ID   CAPD_RICPR              Reviewed;         341 AA.
AC   Q9ZDJ5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=UDP-glucose 4-epimerase;
DE            EC=5.1.3.2;
DE   AltName: Full=Galactowaldenase;
DE   AltName: Full=UDP-galactose 4-epimerase;
GN   Name=capD; OrderedLocusNames=RP333;
OS   Rickettsia prowazekii (strain Madrid E).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX   NCBI_TaxID=272947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Madrid E;
RX   PubMed=9823893; DOI=10.1038/24094;
RA   Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T.,
RA   Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H.,
RA   Kurland C.G.;
RT   "The genome sequence of Rickettsia prowazekii and the origin of
RT   mitochondria.";
RL   Nature 396:133-140(1998).
RN   [2]
RP   FUNCTION AS AN UDP-GLUCOSE EPIMERASE.
RC   STRAIN=ATCC VR-142 / Breinl;
RX   PubMed=16386381; DOI=10.1016/j.gene.2005.10.030;
RA   Santhanagopalan V., Coker C., Radulovic S.;
RT   "Characterization of RP 333, a gene encoding CapD of Rickettsia prowazekii
RT   with UDP-glucose 4-epimerase activity.";
RL   Gene 369:119-125(2006).
CC   -!- FUNCTION: Epimerizes UDP-galactose to UDP-glucose. May contribute to
CC       formation of LPS or the exopolysaccharide slime layer by providing UDP-
CC       galactose as a substrate for either molecule.
CC       {ECO:0000269|PubMed:16386381}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=UDP-alpha-D-glucose = UDP-alpha-D-galactose;
CC         Xref=Rhea:RHEA:22168, ChEBI:CHEBI:58885, ChEBI:CHEBI:66914;
CC         EC=5.1.3.2;
CC   -!- MISCELLANEOUS: Addition of NAD/NADP does not result in differences in
CC       enzymatic activity.
CC   -!- MISCELLANEOUS: Gene transcripts were detected at ten days post-
CC       infection in chicken yolk sacs infected with strain Breinl.
CC   -!- SIMILARITY: Belongs to the polysaccharide synthase family.
CC       {ECO:0000305}.
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DR   EMBL; AJ235271; CAA14793.1; -; Genomic_DNA.
DR   PIR; G71689; G71689.
DR   RefSeq; NP_220716.1; NC_000963.1.
DR   RefSeq; WP_004597459.1; NC_000963.1.
DR   AlphaFoldDB; Q9ZDJ5; -.
DR   SMR; Q9ZDJ5; -.
DR   STRING; 272947.RP333; -.
DR   EnsemblBacteria; CAA14793; CAA14793; CAA14793.
DR   GeneID; 57569459; -.
DR   KEGG; rpr:RP333; -.
DR   PATRIC; fig|272947.5.peg.343; -.
DR   eggNOG; COG1086; Bacteria.
DR   HOGENOM; CLU_013560_4_1_5; -.
DR   OMA; HEVMIPK; -.
DR   Proteomes; UP000002480; Chromosome.
DR   GO; GO:0003978; F:UDP-glucose 4-epimerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd05237; UDP_invert_4-6DH_SDR_e; 1.
DR   InterPro; IPR013692; CapD_C.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR003869; Polysac_CapD-like.
DR   Pfam; PF08485; Polysacc_syn_2C; 1.
DR   Pfam; PF02719; Polysacc_synt_2; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Lipopolysaccharide biosynthesis; Reference proteome.
FT   CHAIN           1..341
FT                   /note="UDP-glucose 4-epimerase"
FT                   /id="PRO_0000314606"
SQ   SEQUENCE   341 AA;  38213 MW;  CA67B07564B9D96B CRC64;
     MFVDKTLMIT GGTGSFGNAV LSRFLKSNII NDIKEIRIFS RDEKKQEDMR IALNNSKLKF
     YIGDVRNYQS IDDAMHGVDY VFHAAALKQV PTCEFYPMEA INTNVLGAEN VLSAAINNKV
     TKVIVLSTDK AVYPINAMGL SKALMEKLAI AKARMRSPGE TILCVTRYGN VMASRGSVIP
     LFIHQIKQGK ELTITEPSMT RFLMSLVDSV DLVLYAFEHG RQGDIFVQKS PASTIEVLAK
     ALQEIFGSKN AIRFIGTRHG EKHYESLVSS EDMAKADDLG GYYRIPMDGR DLNYAKYFVT
     GEKKVALLDD YTSHNTKRLN LKEVKELLLT LDYVQKELKN A