XYLF_ECOLI
ID XYLF_ECOLI Reviewed; 330 AA.
AC P37387; Q2M7M9;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=D-xylose-binding periplasmic protein;
DE Flags: Precursor;
GN Name=xylF; Synonyms=xylT; OrderedLocusNames=b3566, JW3538;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 24-67.
RA Sumiya M., Henderson P.J.F.;
RT "The D-xylose binding protein of Escherichia coli.";
RL Biochem. Soc. Trans. 17:553-554(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP PROTEIN SEQUENCE OF 24-71.
RX PubMed=8581399;
RA Sumiya M., Davis E.O., Packman L.C., McDonald T.P., Henderson P.J.;
RT "Molecular genetics of a receptor protein for D-xylose, encoded by the gene
RT xylF, in Escherichia coli.";
RL Recept. Channels 3:117-128(1995).
RN [6]
RP PROTEIN SEQUENCE OF 24-35.
RC STRAIN=K12 / EMG2;
RX PubMed=9298646; DOI=10.1002/elps.1150180807;
RA Link A.J., Robison K., Church G.M.;
RT "Comparing the predicted and observed properties of proteins encoded in the
RT genome of Escherichia coli K-12.";
RL Electrophoresis 18:1259-1313(1997).
RN [7]
RP IDENTIFICATION BY 2D-GEL.
RX PubMed=9298644; DOI=10.1002/elps.1150180805;
RA VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT "Escherichia coli proteome analysis using the gene-protein database.";
RL Electrophoresis 18:1243-1251(1997).
CC -!- FUNCTION: Involved in the high-affinity D-xylose membrane transport
CC system. Binds with high affinity to xylose.
CC -!- SUBCELLULAR LOCATION: Periplasm.
CC -!- MISCELLANEOUS: E.coli has two D-xylose transport systems that
CC accumulate sugar against a concentration gradient: the XylE system
CC which utilizes the electrochemical gradient of protons and that is
CC insensitive to cold osmotic shock and the XylF system that uses a high-
CC energy phosphate compound and is sensitive to cold osmotic shock.
CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 2 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U00039; AAB18543.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76590.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77727.1; -; Genomic_DNA.
DR PIR; S47787; S47787.
DR RefSeq; NP_418023.1; NC_000913.3.
DR RefSeq; WP_000694881.1; NZ_SSZK01000041.1.
DR PDB; 3M9W; X-ray; 2.15 A; A=24-330.
DR PDB; 3M9X; X-ray; 2.20 A; A=24-330.
DR PDB; 3MA0; X-ray; 2.20 A; A/B/C=24-330.
DR PDBsum; 3M9W; -.
DR PDBsum; 3M9X; -.
DR PDBsum; 3MA0; -.
DR AlphaFoldDB; P37387; -.
DR SMR; P37387; -.
DR BioGRID; 4262541; 29.
DR BioGRID; 852398; 1.
DR ComplexPortal; CPX-4388; Xylose ABC transporter complex.
DR IntAct; P37387; 4.
DR STRING; 511145.b3566; -.
DR TCDB; 3.A.1.2.4; the atp-binding cassette (abc) superfamily.
DR jPOST; P37387; -.
DR PaxDb; P37387; -.
DR PRIDE; P37387; -.
DR EnsemblBacteria; AAC76590; AAC76590; b3566.
DR EnsemblBacteria; BAE77727; BAE77727; BAE77727.
DR GeneID; 67417211; -.
DR GeneID; 948090; -.
DR KEGG; ecj:JW3538; -.
DR KEGG; eco:b3566; -.
DR PATRIC; fig|1411691.4.peg.3146; -.
DR EchoBASE; EB4153; -.
DR eggNOG; COG4213; Bacteria.
DR HOGENOM; CLU_037628_13_0_6; -.
DR InParanoid; P37387; -.
DR OMA; YTDGWLP; -.
DR PhylomeDB; P37387; -.
DR BioCyc; EcoCyc:XYLF-MON; -.
DR BioCyc; MetaCyc:XYLF-MON; -.
DR EvolutionaryTrace; P37387; -.
DR PRO; PR:P37387; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IC:ComplexPortal.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IDA:EcoCyc.
DR GO; GO:0030246; F:carbohydrate binding; IBA:GO_Central.
DR GO; GO:0048029; F:monosaccharide binding; IMP:EcoCyc.
DR GO; GO:0042732; P:D-xylose metabolic process; IMP:EcoCyc.
DR GO; GO:0015753; P:D-xylose transmembrane transport; IMP:EcoCyc.
DR InterPro; IPR028082; Peripla_BP_I.
DR InterPro; IPR025997; SBP_2_dom.
DR InterPro; IPR013456; XylF.
DR Pfam; PF13407; Peripla_BP_4; 1.
DR SUPFAM; SSF53822; SSF53822; 1.
DR TIGRFAMs; TIGR02634; xylF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Periplasm; Reference proteome;
KW Signal; Sugar transport; Transport.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:8581399,
FT ECO:0000269|PubMed:9298646, ECO:0000269|Ref.1"
FT CHAIN 24..330
FT /note="D-xylose-binding periplasmic protein"
FT /id="PRO_0000031738"
FT STRAND 27..33
FT /evidence="ECO:0007829|PDB:3M9W"
FT STRAND 36..38
FT /evidence="ECO:0007829|PDB:3M9W"
FT HELIX 41..53
FT /evidence="ECO:0007829|PDB:3M9W"
FT STRAND 57..62
FT /evidence="ECO:0007829|PDB:3M9W"
FT HELIX 67..79
FT /evidence="ECO:0007829|PDB:3M9W"
FT STRAND 83..88
FT /evidence="ECO:0007829|PDB:3M9W"
FT HELIX 96..103
FT /evidence="ECO:0007829|PDB:3M9W"
FT TURN 104..106
FT /evidence="ECO:0007829|PDB:3M9W"
FT STRAND 108..114
FT /evidence="ECO:0007829|PDB:3M9W"
FT STRAND 122..127
FT /evidence="ECO:0007829|PDB:3M9W"
FT HELIX 129..143
FT /evidence="ECO:0007829|PDB:3M9W"
FT STRAND 145..154
FT /evidence="ECO:0007829|PDB:3M9W"
FT HELIX 159..177
FT /evidence="ECO:0007829|PDB:3M9W"
FT STRAND 180..188
FT /evidence="ECO:0007829|PDB:3M9W"
FT HELIX 190..192
FT /evidence="ECO:0007829|PDB:3M9W"
FT HELIX 194..207
FT /evidence="ECO:0007829|PDB:3M9W"
FT TURN 208..210
FT /evidence="ECO:0007829|PDB:3M9W"
FT STRAND 214..219
FT /evidence="ECO:0007829|PDB:3M9W"
FT HELIX 220..231
FT /evidence="ECO:0007829|PDB:3M9W"
FT TURN 232..234
FT /evidence="ECO:0007829|PDB:3M9W"
FT TURN 236..238
FT /evidence="ECO:0007829|PDB:3M9W"
FT STRAND 239..241
FT /evidence="ECO:0007829|PDB:3M9W"
FT HELIX 248..256
FT /evidence="ECO:0007829|PDB:3M9W"
FT STRAND 262..264
FT /evidence="ECO:0007829|PDB:3M9W"
FT HELIX 267..282
FT /evidence="ECO:0007829|PDB:3M9W"
FT STRAND 290..294
FT /evidence="ECO:0007829|PDB:3M9W"
FT STRAND 296..305
FT /evidence="ECO:0007829|PDB:3M9W"
FT STRAND 308..310
FT /evidence="ECO:0007829|PDB:3M9W"
FT TURN 312..315
FT /evidence="ECO:0007829|PDB:3M9W"
FT HELIX 316..320
FT /evidence="ECO:0007829|PDB:3M9W"
FT TURN 321..323
FT /evidence="ECO:0007829|PDB:3M9W"
FT HELIX 327..329
FT /evidence="ECO:0007829|PDB:3M9W"
SQ SEQUENCE 330 AA; 35734 MW; E0AFC0326511B056 CRC64;
MKIKNILLTL CTSLLLTNVA AHAKEVKIGM AIDDLRLERW QKDRDIFVKK AESLGAKVFV
QSANGNEETQ MSQIENMINR GVDVLVIIPY NGQVLSNVVK EAKQEGIKVL AYDRMINDAD
IDFYISFDNE KVGELQAKAL VDIVPQGNYF LMGGSPVDNN AKLFRAGQMK VLKPYVDSGK
IKVVGDQWVD GWLPENALKI MENALTANNN KIDAVVASND ATAGGAIQAL SAQGLSGKVA
ISGQDADLAG IKRIAAGTQT MTVYKPITLL ANTAAEIAVE LGNGQEPKAD TTLNNGLKDV
PSRLLTPIDV NKNNIKDTVI KDGFHKESEL
