ID   XYLR_PSEPU              Reviewed;         566 AA.
AC   P06519;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Transcriptional regulatory protein XylR;
DE   AltName: Full=67 kDa protein;
GN   Name=xylR;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OG   Plasmid TOL pWW0.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3169574; DOI=10.1016/0378-1119(88)90366-6;
RA   Inouye S., Nakazawa A., Nakazawa T.;
RT   "Nucleotide sequence of the regulatory gene xylR of the TOL plasmid from
RT   Pseudomonas putida.";
RL   Gene 66:301-306(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.
RX   PubMed=2993247; DOI=10.1128/jb.163.3.863-869.1985;
RA   Inouye S., Nakazawa A., Nakazawa T.;
RT   "Determination of the transcription initiation site and identification of
RT   the protein product of the regulatory gene xylR for xyl operons on the TOL
RT   plasmid.";
RL   J. Bacteriol. 163:863-869(1985).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-66.
RC   STRAIN=ATCC 33015 / DSM 3931 / JCM 6156 / NCIMB 12182 / mt-2;
RX   PubMed=2430049; DOI=10.1099/00221287-132-5-1347;
RA   Spooner R.A., Lindsay K., Franklin F.C.H.;
RT   "Genetic, functional and sequence analysis of the xylR and xylS regulatory
RT   genes of the TOL plasmid pWW0.";
RL   J. Gen. Microbiol. 132:1347-1358(1986).
CC   -!- FUNCTION: Regulatory protein of the TOL plasmid xyl operons. In the
CC       presence of m-xylene or m-methylbenzyl alcohol XylR activates both the
CC       xylCMABN operon and the regulatory gene xylS; xylS itself activates the
CC       xylXYZLTEGFJQKIH operon. XylR interacts with sigma-54.
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DR   EMBL; M10143; AAB59162.1; -; Genomic_DNA.
DR   EMBL; M15740; AAA26028.2; ALT_SEQ; Genomic_DNA.
DR   PIR; A26084; A26084.
DR   PIR; B24987; B24987.
DR   PIR; JT0327; NIPSRP.
DR   RefSeq; NP_542857.1; NC_003350.1.
DR   RefSeq; WP_011005900.1; NC_003350.1.
DR   AlphaFoldDB; P06519; -.
DR   SMR; P06519; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR024096; NO_sig/Golgi_transp_ligand-bd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR   InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   InterPro; IPR004096; V4R.
DR   InterPro; IPR010523; XylR_N.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   Pfam; PF02830; V4R; 1.
DR   Pfam; PF06505; XylR_N; 1.
DR   PRINTS; PR01590; HTHFIS.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00989; V4R; 1.
DR   SUPFAM; SSF111126; SSF111126; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   4: Predicted;
KW   Activator; Aromatic hydrocarbons catabolism; ATP-binding; DNA-binding;
KW   Nucleotide-binding; Plasmid; Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN           1..566
FT                   /note="Transcriptional regulatory protein XylR"
FT                   /id="PRO_0000081344"
FT   DOMAIN          235..464
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   DNA_BIND        534..553
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   BINDING         263..270
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         326..335
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
SQ   SEQUENCE   566 AA;  63744 MW;  0A4AD82A02C0747D CRC64;
     MSLTYKPKMQ HEDMQDLSSQ IRFVAAEGKI WLGEQRMLVM QLSTLASFRR EIISLIGVER
     AKGFFLRLGY QSGLMDAELA RKLRPAMREE EVFLAGPQLY ALKGMVKVRL LTMDIAIRDG
     RFNVEAEWID SFEVDICRTE LGLMNEPVCW TVLGYASGYG SAFMGRRIIF QETSCRGCGD
     DKCLIVGKTA EEWGDVSSFE AYFKSDPIVD ERYELQTQVA NLRNRLKQYD GQYYGIGHSP
     AYKRICETID KAARGRVSVL LLGETGVGKE VIARSVHLRS ERAEQPFVAV NCAAIPPDLI
     ESELFGVDKG AYTGAVNARA GRFERANGGT IFLDEVIELT PRAQATLLRV LQEGELERVG
     GDRTRKVDVR LITATNENLE EAVKMGRFRA DLFFRLNVFP VHIPPLRERV EDIPLLVEHF
     LRRHHKEYGK KTLGLSDRAM EACLHYQWPG NIRELENALE RGVILTESNE SINVESLFPG
     LATATEGDRL SSEGRLEEES GDSWFRQIID QGVSLEDLEA GLMRTAMDRC GQNISQAARL
     LGLTRPAMAY RLKKLDPSLS VKAMGR