ID   XYN_TRIHA               Reviewed;         190 AA.
AC   P48793;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Endo-1,4-beta-xylanase;
DE            Short=Xylanase;
DE            EC=3.2.1.8;
DE   AltName: Full=1,4-beta-D-xylan xylanohydrolase;
OS   Trichoderma harzianum (Hypocrea lixii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma.
OX   NCBI_TaxID=5544;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=E58;
RA   Yaguchi M., Roy C., Watson D.C., Rollin F., Tan L.U.L., Senior D.J.,
RA   Saddler J.N.;
RT   "The amino acid sequence of the 20 Kd xylanase from Trichoderma harzianum
RT   E58.";
RL   (In) Visser J., Beldman G., Kusters-van Someren M.A., Voragen A.G.J.
RL   (eds.);
RL   Xylans and xylanases, pp.435-438, Elsevier, Amsterdam (1992).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
RA   Campbell R.L., Rose D.R., Wakarchuk W.W., To R.J., Sung W., Yaguchi M.;
RT   "High-resolution structures of xylanases from B.circulans and T.harzianum
RT   identify a new folding pattern and implications for the atomic basis of the
RT   catalysis.";
RL   (In) Suominen P., Reinikainen T. (eds.);
RL   Trichoderma reesei cellulases and other hydrolases, pp.63-72, Foundation
RL   for Biotechnical and Industrial Fermentation Research, Helsinki (1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.;
CC         EC=3.2.1.8;
CC   -!- PATHWAY: Glycan degradation; xylan degradation.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 11 (cellulase G) family.
CC       {ECO:0000305}.
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DR   PIR; A44593; A44593.
DR   PDB; 1XND; X-ray; 2.00 A; A=1-190.
DR   PDBsum; 1XND; -.
DR   AlphaFoldDB; P48793; -.
DR   SMR; P48793; -.
DR   CAZy; GH11; Glycoside Hydrolase Family 11.
DR   CLAE; XYN11A_TRIHA; -.
DR   UniPathway; UPA00114; -.
DR   EvolutionaryTrace; P48793; -.
DR   GO; GO:0031176; F:endo-1,4-beta-xylanase activity; IEA:UniProtKB-EC.
DR   GO; GO:0045493; P:xylan catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.60.120.180; -; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR013319; GH11/12.
DR   InterPro; IPR018208; GH11_AS_1.
DR   InterPro; IPR033119; GH11_AS_2.
DR   InterPro; IPR033123; GH11_dom.
DR   InterPro; IPR001137; Glyco_hydro_11.
DR   Pfam; PF00457; Glyco_hydro_11; 1.
DR   PRINTS; PR00911; GLHYDRLASE11.
DR   SUPFAM; SSF49899; SSF49899; 1.
DR   PROSITE; PS00776; GH11_1; 1.
DR   PROSITE; PS00777; GH11_2; 1.
DR   PROSITE; PS51761; GH11_3; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Carbohydrate metabolism; Direct protein sequencing;
KW   Glycosidase; Hydrolase; Polysaccharide degradation; Xylan degradation.
FT   CHAIN           1..190
FT                   /note="Endo-1,4-beta-xylanase"
FT                   /id="PRO_0000184072"
FT   DOMAIN          1..190
FT                   /note="GH11"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01097"
FT   ACT_SITE        86
FT                   /note="Nucleophile"
FT   ACT_SITE        177
FT                   /note="Proton donor"
FT   STRAND          5..12
FT                   /evidence="ECO:0007829|PDB:1XND"
FT   STRAND          14..19
FT                   /evidence="ECO:0007829|PDB:1XND"
FT   STRAND          25..29
FT                   /evidence="ECO:0007829|PDB:1XND"
FT   STRAND          34..41
FT                   /evidence="ECO:0007829|PDB:1XND"
FT   STRAND          44..53
FT                   /evidence="ECO:0007829|PDB:1XND"
FT   STRAND          59..81
FT                   /evidence="ECO:0007829|PDB:1XND"
FT   TURN            82..84
FT                   /evidence="ECO:0007829|PDB:1XND"
FT   STRAND          85..93
FT                   /evidence="ECO:0007829|PDB:1XND"
FT   TURN            98..101
FT                   /evidence="ECO:0007829|PDB:1XND"
FT   STRAND          103..110
FT                   /evidence="ECO:0007829|PDB:1XND"
FT   STRAND          113..125
FT                   /evidence="ECO:0007829|PDB:1XND"
FT   STRAND          130..143
FT                   /evidence="ECO:0007829|PDB:1XND"
FT   STRAND          146..151
FT                   /evidence="ECO:0007829|PDB:1XND"
FT   HELIX           152..161
FT                   /evidence="ECO:0007829|PDB:1XND"
FT   STRAND          168..180
FT                   /evidence="ECO:0007829|PDB:1XND"
FT   STRAND          182..189
FT                   /evidence="ECO:0007829|PDB:1XND"
SQ   SEQUENCE   190 AA;  20703 MW;  6A0F4D1C3599C698 CRC64;
     QTIGPGTGYS NGYYYSYWND GHAGVTYTNG GGGSFTVNWS NSGNFVAGKG WQPGTKNKVI
     NFSGSYNPNG NSYLSIYGWS RNPLIEYYIV ENFGTYNPST GATKLGEVTS DGSVYDIYRT
     QRVNQPSIIG TATFYQYWSV RRNHRSSGSV NTANHFNAWA SHGLTLGTMD YQIVAVEGYF
     SSGSASITVS