ID   XYLJ_PSEPU              Reviewed;         222 AA.
AC   P23107;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=2-hydroxypent-2,4-dienoate hydratase;
DE            Short=HPH;
DE            EC=4.2.-.-;
DE   AltName: Full=2-oxopent-4-enoate hydratase;
GN   Name=xylJ;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OG   Plasmid TOL pWW0.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-22.
RX   PubMed=1791759; DOI=10.1111/j.1365-2958.1991.tb02091.x;
RA   Horn J.M., Harayama S., Timmis K.N.;
RT   "DNA sequence determination of the TOL plasmid (pWWO) xylGFJ genes of
RT   Pseudomonas putida: implications for the evolution of aromatic
RT   catabolism.";
RL   Mol. Microbiol. 5:2459-2474(1991).
CC   -!- FUNCTION: Conversion of 2-hydroxypent-2,4-dienoate into 4-hydroxy-2-
CC       oxopentanoate.
CC   -!- PATHWAY: Aromatic compound metabolism; benzoate degradation via
CC       hydroxylation.
CC   -!- SIMILARITY: Belongs to the hydratase/decarboxylase family.
CC       {ECO:0000305}.
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DR   EMBL; M64747; AAA26055.1; -; Genomic_DNA.
DR   PIR; S18246; S18246.
DR   RefSeq; NP_542863.1; NC_003350.1.
DR   RefSeq; WP_011005906.1; NC_003350.1.
DR   AlphaFoldDB; P23107; -.
DR   SMR; P23107; -.
DR   BioCyc; MetaCyc:MON-3403; -.
DR   UniPathway; UPA00156; -.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0043640; P:benzoate catabolic process via hydroxylation; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.850.10; -; 1.
DR   InterPro; IPR036663; Fumarylacetoacetase_C_sf.
DR   SUPFAM; SSF56529; SSF56529; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Direct protein sequencing; Lyase;
KW   Plasmid.
FT   CHAIN           1..222
FT                   /note="2-hydroxypent-2,4-dienoate hydratase"
FT                   /id="PRO_0000066055"
SQ   SEQUENCE   222 AA;  23883 MW;  D6BAB8DB06EDC1B9 CRC64;
     MDKTLINELG DELYQAMVQR ETVTPLTSRG FDISVEDAYH ISLRMLERRL AAGERVIGKK
     IGVTSKAVQN MLGVHQPDFG YLTDAMVYNS GEAMPISEKL IQPRAEGEIA FILKKDLMGP
     GVTNADVLAA TECVIPCFEV VDSRIQDWKI KIQDTVADNA SCGLFVLGDQ AVSPRQVDLV
     TCGMLVEKNG QLLSTGAGAA ALGSPVNCVA WLANTLGHFG IA