XYNA_ALKHC
ID XYNA_ALKHC Reviewed; 396 AA.
AC P07528; Q9JPV5;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1988, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Endo-1,4-beta-xylanase A;
DE Short=Xylanase A;
DE EC=3.2.1.8;
DE AltName: Full=1,4-beta-D-xylan xylanohydrolase A;
DE Flags: Precursor;
GN Name=xynA; OrderedLocusNames=BH2120;
OS Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344
OS / JCM 9153 / C-125) (Bacillus halodurans).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus.
OX NCBI_TaxID=272558;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF N-TERMINUS.
RC STRAIN=ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125;
RA Hamamoto T., Honda H., Kudo T., Horikoshi K.;
RT "Nucleotide sequence of the xylanase A gene of alkalophilic Bacillus sp.
RT strain C-125.";
RL Agric. Biol. Chem. 51:953-955(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125;
RX PubMed=11058132; DOI=10.1093/nar/28.21.4317;
RA Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F.,
RA Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.;
RT "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans
RT and genomic sequence comparison with Bacillus subtilis.";
RL Nucleic Acids Res. 28:4317-4331(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.;
CC EC=3.2.1.8;
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Active over a very broad pH range.;
CC -!- PATHWAY: Glycan degradation; xylan degradation.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 10 (cellulase F) family.
CC {ECO:0000305}.
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DR EMBL; D00087; BAA00055.1; -; Genomic_DNA.
DR EMBL; BA000004; BAB05839.1; -; Genomic_DNA.
DR PIR; H83914; H83914.
DR PIR; JD0003; JD0003.
DR RefSeq; WP_010898277.1; NC_002570.2.
DR PDB; 2UWF; X-ray; 2.10 A; A=48-396.
DR PDB; 7CPK; X-ray; 1.60 A; A=46-396.
DR PDB; 7CPL; X-ray; 1.52 A; A=46-396.
DR PDBsum; 2UWF; -.
DR PDBsum; 7CPK; -.
DR PDBsum; 7CPL; -.
DR AlphaFoldDB; P07528; -.
DR SMR; P07528; -.
DR STRING; 272558.10174739; -.
DR CAZy; GH10; Glycoside Hydrolase Family 10.
DR EnsemblBacteria; BAB05839; BAB05839; BAB05839.
DR KEGG; bha:BH2120; -.
DR eggNOG; COG3693; Bacteria.
DR HOGENOM; CLU_020161_6_1_9; -.
DR OMA; GIADNHT; -.
DR OrthoDB; 654705at2; -.
DR UniPathway; UPA00114; -.
DR EvolutionaryTrace; P07528; -.
DR Proteomes; UP000001258; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0031176; F:endo-1,4-beta-xylanase activity; IEA:UniProtKB-EC.
DR GO; GO:0045493; P:xylan catabolic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR044846; GH10.
DR InterPro; IPR031158; GH10_AS.
DR InterPro; IPR001000; GH10_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR31490; PTHR31490; 1.
DR Pfam; PF00331; Glyco_hydro_10; 1.
DR PRINTS; PR00134; GLHYDRLASE10.
DR SMART; SM00633; Glyco_10; 1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS00591; GH10_1; 1.
DR PROSITE; PS51760; GH10_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Carbohydrate metabolism; Direct protein sequencing;
KW Glycosidase; Hydrolase; Polysaccharide degradation; Reference proteome;
KW Secreted; Signal; Xylan degradation.
FT SIGNAL 1..28
FT /evidence="ECO:0000269|Ref.1"
FT CHAIN 29..396
FT /note="Endo-1,4-beta-xylanase A"
FT /id="PRO_0000007967"
FT DOMAIN 51..396
FT /note="GH10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01096"
FT ACT_SITE 195
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 301
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10061"
FT HELIX 51..53
FT /evidence="ECO:0007829|PDB:7CPL"
FT HELIX 57..60
FT /evidence="ECO:0007829|PDB:7CPL"
FT TURN 61..64
FT /evidence="ECO:0007829|PDB:7CPL"
FT STRAND 66..71
FT /evidence="ECO:0007829|PDB:7CPL"
FT HELIX 73..75
FT /evidence="ECO:0007829|PDB:7CPL"
FT HELIX 78..87
FT /evidence="ECO:0007829|PDB:7CPL"
FT STRAND 89..95
FT /evidence="ECO:0007829|PDB:7CPL"
FT HELIX 99..102
FT /evidence="ECO:0007829|PDB:7CPL"
FT HELIX 112..123
FT /evidence="ECO:0007829|PDB:7CPL"
FT STRAND 127..130
FT /evidence="ECO:0007829|PDB:7CPL"
FT STRAND 133..138
FT /evidence="ECO:0007829|PDB:7CPL"
FT HELIX 141..144
FT /evidence="ECO:0007829|PDB:7CPL"
FT HELIX 152..154
FT /evidence="ECO:0007829|PDB:7CPL"
FT HELIX 158..183
FT /evidence="ECO:0007829|PDB:7CPL"
FT TURN 184..186
FT /evidence="ECO:0007829|PDB:7CPL"
FT STRAND 188..195
FT /evidence="ECO:0007829|PDB:7CPL"
FT STRAND 201..203
FT /evidence="ECO:0007829|PDB:7CPL"
FT HELIX 207..212
FT /evidence="ECO:0007829|PDB:7CPL"
FT HELIX 215..228
FT /evidence="ECO:0007829|PDB:7CPL"
FT STRAND 234..239
FT /evidence="ECO:0007829|PDB:7CPL"
FT HELIX 244..259
FT /evidence="ECO:0007829|PDB:7CPL"
FT STRAND 266..269
FT /evidence="ECO:0007829|PDB:7CPL"
FT STRAND 272..276
FT /evidence="ECO:0007829|PDB:7CPL"
FT HELIX 280..291
FT /evidence="ECO:0007829|PDB:7CPL"
FT TURN 292..294
FT /evidence="ECO:0007829|PDB:7CPL"
FT STRAND 296..307
FT /evidence="ECO:0007829|PDB:7CPL"
FT HELIX 318..320
FT /evidence="ECO:0007829|PDB:7CPL"
FT HELIX 323..342
FT /evidence="ECO:0007829|PDB:7CPL"
FT HELIX 344..346
FT /evidence="ECO:0007829|PDB:7CPL"
FT STRAND 347..353
FT /evidence="ECO:0007829|PDB:7CPL"
FT HELIX 361..368
FT /evidence="ECO:0007829|PDB:7CPL"
FT TURN 369..372
FT /evidence="ECO:0007829|PDB:7CPL"
FT STRAND 379..381
FT /evidence="ECO:0007829|PDB:7CPL"
FT STRAND 385..387
FT /evidence="ECO:0007829|PDB:7CPL"
FT HELIX 389..395
FT /evidence="ECO:0007829|PDB:7CPL"
SQ SEQUENCE 396 AA; 45294 MW; 29C1F46BE00E180C CRC64;
MITLFRKPFV AGLAISLLVG GGIGNVAAAQ GGPPKSGVFG ENEKRNDQPF AWQVASLSER
YQEQFDIGAA VEPYQLEGRQ AQILKHHYNS LVAENAMKPE SLQPREGEWN WEGADKIVEF
ARKHNMELRF HTLVWHSQVP EWFFIDEDGN RMVDETDPDK REANKQLLLE RMENHIKTVV
ERYKDDVTSW DVVNEVIDDG GGLRESEWYQ ITGTDYIKVA FETARKYGGE EAKLYINDYN
TEVPSKRDDL YNLVKDLLEQ GVPIDGVGHQ SHIQIGWPSI EDTRASFEKF TSLGLDNQVT
ELDMSLYGWP PTGAYTSYDD IPAELLQAQA DRYDQLFELY EELAADISSV TFWGIADNHT
WLDGRAREYN NGVGIDAPFV FDHNYRVKPA YWRIID
