIEEF TQSQWISMVE
     KPANYPF