ID   XYLD_AGRFC              Reviewed;         350 AA.
AC   Q8U7Y1;
DT   19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Putative D-xylulose reductase;
DE            EC=1.1.1.9;
DE   AltName: Full=Xylitol dehydrogenase;
DE            Short=XDH;
GN   OrderedLocusNames=Atu4318; ORFNames=AGR_L_1091;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + xylitol = D-xylulose + H(+) + NADH;
CC         Xref=Rhea:RHEA:20433, ChEBI:CHEBI:15378, ChEBI:CHEBI:17140,
CC         ChEBI:CHEBI:17151, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.9;
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC       family. {ECO:0000305}.
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DR   EMBL; AE007870; AAK89121.1; -; Genomic_DNA.
DR   PIR; AB3087; AB3087.
DR   PIR; G98199; G98199.
DR   RefSeq; NP_356336.1; NC_003063.2.
DR   RefSeq; WP_010973745.1; NC_003063.2.
DR   AlphaFoldDB; Q8U7Y1; -.
DR   SMR; Q8U7Y1; -.
DR   STRING; 176299.Atu4318; -.
DR   EnsemblBacteria; AAK89121; AAK89121; Atu4318.
DR   GeneID; 66224547; -.
DR   KEGG; atu:Atu4318; -.
DR   PATRIC; fig|176299.10.peg.4128; -.
DR   eggNOG; COG1063; Bacteria.
DR   HOGENOM; CLU_026673_11_5_5; -.
DR   OMA; QFGVADY; -.
DR   PhylomeDB; Q8U7Y1; -.
DR   BioCyc; AGRO:ATU4318-MON; -.
DR   Proteomes; UP000000813; Chromosome linear.
DR   GO; GO:0046526; F:D-xylulose reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd05285; sorbitol_DH; 1.
DR   InterPro; IPR013149; ADH-like_C.
DR   InterPro; IPR013154; ADH_N.
DR   InterPro; IPR002328; ADH_Zn_CS.
DR   InterPro; IPR011032; GroES-like_sf.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020843; PKS_ER.
DR   InterPro; IPR045306; SDH-like.
DR   Pfam; PF08240; ADH_N; 1.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   SMART; SM00829; PKS_ER; 1.
DR   SUPFAM; SSF50129; SSF50129; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00059; ADH_ZINC; 1.
PE   3: Inferred from homology;
KW   Metal-binding; NAD; Oxidoreductase; Reference proteome; Zinc.
FT   CHAIN           1..350
FT                   /note="Putative D-xylulose reductase"
FT                   /id="PRO_0000160884"
FT   BINDING         43
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         68
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
FT   BINDING         154
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   350 AA;  36776 MW;  9C6FB260277F137E CRC64;
     MKALVLEEKG KLSLRDFDIP GGAGSGELGP KDVRIRTHTV GICGSDVHYY THGKIGHFVV
     NAPMVLGHEA SGTVIETGSD VTHLKIGDRV CMEPGIPDPT SRASKLGIYN VDPAVRFWAT
     PPIHGCLTPE VIHPAAFTYK LPDNVSFAEG AMVEPFAIGM QAALRARIQP GDIAVVTGAG
     PIGMMVALAA LAGGCAKVIV ADLAQPKLDI IAAYDGIETI NIRERNLAEA VSAATDGWGC
     DIVFECSGAA PAILGMAKLA RPGGAIVLVG MPVDPVPVDI VGLQAKELRV ETVFRYANVY
     DRAVALIASG KVDLKPLISA TIPFEDSIAG FDRAVEARET DVKLQIVMPQ