XYNY_ACETH
ID XYNY_ACETH Reviewed; 1077 AA.
AC P51584;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 25-MAY-2022, entry version 138.
DE RecName: Full=Endo-1,4-beta-xylanase Y;
DE Short=Xylanase Y;
DE EC=3.2.1.8;
DE AltName: Full=1,4-beta-D-xylan xylanohydrolase Y;
DE Short=XylY;
DE Flags: Precursor;
GN Name=xynY;
OS Acetivibrio thermocellus (Hungateiclostridium thermocellum) (Clostridium
OS thermocellum).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC Acetivibrio.
OX NCBI_TaxID=1515;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YS;
RX PubMed=7717969; DOI=10.1042/bj3070151;
RA Fontes C.M.G.A., Hazelwood G.P., Morag E., Hall J., Hirst B.H.,
RA Gilbert H.J.;
RT "Evidence for a general role for non-catalytic thermostabilizing domains in
RT xylanases from thermophilic bacteria.";
RL Biochem. J. 307:151-158(1995).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.;
CC EC=3.2.1.8;
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 6.8.;
CC Temperature dependence:
CC Optimum temperature is 75 degrees Celsius.;
CC -!- INTERACTION:
CC P51584; Q06851: cipA; Xeno; NbExp=5; IntAct=EBI-1037473, EBI-687595;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 10 (cellulase F) family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X83269; CAA58242.1; -; Genomic_DNA.
DR PIR; S54975; S54975.
DR PDB; 1DYO; X-ray; 2.10 A; A/B=558-718.
DR PDB; 1GKK; X-ray; 1.60 A; A/B=792-1077.
DR PDB; 1GKL; X-ray; 1.40 A; A/B=792-1077.
DR PDB; 1H6X; X-ray; 2.23 A; A=560-720.
DR PDB; 1H6Y; X-ray; 2.12 A; A/B=560-720.
DR PDB; 1OHZ; X-ray; 2.20 A; B=733-791.
DR PDB; 1WB4; X-ray; 1.40 A; A/B=792-1077.
DR PDB; 1WB5; X-ray; 1.40 A; A/B=792-1077.
DR PDB; 1WB6; X-ray; 1.40 A; A/B=792-1077.
DR PDB; 2CCL; X-ray; 2.03 A; B/D=730-791.
DR PDB; 2W5F; X-ray; 1.90 A; A/B=32-551.
DR PDB; 2WYS; X-ray; 2.75 A; A/B=33-551.
DR PDB; 2WZE; X-ray; 2.50 A; A/B=33-551.
DR PDB; 3ZI7; X-ray; 2.30 A; A/B=792-1077.
DR PDB; 4BAG; X-ray; 1.90 A; A/B=792-1077.
DR PDB; 4H35; X-ray; 1.90 A; A/B=792-1077.
DR PDB; 5FXM; X-ray; 1.99 A; A=792-1077.
DR PDB; 6FJ4; X-ray; 1.70 A; A=803-1077.
DR PDB; 6Y8G; X-ray; 1.80 A; AAA/BBB=792-1077.
DR PDBsum; 1DYO; -.
DR PDBsum; 1GKK; -.
DR PDBsum; 1GKL; -.
DR PDBsum; 1H6X; -.
DR PDBsum; 1H6Y; -.
DR PDBsum; 1OHZ; -.
DR PDBsum; 1WB4; -.
DR PDBsum; 1WB5; -.
DR PDBsum; 1WB6; -.
DR PDBsum; 2CCL; -.
DR PDBsum; 2W5F; -.
DR PDBsum; 2WYS; -.
DR PDBsum; 2WZE; -.
DR PDBsum; 3ZI7; -.
DR PDBsum; 4BAG; -.
DR PDBsum; 4H35; -.
DR PDBsum; 5FXM; -.
DR PDBsum; 6FJ4; -.
DR PDBsum; 6Y8G; -.
DR AlphaFoldDB; P51584; -.
DR SMR; P51584; -.
DR DIP; DIP-35413N; -.
DR IntAct; P51584; 1.
DR DrugBank; DB04522; Dexfosfoserine.
DR DrugBank; DB07767; Ferulic acid.
DR DrugBank; DB08711; Methyl vanillate.
DR DrugBank; DB08587; Sinapic acid.
DR DrugBank; DB08589; SYRINGATE.
DR CAZy; CBM22; Carbohydrate-Binding Module Family 22.
DR CAZy; GH10; Glycoside Hydrolase Family 10.
DR ESTHER; clotm-xyny; A85-Feruloyl-Esterase.
DR BRENDA; 3.2.1.8; 1530.
DR EvolutionaryTrace; P51584; -.
DR GO; GO:0043263; C:cellulosome; IDA:MENGO.
DR GO; GO:0031176; F:endo-1,4-beta-xylanase activity; IEA:UniProtKB-EC.
DR GO; GO:0045493; P:xylan catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1330.10; -; 1.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR003305; CenC_carb-bd.
DR InterPro; IPR002105; Dockerin_1_rpt.
DR InterPro; IPR016134; Dockerin_dom.
DR InterPro; IPR036439; Dockerin_dom_sf.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR000801; Esterase-like.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR044846; GH10.
DR InterPro; IPR031158; GH10_AS.
DR InterPro; IPR001000; GH10_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR31490; PTHR31490; 1.
DR Pfam; PF02018; CBM_4_9; 2.
DR Pfam; PF00404; Dockerin_1; 1.
DR Pfam; PF00756; Esterase; 1.
DR Pfam; PF00331; Glyco_hydro_10; 1.
DR PRINTS; PR00134; GLHYDRLASE10.
DR SMART; SM00633; Glyco_10; 1.
DR SUPFAM; SSF49785; SSF49785; 2.
DR SUPFAM; SSF51445; SSF51445; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR SUPFAM; SSF63446; SSF63446; 1.
DR PROSITE; PS00448; CLOS_CELLULOSOME_RPT; 2.
DR PROSITE; PS51766; DOCKERIN; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS00591; GH10_1; 1.
DR PROSITE; PS51760; GH10_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Carbohydrate metabolism; Glycosidase; Hydrolase;
KW Polysaccharide degradation; Repeat; Signal; Xylan degradation.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..1077
FT /note="Endo-1,4-beta-xylanase Y"
FT /id="PRO_0000007972"
FT DOMAIN 33..180
FT /note="CBM-cenC 1"
FT DOMAIN 189..538
FT /note="GH10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01096"
FT DOMAIN 565..714
FT /note="CBM-cenC 2"
FT DOMAIN 728..796
FT /note="Dockerin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01102"
FT REGION 543..563
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 337
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 460
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10061"
FT STRAND 34..39
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 42..44
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 48..52
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 56..60
FT /evidence="ECO:0007829|PDB:2W5F"
FT HELIX 65..67
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 70..74
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 78..80
FT /evidence="ECO:0007829|PDB:2WYS"
FT STRAND 82..86
FT /evidence="ECO:0007829|PDB:2W5F"
FT TURN 88..90
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 96..104
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 107..121
FT /evidence="ECO:0007829|PDB:2W5F"
FT TURN 122..124
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 127..138
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 143..150
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 155..166
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 170..179
FT /evidence="ECO:0007829|PDB:2W5F"
FT HELIX 195..198
FT /evidence="ECO:0007829|PDB:2W5F"
FT TURN 199..202
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 204..209
FT /evidence="ECO:0007829|PDB:2W5F"
FT HELIX 213..215
FT /evidence="ECO:0007829|PDB:2W5F"
FT HELIX 217..226
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 228..234
FT /evidence="ECO:0007829|PDB:2W5F"
FT HELIX 238..241
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 242..248
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 251..254
FT /evidence="ECO:0007829|PDB:2W5F"
FT TURN 257..259
FT /evidence="ECO:0007829|PDB:2W5F"
FT HELIX 260..268
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 272..279
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 281..283
FT /evidence="ECO:0007829|PDB:2W5F"
FT HELIX 286..289
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 293..297
FT /evidence="ECO:0007829|PDB:2WYS"
FT HELIX 302..323
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 329..337
FT /evidence="ECO:0007829|PDB:2W5F"
FT HELIX 343..348
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 355..358
FT /evidence="ECO:0007829|PDB:2W5F"
FT HELIX 363..368
FT /evidence="ECO:0007829|PDB:2W5F"
FT HELIX 373..384
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 390..397
FT /evidence="ECO:0007829|PDB:2W5F"
FT HELIX 401..416
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 422..425
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 428..431
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 433..435
FT /evidence="ECO:0007829|PDB:2W5F"
FT HELIX 439..450
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 453..464
FT /evidence="ECO:0007829|PDB:2W5F"
FT TURN 466..469
FT /evidence="ECO:0007829|PDB:2W5F"
FT HELIX 472..492
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 498..507
FT /evidence="ECO:0007829|PDB:2W5F"
FT HELIX 515..517
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 520..522
FT /evidence="ECO:0007829|PDB:2W5F"
FT HELIX 530..535
FT /evidence="ECO:0007829|PDB:2W5F"
FT HELIX 541..543
FT /evidence="ECO:0007829|PDB:2W5F"
FT STRAND 566..571
FT /evidence="ECO:0007829|PDB:1DYO"
FT STRAND 581..583
FT /evidence="ECO:0007829|PDB:1DYO"
FT STRAND 587..593
FT /evidence="ECO:0007829|PDB:1DYO"
FT STRAND 596..599
FT /evidence="ECO:0007829|PDB:1DYO"
FT STRAND 601..605
FT /evidence="ECO:0007829|PDB:1DYO"
FT STRAND 613..618
FT /evidence="ECO:0007829|PDB:1DYO"
FT TURN 620..622
FT /evidence="ECO:0007829|PDB:1DYO"
FT STRAND 628..636
FT /evidence="ECO:0007829|PDB:1DYO"
FT STRAND 639..642
FT /evidence="ECO:0007829|PDB:1DYO"
FT STRAND 644..653
FT /evidence="ECO:0007829|PDB:1DYO"
FT STRAND 659..669
FT /evidence="ECO:0007829|PDB:1DYO"
FT STRAND 675..683
FT /evidence="ECO:0007829|PDB:1DYO"
FT STRAND 689..699
FT /evidence="ECO:0007829|PDB:1DYO"
FT STRAND 704..713
FT /evidence="ECO:0007829|PDB:1DYO"
FT STRAND 738..740
FT /evidence="ECO:0007829|PDB:2CCL"
FT HELIX 743..753
FT /evidence="ECO:0007829|PDB:2CCL"
FT HELIX 761..767
FT /evidence="ECO:0007829|PDB:2CCL"
FT HELIX 777..787
FT /evidence="ECO:0007829|PDB:2CCL"
FT HELIX 817..820
FT /evidence="ECO:0007829|PDB:1GKL"
FT STRAND 828..836
FT /evidence="ECO:0007829|PDB:1GKL"
FT STRAND 839..847
FT /evidence="ECO:0007829|PDB:1GKL"
FT STRAND 858..863
FT /evidence="ECO:0007829|PDB:1GKL"
FT TURN 876..878
FT /evidence="ECO:0007829|PDB:1GKL"
FT HELIX 880..889
FT /evidence="ECO:0007829|PDB:1GKL"
FT STRAND 896..900
FT /evidence="ECO:0007829|PDB:1GKL"
FT TURN 910..912
FT /evidence="ECO:0007829|PDB:1GKL"
FT HELIX 913..919
FT /evidence="ECO:0007829|PDB:1GKL"
FT HELIX 921..928
FT /evidence="ECO:0007829|PDB:1GKL"
FT STRAND 934..937
FT /evidence="ECO:0007829|PDB:3ZI7"
FT HELIX 938..942
FT /evidence="ECO:0007829|PDB:1GKL"
FT HELIX 943..947
FT /evidence="ECO:0007829|PDB:1GKL"
FT STRAND 948..953
FT /evidence="ECO:0007829|PDB:1GKL"
FT HELIX 955..967
FT /evidence="ECO:0007829|PDB:1GKL"
FT TURN 968..970
FT /evidence="ECO:0007829|PDB:1GKL"
FT STRAND 973..978
FT /evidence="ECO:0007829|PDB:1GKL"
FT STRAND 983..986
FT /evidence="ECO:0007829|PDB:1GKL"
FT HELIX 987..1001
FT /evidence="ECO:0007829|PDB:1GKL"
FT STRAND 1009..1015
FT /evidence="ECO:0007829|PDB:1GKL"
FT HELIX 1021..1032
FT /evidence="ECO:0007829|PDB:1GKL"
FT STRAND 1039..1041
FT /evidence="ECO:0007829|PDB:1GKL"
FT TURN 1043..1045
FT /evidence="ECO:0007829|PDB:1GKL"
FT STRAND 1048..1053
FT /evidence="ECO:0007829|PDB:1GKL"
FT HELIX 1060..1070
FT /evidence="ECO:0007829|PDB:1GKL"
FT HELIX 1071..1073
FT /evidence="ECO:0007829|PDB:1GKL"
SQ SEQUENCE 1077 AA; 119673 MW; BFC8D2D22C5726A0 CRC64;
MKNKRVLAKI TALVVLLGVF FVLPSNISQL YADYEVVHDT FEVNFDGWCN LGVDTYLTAV
ENEGNNGTRG MMVINRSSAS DGAYSEKGFY LDGGVEYKYS VFVKHNGTGT ETFKLSVSYL
DSETEEENKE VIATKDVVAG EWTEISAKYK APKTAVNITL SITTDSTVDF IFDDVTITRK
GMAEANTVYA ANAVLKDMYA NYFRVGSVLN SGTVNNSSIK ALILREFNSI TCENEMKPDA
TLVQSGSTNT NIRVSLNRAA SILNFCAQNN IAVRGHTLVW HSQTPQWFFK DNFQDNGNWV
SQSVMDQRLE SYIKNMFAEI QRQYPSLNLY AYDVVNEAVS DDANRTRYYG GAREPGYGNG
RSPWVQIYGD NKFIEKAFTY ARKYAPANCK LYYNDYNEYW DHKRDCIASI CANLYNKGLL
DGVGMQSHIN ADMNGFSGIQ NYKAALQKYI NIGCDVQITE LDISTENGKF SLQQQADKYK
AVFQAAVDIN RTSSKGKVTA VCVWGPNDAN TWLGSQNAPL LFNANNQPKP AYNAVASIIP
QSEWGDGNNP AGGGGGGKPE EPDANGYYYH DTFEGSVGQW TARGPAEVLL SGRTAYKGSE
SLLVRNRTAA WNGAQRALNP RTFVPGNTYC FSVVASFIEG ASSTTFCMKL QYVDGSGTQR
YDTIDMKTVG PNQWVHLYNP QYRIPSDATD MYVYVETADD TINFYIDEAI GAVAGTVIEG
PAPQPTQPPV LLGDVNGDGT INSTDLTMLK RSVLRAITLT DDAKARADVD KNGSINSTDV
LLLSRYLLRV IDKFPVAENP SSSFKYESAV QYRPAPDSYL NPCPQAGRIV KETYTGINGT
KSLNVYLPYG YDPNKKYNIF YLMHGGGENE NTIFSNDVKL QNILDHAIMN GELEPLIVVT
PTFNGGNCTA QNFYQEFRQN VIPFVESKYS TYAESTTPQG IAASRMHRGF GGFSMGGLTT
WYVMVNCLDY VAYFMPLSGD YWYGNSPQDK ANSIAEAINR SGLSKREYFV FAATGSDHIA
YANMNPQIEA MKALPHFDYT SDFSKGNFYF LVAPGATHWW GYVRHYIYDA LPYFFHE
