ID   XYLS_PSEPU              Reviewed;         321 AA.
AC   P07859;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=XylDLEGF operon transcriptional activator;
GN   Name=xylS;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OG   Plasmid TOL pWW0.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3023186; DOI=10.1016/0378-1119(86)90187-3;
RA   Inouye S., Nakazawa A., Nakazawa T.;
RT   "Nucleotide sequence of the regulatory gene xylS on the Pseudomonas putida
RT   TOL plasmid and identification of the protein product.";
RL   Gene 44:235-242(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3475526; DOI=10.1007/bf00331600;
RA   Mermod N., Ramos J.L., Bairoch A., Timmis K.N.;
RT   "The xylS gene positive regulator of TOL plasmid pWWO: identification,
RT   sequence analysis and overproduction leading to constitutive expression of
RT   meta cleavage operon.";
RL   Mol. Gen. Genet. 207:349-354(1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33015 / DSM 3931 / JCM 6156 / NCIMB 12182 / mt-2;
RX   PubMed=2430049; DOI=10.1099/00221287-132-5-1347;
RA   Spooner R.A., Lindsay K., Franklin F.C.H.;
RT   "Genetic, functional and sequence analysis of the xylR and xylS regulatory
RT   genes of the TOL plasmid pWW0.";
RL   J. Gen. Microbiol. 132:1347-1358(1986).
CC   -!- FUNCTION: Regulatory protein of the TOL plasmid xyl operons. XylS
CC       activates the xylXYZLTEGFJQKIH operon required for the degradation of
CC       toluene, m-xylene and p-xylene.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
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DR   EMBL; M10143; AAB59163.1; -; Genomic_DNA.
DR   EMBL; M15740; AAA26029.1; -; Genomic_DNA.
DR   PIR; A24987; A24987.
DR   RefSeq; NP_542858.1; NC_003350.1.
DR   RefSeq; WP_011005901.1; NZ_QWEF01000005.1.
DR   AlphaFoldDB; P07859; -.
DR   SMR; P07859; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0009893; P:positive regulation of metabolic process; IEA:UniProt.
DR   InterPro; IPR035418; AraC-bd_2.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR037923; HTH-like.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR018062; HTH_AraC-typ_CS.
DR   Pfam; PF14525; AraC_binding_2; 1.
DR   Pfam; PF12833; HTH_18; 1.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF51215; SSF51215; 1.
DR   PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   4: Predicted;
KW   Activator; Aromatic hydrocarbons catabolism; Cytoplasm; DNA-binding;
KW   Plasmid; Transcription; Transcription regulation.
FT   CHAIN           1..321
FT                   /note="XylDLEGF operon transcriptional activator"
FT                   /id="PRO_0000194601"
FT   DOMAIN          214..315
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        231..252
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        282..305
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
SQ   SEQUENCE   321 AA;  36503 MW;  D015933FB5A070FE CRC64;
     MDFCLLNEKS QIFVHAEPYA VSDYVNQYVG THSIRLPKGG RPAGRLHHRI FGCLDLCRIS
     YGGSVRVISP GLETCYHLQI ILKGHCLWRG HGQEHYFAPG ELLLLNPDDQ ADLTYSEDCE
     KFIVKLPSVV LDRACSDNNW HKPREGIRFA ARHNLQQLDG FINLLGLVCD EAEHTKSMPR
     VQEHYAGIIA SKLLEMLGSN VSREIFSKGN PSFERVVQFI EENLKRNISL ERLAELAMMS
     PRSLYNLFEK HAGTTPKNYI RNRKLESIRA CLNDPSANVR SITEIALDYG FLHLGRFAEN
     YRSAFGELPS DTLRQCKKEV A