ID   XYLF_PSEPU              Reviewed;         281 AA.
AC   P23106;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=2-hydroxymuconate semialdehyde hydrolase;
DE            Short=HMSH;
DE            EC=3.7.1.9;
DE   AltName: Full=2-hydroxymuconic semialdehyde hydrolase;
GN   Name=xylF;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OG   Plasmid TOL pWW0.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-23.
RX   PubMed=1791759; DOI=10.1111/j.1365-2958.1991.tb02091.x;
RA   Horn J.M., Harayama S., Timmis K.N.;
RT   "DNA sequence determination of the TOL plasmid (pWWO) xylGFJ genes of
RT   Pseudomonas putida: implications for the evolution of aromatic
RT   catabolism.";
RL   Mol. Microbiol. 5:2459-2474(1991).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   DOI=10.1099/00221287-132-3-717;
RA   Duggleby C.J., Williams P.A.;
RT   "Purification and some properties of the 2-hydroxy-6-oxohepta-2,4-dienoate
RT   hydrolase (2-hydroxymuconic semialdehyde hydrolase) encoded by the TOL
RT   plasmid pWW0 from Pseudomonas putida mt-2.";
RL   J. Gen. Microbiol. 132:2459-2474(1991).
CC   -!- FUNCTION: Catalyzes the conversion of 2-hydroxymuconate semialdehyde to
CC       2-hydroxypent-2,4-dienoate. {ECO:0000269|Ref.2}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H2O = 2-oxopent-4-
CC         enoate + formate + H(+); Xref=Rhea:RHEA:14549, ChEBI:CHEBI:11641,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740,
CC         ChEBI:CHEBI:71198; EC=3.7.1.9; Evidence={ECO:0000269|Ref.2};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=30 uM for catechol {ECO:0000269|Ref.2};
CC         KM=36 uM for 3-methylcatechol {ECO:0000269|Ref.2};
CC         KM=10 uM for 4-methylcatechol {ECO:0000269|Ref.2};
CC         KM=15 uM for 3-ethylcatechol {ECO:0000269|Ref.2};
CC         KM=22 uM for 4-ethylcatechol {ECO:0000269|Ref.2};
CC         KM=5 uM for 3-propylcatechol {ECO:0000269|Ref.2};
CC         KM=23 uM for 4-propylcatechol {ECO:0000269|Ref.2};
CC         KM=37 uM for 3-allylcatechol {ECO:0000269|Ref.2};
CC       pH dependence:
CC         Optimum pH is 7-7.5. {ECO:0000269|Ref.2};
CC   -!- PATHWAY: Aromatic compound metabolism; benzoate degradation via
CC       hydroxylation.
CC   -!- SIMILARITY: Belongs to the DmpD/TodF/XylF esterase family.
CC       {ECO:0000305}.
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DR   EMBL; M64747; AAA26054.1; -; Genomic_DNA.
DR   PIR; S18245; S18245.
DR   RefSeq; NP_542864.1; NC_003350.1.
DR   RefSeq; WP_011005907.1; NC_003350.1.
DR   AlphaFoldDB; P23106; -.
DR   SMR; P23106; -.
DR   ESTHER; psepu-XYLF; Carbon-carbon_bond_hydrolase.
DR   BioCyc; MetaCyc:MON-12763; -.
DR   SABIO-RK; P23106; -.
DR   UniPathway; UPA00156; -.
DR   GO; GO:0018775; F:2-hydroxymuconate-semialdehyde hydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0043640; P:benzoate catabolic process via hydroxylation; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   PRINTS; PR00111; ABHYDROLASE.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Direct protein sequencing; Hydrolase;
KW   Plasmid; Serine esterase.
FT   CHAIN           1..281
FT                   /note="2-hydroxymuconate semialdehyde hydrolase"
FT                   /id="PRO_0000207058"
FT   DOMAIN          31..261
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   REGION          30..55
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        106
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        227
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        255
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   281 AA;  30598 MW;  ED8D215E6DD47DF5 CRC64;
     MNAPQQSPEI GREILAAGYR TNLHDQGEGF PALLIHGSGP ASPPGPTGAG SFRSSQTRRV
     IAPDMLGFGY SERPADGKYS QARWVEHAIG VLDALGIQQG DIVGNSFGGG LALALAIRHP
     ERVRRLVLMG SVGVSFPITA GLETAWGYTP SLANMRRLLD LFAHDRTLVN DELAELRYQA
     SIRPGFQESF AAMFPPPRQN GVDDLASNET DIRALPNETL VIHGREDRII PLQASLTLAQ
     WIPNAQLHVF GQCGHWTQIE HAERFARLVE NFLAEADALH S