X_BDVV
ID X_BDVV Reviewed; 87 AA.
AC P0DOF0; Q8JJK0; Q912Z9;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2017, sequence version 1.
DT 02-JUN-2021, entry version 11.
DE RecName: Full=X protein;
DE AltName: Full=p10;
GN Name=P/X;
OS Borna disease virus (strain V) (BDV).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Monjiviricetes; Mononegavirales; Bornaviridae; unclassified Bornaviridae.
OX NCBI_TaxID=928296;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
OH NCBI_TaxID=9352; Bradypodidae (three-fingered sloths).
OH NCBI_TaxID=9925; Capra hircus (Goat).
OH NCBI_TaxID=9850; Cervidae (deer).
OH NCBI_TaxID=109474; Crocidura leucodon (Bicoloured white-toothed shrew) (Celebes shrew).
OH NCBI_TaxID=9788; Equidae (horses).
OH NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
OH NCBI_TaxID=56798; Hexaprotodon liberiensis (Pygmy hippopotamus) (Choeropsis liberiensis).
OH NCBI_TaxID=9844; Lama glama (Llama).
OH NCBI_TaxID=9986; Oryctolagus cuniculus (Rabbit).
OH NCBI_TaxID=9940; Ovis aries (Sheep).
OH NCBI_TaxID=8801; Struthio camelus (Common ostrich).
OH NCBI_TaxID=9455; Varecia variegata (Black-and-white ruffed lemur) (Lemur variegatus).
OH NCBI_TaxID=30538; Vicugna pacos (Alpaca) (Lama pacos).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=8183914; DOI=10.1073/pnas.91.10.4362;
RA Briese T., Schneemann A., Lewis A.J., Park Y.-S., Kim S., Ludwig H.,
RA Lipkin W.I.;
RT "Genomic organization of Borna disease virus.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:4362-4366(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=V/FR;
RX PubMed=11602780; DOI=10.1099/0022-1317-82-11-2681;
RA Pleschka S., Staeheli P., Kolodziejek J., Richt J.A., Nowotny N.,
RA Schwemmle M.;
RT "Conservation of coding potential and terminal sequences in four different
RT isolates of Borna disease virus.";
RL J. Gen. Virol. 82:2681-2690(2001).
RN [3]
RP INTERACTION WITH P PROTEIN.
RX PubMed=10708460; DOI=10.1128/jvi.74.7.3413-3417.2000;
RA Malik T.H., Kishi M., Lai P.K.;
RT "Characterization of the P protein-binding domain on the 10-kilodalton
RT protein of Borna disease virus.";
RL J. Virol. 74:3413-3417(2000).
RN [4]
RP MUTAGENESIS OF 2-SER-SER-3; 4-ASP-LEU-5; 6-ARG-LEU-7; 7-LEU--LEU-10;
RP 8-THR-LEU-9; 10-LEU-GLU-11; 12-LEU-GLU-13; 14-ARG-ARG-15; 16-LEU-ASN-17;
RP 18-GLY-ASN-19 AND THR-21.
RC STRAIN=He/80;
RX PubMed=10725419; DOI=10.1099/0022-1317-81-4-939;
RA Wolff T., Pfleger R., Wehner T., Reinhardt J., Richt J.A.;
RT "A short leucine-rich sequence in the Borna disease virus p10 protein
RT mediates association with the viral phospho- and nucleoproteins.";
RL J. Gen. Virol. 81:939-947(2000).
RN [5]
RP REVIEW.
RX PubMed=11815287; DOI=10.2741/a789;
RA Ikuta K., Ibrahim M.S., Kobayashi T., Tomonaga K.;
RT "Borna disease virus and infection in humans.";
RL Front. Biosci. 7:470-495(2002).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=19211764; DOI=10.1128/jvi.02321-08;
RA Poenisch M., Burger N., Staeheli P., Bauer G., Schneider U.;
RT "Protein X of Borna disease virus inhibits apoptosis and promotes viral
RT persistence in the central nervous systems of newborn-infected rats.";
RL J. Virol. 83:4297-4307(2009).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH HOST MAVS.
RX PubMed=23702035; DOI=10.1016/j.biocel.2013.05.012;
RA Li Y., Song W., Wu J., Zhang Q., He J., Li A., Qian J., Zhai A., Hu Y.,
RA Kao W., Wei L., Zhang F., Xu D.;
RT "MAVS-mediated host cell defense is inhibited by Borna disease virus.";
RL Int. J. Biochem. Cell Biol. 45:1546-1555(2013).
RN [8]
RP FUNCTION.
RX PubMed=25333748; DOI=10.1038/ncomms6181;
RA Szelechowski M., Betourne A., Monnet Y., Ferre C.A., Thouard A., Foret C.,
RA Peyrin J.M., Hunot S., Gonzalez-Dunia D.;
RT "A viral peptide that targets mitochondria protects against neuronal
RT degeneration in models of Parkinson's disease.";
RL Nat. Commun. 5:5181-5181(2014).
RN [9]
RP SUBCELLULAR LOCATION, REGION, AND FUNCTION.
RX PubMed=26700735; DOI=10.1096/fj.15-279620;
RA Ferre C.A., Davezac N., Thouard A., Peyrin J.M., Belenguer P., Miquel M.C.,
RA Gonzalez-Dunia D., Szelechowski M.;
RT "Manipulation of the N-terminal sequence of the Borna disease virus X
RT protein improves its mitochondrial targeting and neuroprotective
RT potential.";
RL FASEB J. 30:1523-1533(2016).
CC -!- FUNCTION: Plays an essential role in the inhibition of host apoptosis.
CC Mediates host mitochondria-mediated apoptosis through interaction with
CC the mitochondrial antiviral signaling protein/MAVS and thereby promotes
CC viral persistence in host central nervous system. Within the host
CC nucleus, regulates viral RNA synthesis and polymerase complex assembly.
CC {ECO:0000269|PubMed:19211764, ECO:0000269|PubMed:23702035,
CC ECO:0000269|PubMed:25333748, ECO:0000269|PubMed:26700735}.
CC -!- SUBUNIT: Interacts with P and N proteins. These interactions presumably
CC promote nuclear targeting of the X protein in infected cells
CC (PubMed:10708460). Interacts with host MAVS; this interaction inhibits
CC MAVS-induced apoptosis (PubMed:23702035). {ECO:0000269|PubMed:10708460,
CC ECO:0000269|PubMed:23702035}.
CC -!- SUBCELLULAR LOCATION: Host nucleus. Host mitochondrion
CC {ECO:0000269|PubMed:19211764, ECO:0000269|PubMed:23702035,
CC ECO:0000269|PubMed:26700735}.
CC -!- PTM: Phosphorylated.
CC -!- MISCELLANEOUS: The P/X gene has 2 overlapping open reading frames. One
CC encodes the P protein and the other the X protein. The p24, X and p16
CC proteins are produced by ribosomal leaky scanning.
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DR EMBL; AJ311521; CAC70635.1; -; Genomic_RNA.
DR EMBL; U04608; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR RefSeq; YP_009272535.1; NC_001607.1.
DR GeneID; 28620039; -.
DR KEGG; vg:28620039; -.
DR Proteomes; UP000007804; Genome.
DR Proteomes; UP000124375; Genome.
DR GO; GO:0033650; C:host cell mitochondrion; IDA:UniProtKB.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0019050; P:suppression by virus of host apoptotic process; IDA:UniProtKB.
DR InterPro; IPR009485; BDV_P10.
DR Pfam; PF06515; BDV_P10; 1.
PE 1: Evidence at protein level;
KW Host mitochondrion; Host nucleus; Host-virus interaction;
KW Modulation of host cell apoptosis by virus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..87
FT /note="X protein"
FT /id="PRO_0000079208"
FT REGION 5..16
FT /note="Nuclear export signal"
FT /evidence="ECO:0000269|PubMed:26700735"
FT REGION 18..87
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..48
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 66..80
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 20
FT /note="A -> G (in strain: Isolate CRNP5, Isolate CRP3B and
FT isolate CRP3A)"
FT VARIANT 42
FT /note="T -> I (in strain: Isolate CRNP5, Isolate CRP3B and
FT isolate CRP3A)"
FT VARIANT 50
FT /note="G -> D (in strain: Isolate CRNP5, Isolate CRP3B and
FT isolate CRP3A)"
FT VARIANT 59
FT /note="S -> G (in strain: Isolate CRNP5, Isolate CRP3B and
FT isolate CRP3A)"
FT VARIANT 86
FT /note="V -> I (in strain: Isolate CRNP5, Isolate CRP3B and
FT isolate CRP3A)"
FT MUTAGEN 2..3
FT /note="SS->AA: No effect on interaction with P protein."
FT /evidence="ECO:0000269|PubMed:10725419"
FT MUTAGEN 4..5
FT /note="DL->AA: No effect on interaction with P protein."
FT /evidence="ECO:0000269|PubMed:10725419"
FT MUTAGEN 6..7
FT /note="RL->AA: No effect on interaction with P protein."
FT /evidence="ECO:0000269|PubMed:10725419"
FT MUTAGEN 7..10
FT /note="LTLL->ATAA: Complete loss of interaction with P
FT protein."
FT /evidence="ECO:0000269|PubMed:10725419"
FT MUTAGEN 8..9
FT /note="TL->AA: Complete loss of interaction with P
FT protein."
FT /evidence="ECO:0000269|PubMed:10725419"
FT MUTAGEN 10..11
FT /note="LE->AA: Complete loss of interaction with P
FT protein."
FT /evidence="ECO:0000269|PubMed:10725419"
FT MUTAGEN 12..13
FT /note="LV->AA: Partial loss of interaction with P protein."
FT /evidence="ECO:0000269|PubMed:10725419"
FT MUTAGEN 14..15
FT /note="RR->AA: Complete loss of interaction with P
FT protein."
FT /evidence="ECO:0000269|PubMed:10725419"
FT MUTAGEN 16..17
FT /note="LN->AA: No effect on interaction with P protein."
FT /evidence="ECO:0000269|PubMed:10725419"
FT MUTAGEN 18..19
FT /note="GN->AA: No effect on interaction with P protein."
FT /evidence="ECO:0000269|PubMed:10725419"
FT MUTAGEN 21
FT /note="T->A: No effect on interaction with P protein."
FT /evidence="ECO:0000269|PubMed:10725419"
SQ SEQUENCE 87 AA; 9386 MW; 936743D28F9719FD CRC64;
MSSDLRLTLL ELVRRLNGNA TIESGRLPGG RRRSPDTTTG TTGVTKTTEG PKECIDPTSR
PAPEGPQEEP LHDLRPRPAN RKGAAVE
