XYNB_GEOSE
ID XYNB_GEOSE Reviewed; 504 AA.
AC Q9ZFM2; Q09LY4;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Beta-xylosidase;
DE EC=3.2.1.37;
DE AltName: Full=1,4-beta-D-xylan xylohydrolase;
DE AltName: Full=Xylan 1,4-beta-xylosidase;
GN Name=xynB;
OS Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX NCBI_TaxID=1422;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=T-6;
RX PubMed=10368143; DOI=10.1128/jb.181.12.3695-3704.1999;
RA Shulami S., Gat O., Sonenshein A.L., Shoham Y.;
RT "The glucuronic acid utilization gene cluster from Bacillus
RT stearothermophilus T-6.";
RL J. Bacteriol. 181:3695-3704(1999).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-
CC xylose residues from the non-reducing termini.; EC=3.2.1.37;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 39 family. {ECO:0000305}.
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DR EMBL; DQ868502; ABI49941.1; -; Genomic_DNA.
DR PDB; 1W91; X-ray; 2.20 A; A/B/C/D/E/F/G/H=1-504.
DR PDB; 2BFG; X-ray; 2.40 A; A/B/C/D/E/F/G/H=1-504.
DR PDB; 2BS9; X-ray; 2.20 A; A/B/C/D/E/F/G/H=1-504.
DR PDBsum; 1W91; -.
DR PDBsum; 2BFG; -.
DR PDBsum; 2BS9; -.
DR AlphaFoldDB; Q9ZFM2; -.
DR SMR; Q9ZFM2; -.
DR CAZy; GH39; Glycoside Hydrolase Family 39.
DR BRENDA; 3.2.1.37; 623.
DR SABIO-RK; Q9ZFM2; -.
DR EvolutionaryTrace; Q9ZFM2; -.
DR GO; GO:0009044; F:xylan 1,4-beta-xylosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0045493; P:xylan catabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR000514; Glyco_hydro_39.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR Pfam; PF01229; Glyco_hydro_39; 1.
DR PRINTS; PR00745; GLHYDRLASE39.
DR SUPFAM; SSF51445; SSF51445; 1.
DR PROSITE; PS01027; GLYCOSYL_HYDROL_F39; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Carbohydrate metabolism; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Xylan degradation.
FT CHAIN 1..504
FT /note="Beta-xylosidase"
FT /id="PRO_0000057687"
FT ACT_SITE 160
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10068"
FT ACT_SITE 280
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT STRAND 3..5
FT /evidence="ECO:0007829|PDB:2BS9"
FT STRAND 11..13
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 17..19
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 20..23
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 27..31
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 33..45
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 49..52
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 56..58
FT /evidence="ECO:0007829|PDB:2BS9"
FT TURN 59..61
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 64..77
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 80..91
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 95..100
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 105..107
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 108..110
FT /evidence="ECO:0007829|PDB:1W91"
FT TURN 115..118
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 127..145
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 147..151
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 153..157
FT /evidence="ECO:0007829|PDB:1W91"
FT TURN 164..166
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 168..170
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 172..189
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 194..200
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 206..217
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 224..230
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 236..238
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 252..267
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 269..271
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 276..283
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 290..293
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 295..307
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 308..310
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 313..318
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 320..322
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 331..336
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 340..342
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 343..345
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 349..358
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 362..368
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 371..375
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 381..386
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 398..405
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 407..423
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 425..431
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 440..448
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 452..459
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 461..463
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 464..471
FT /evidence="ECO:0007829|PDB:1W91"
FT STRAND 476..483
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 488..490
FT /evidence="ECO:0007829|PDB:1W91"
FT HELIX 496..498
FT /evidence="ECO:0007829|PDB:1W91"
SQ SEQUENCE 504 AA; 58119 MW; 59518E75200A18B1 CRC64;
MKVVNVPSNG REKFKKNWKF CVGTGRLGLA LQKEYLDHLK LVQEKIGFRY IRGHGLLSDD
VGIYREVEID GEMKPFYNFT YIDRIVDSYL ALNIRPFIEF GFMPKALASG DQTVFYWKGN
VTPPKDYNKW RDLIVAVVSH FIERYGIEEV RTWLFEVWNE PNLVNFWKDA NKQEYFKLYE
VTARAVKSVD PHLQVGGPAI CGGSDEWITD FLHFCAERRV PVDFVSRHAY TSKAPHKKTF
EYYYQELELE PPEDMLEQFK TVRALIRQSP FPHLPLHITE YNTSYSPINP VHDTALNAAY
IARILSEGGD YVDSFSYWTF SDVFEEMDVP KALFHGGFGL VALHSIPKPT FHAFTFFNAL
GDELLYRDGE MIVTRRKDGS IAAVLWNLVM EKGEGLTKEV QLVIPVSFSA VFIKRQIVNE
QYGNAWRVWK QMGRPRFPSR QAVETLPSAQ PHVMTEQRRA TDGVIHLSIV LSKNEVTLIE
IEQVRDETST YVGLDDGEIT SYSS
