XYXT1_ORYSJ
ID XYXT1_ORYSJ Reviewed; 465 AA.
AC Q5Z8T8; Q0D9N6;
DT 05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Beta-1,2-xylosyltransferase XYXT1 {ECO:0000305};
DE EC=2.4.2.- {ECO:0000269|PubMed:29325159};
DE AltName: Full=Xylan xylosyltransferase 1 {ECO:0000303|PubMed:29325159};
GN Name=XYXT1 {ECO:0000303|PubMed:29325159};
GN OrderedLocusNames=Os06g0707000 {ECO:0000312|EMBL:BAS99411.1},
GN LOC_Os06g49300 {ECO:0000305};
GN ORFNames=OsJ_22601 {ECO:0000312|EMBL:EAZ38226.1},
GN P0018H04.33 {ECO:0000312|EMBL:BAD53816.1},
GN P0621D05.21 {ECO:0000312|EMBL:BAD53603.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR
RP LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=29325159; DOI=10.1093/pcp/pcy003;
RA Zhong R., Cui D., Phillips D.R., Ye Z.H.;
RT "A novel rice xylosyltransferase catalyzes the addition of 2-O-xylosyl side
RT chains onto the xylan backbone.";
RL Plant Cell Physiol. 59:554-565(2018).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Glycosyltransferase involved in the xylosylation of xylan,
CC the major hemicellulose (non-cellulosic component) of primary and
CC secondary walls of angiosperms (PubMed:29325159). Possesses beta-1,2-
CC xylosyltransferase activity, transferring xylose from UDP-xylose to the
CC xylan backbone (PubMed:29325159). Catalyzes the addition of 2-O-xylosyl
CC side chains to the xylan backbone (PubMed:29325159).
CC {ECO:0000269|PubMed:29325159}.
CC -!- PATHWAY: Glycan metabolism. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:29325159}; Single-pass type II membrane protein
CC {ECO:0000305|PubMed:29325159}.
CC -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:29325159}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 61 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF20437.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; MG763173; AUR26647.1; -; mRNA.
DR EMBL; AP003621; BAD53603.1; -; Genomic_DNA.
DR EMBL; AP003761; BAD53816.1; -; Genomic_DNA.
DR EMBL; AP008212; BAF20437.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014962; BAS99411.1; -; Genomic_DNA.
DR EMBL; CM000143; EAZ38226.1; -; Genomic_DNA.
DR RefSeq; XP_015642125.1; XM_015786639.1.
DR RefSeq; XP_015642126.1; XM_015786640.1.
DR AlphaFoldDB; Q5Z8T8; -.
DR SMR; Q5Z8T8; -.
DR CAZy; GT61; Glycosyltransferase Family 61.
DR PaxDb; Q5Z8T8; -.
DR PRIDE; Q5Z8T8; -.
DR EnsemblPlants; Os06t0707000-00; Os06t0707000-00; Os06g0707000.
DR GeneID; 4342010; -.
DR Gramene; Os06t0707000-00; Os06t0707000-00; Os06g0707000.
DR KEGG; osa:4342010; -.
DR eggNOG; KOG4698; Eukaryota.
DR HOGENOM; CLU_016869_3_2_1; -.
DR InParanoid; Q5Z8T8; -.
DR OMA; EQRVHCY; -.
DR OrthoDB; 567582at2759; -.
DR BRENDA; 2.4.2.38; 8948.
DR Proteomes; UP000000763; Chromosome 6.
DR Proteomes; UP000007752; Chromosome 6.
DR Proteomes; UP000059680; Chromosome 6.
DR GO; GO:0000139; C:Golgi membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR GO; GO:0035252; F:UDP-xylosyltransferase activity; IDA:UniProtKB.
DR GO; GO:0009664; P:plant-type cell wall organization; IMP:UniProtKB.
DR InterPro; IPR007657; Glycosyltransferase_61.
DR PANTHER; PTHR20961; PTHR20961; 1.
DR Pfam; PF04577; Glyco_transf_61; 1.
PE 1: Evidence at protein level;
KW Cell wall biogenesis/degradation; Glycoprotein; Glycosyltransferase;
KW Golgi apparatus; Membrane; Reference proteome; Signal-anchor; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..465
FT /note="Beta-1,2-xylosyltransferase XYXT1"
FT /id="PRO_0000445785"
FT TOPO_DOM 1..11
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 12..32
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 33..465
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT CARBOHYD 80
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 118
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 125
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 266
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 403
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 465 AA; 52682 MW; ADC5FCDEDE82A69E CRC64;
MKAAVRSKKS KGSFCHPPLL LLIVAIQFLV IYSPTLDQYM VMLTTGKPGF PSMLIDGRRS
FKQVDEFIPE PHLRCDFRDN RSDVCEMEGA IRILGRTSEV FLVAPSLASI SGGGGGVNAT
GVDANATRWK IQPYTRKGES RVMPGITEVT VRLVTADEAP PCDEWHDVPA IVYSNGGYCG
NYYHDFNDNI IPLFITSRHL AGEVQLLVTQ KQRWWFGKYR EIVEGLTKYE PVDLDAEQRV
RCYRRATVGL HSHKDLSIDP RRAPNNYSMV DFKRFLMWRY ALPREHAIRM EEEDKSKKPR
LLVINRRSRR RFVNLDEIVA AAEGVGFEVA AAELDAHIPA AASAVNSYDA MVAVHGSGLT
NLVFLPMNAV VIQVVPLGRM EGLAMDEYGV PPRDMNMRYL QYNITAEEST LSEVYPRAHP
VFLDPLPIHK QSWSLVKDIY LGQQDVRLDV RRFRPVLLKA LHLLR
