ID   XYLS1_PSEPU             Reviewed;         321 AA.
AC   Q04710;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=XylDLEGF operon transcriptional activator 1;
GN   Name=xylS1;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OG   Plasmid TOL pWW53.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MT53;
RX   PubMed=8473862; DOI=10.1099/00221287-139-3-557;
RA   Assinder S.J., de Marco P., Osborne D.J., Poh C.L., Shaw L.E., Winson M.K.,
RA   Williams P.A.;
RT   "A comparison of the multiple alleles of xylS carried by TOL plasmids pWW53
RT   and pDK1 and its implications for their evolutionary relationship.";
RL   J. Gen. Microbiol. 139:557-568(1993).
CC   -!- FUNCTION: Regulatory protein of the TOL plasmid xyl operons. XylS
CC       activates the xylXYZLTEGFJQKIH operon required for the degradation of
CC       toluene, m-xylene and p-xylene.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
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DR   EMBL; L02356; AAA70302.1; -; Genomic_DNA.
DR   PIR; A47695; A47695.
DR   RefSeq; WP_004575911.1; NZ_MING01000087.1.
DR   RefSeq; YP_709330.1; NC_008275.1.
DR   AlphaFoldDB; Q04710; -.
DR   SMR; Q04710; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0009893; P:positive regulation of metabolic process; IEA:UniProt.
DR   InterPro; IPR035418; AraC-bd_2.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR037923; HTH-like.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR018062; HTH_AraC-typ_CS.
DR   Pfam; PF14525; AraC_binding_2; 1.
DR   Pfam; PF12833; HTH_18; 1.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF51215; SSF51215; 1.
DR   PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   4: Predicted;
KW   Activator; Aromatic hydrocarbons catabolism; Cytoplasm; DNA-binding;
KW   Plasmid; Transcription; Transcription regulation.
FT   CHAIN           1..321
FT                   /note="XylDLEGF operon transcriptional activator 1"
FT                   /id="PRO_0000194602"
FT   DOMAIN          214..315
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        231..252
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        282..305
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
SQ   SEQUENCE   321 AA;  36582 MW;  63B87F1C569E57B7 CRC64;
     MDFRLLNEKS QIFVHAEPYA VSDYVNQYVG THSIRLPKGG RPAGRLHHRI FGGLDLCRIS
     YGGSVRVISP GLETCYHLQI ILKGHCLWRD HGQEHYFAPG ELLLLNPDDQ ADLTYSEDCE
     KFIVKLPSVV LDRACSENNW HKPREGIRFA ARHNLQQLDG FINLLGLVCD EAEHTKSMPR
     VQEHYAGIIA SKLLEMLGSN VSREIFSKGN PSFERVVQFI EENLKRNISL ERLAELAMMS
     PRSLYNLFEK HAGTTPKNYI RNRKLESIRA CLNDPSANVR SITEIALDYG FLHLGRFAEN
     YRSAFGELPS DTLRQCKKEV A