ID   CAPP1_MESCR             Reviewed;         966 AA.
AC   P10490; Q6LBL2;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Phosphoenolpyruvate carboxylase 1;
DE            Short=PEPC 1;
DE            Short=PEPCase 1;
DE            EC=4.1.1.31;
GN   Name=PPCA; Synonyms=PPC1;
OS   Mesembryanthemum crystallinum (Common ice plant) (Cryophytum crystallinum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Aizoaceae; Mesembryanthemum;
OC   Mesembryanthemum subgen. Cryophytum.
OX   NCBI_TaxID=3544;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=2710107; DOI=10.1007/bf00427042;
RA   Rickers J., Cushman J., Michalowski C., Schmitt J., Bohnert H.J.;
RT   "Expression of the CAM-form of phospho(enol)pyruvate carboxylase and
RT   nucleotide sequence of a full length cDNA from Mesembryanthemum
RT   crystallinum.";
RL   Mol. Gen. Genet. 215:447-454(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2780306; DOI=10.1093/nar/17.16.6745;
RA   Cushman J.C., Bohnert H.J.;
RT   "Nucleotide sequence of the gene encoding a CAM specific isoform of
RT   phosphoenolpyruvate carboxylase from Mesembryanthemum crystallinum.";
RL   Nucleic Acids Res. 17:6745-6745(1989).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Leaf, and Stem;
RX   PubMed=2535520; DOI=10.2307/3868962;
RA   Cushman J.C., Meyer G., Michalowski C.B., Schmitt J.M., Bohnert H.J.;
RT   "Salt stress leads to differential expression of two isogenes of
RT   phosphoenolpyruvate carboxylase during Crassulacean acid metabolism
RT   induction in the common ice plant.";
RL   Plant Cell 1:715-725(1989).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.
RX   PubMed=1421145; DOI=10.1007/bf00040601;
RA   Cushman J.C., Bohnert H.J.;
RT   "Salt stress alters A/T-rich DNA-binding factor interactions within the
RT   phosphoenolpyruvate carboxylase promoter from Mesembryanthemum
RT   crystallinum.";
RL   Plant Mol. Biol. 20:411-424(1992).
CC   -!- FUNCTION: Through the carboxylation of phosphoenolpyruvate (PEP) it
CC       forms oxaloacetate, a four-carbon dicarboxylic acid source for the
CC       tricarboxylic acid cycle.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- INDUCTION: By salt stress.
CC   -!- MISCELLANEOUS: This isozyme is from salt-induced (CAM) plants.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family. {ECO:0000305}.
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DR   EMBL; X13660; CAA31956.1; -; mRNA.
DR   EMBL; X14587; CAA32727.1; -; Genomic_DNA.
DR   EMBL; X63774; CAA45309.1; -; Genomic_DNA.
DR   PIR; S05506; QYIX1.
DR   AlphaFoldDB; P10490; -.
DR   SMR; P10490; -.
DR   PRIDE; P10490; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PTHR30523; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; SSF51621; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   2: Evidence at transcript level;
KW   Allosteric enzyme; Carbon dioxide fixation; Cytoplasm; Lyase; Magnesium;
KW   Phosphoprotein; Photosynthesis; Stress response.
FT   CHAIN           1..966
FT                   /note="Phosphoenolpyruvate carboxylase 1"
FT                   /id="PRO_0000166670"
FT   ACT_SITE        173
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        601
FT                   /evidence="ECO:0000250"
FT   MOD_RES         11
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   966 AA;  110660 MW;  4B49C9DDB2FAD360 CRC64;
     MSTVKLDRLT SIDAQLRLLA PKKVSEDDKL IEYDALLLDR FLDILQNLHG EDIKETVQEL
     YEQSAEYERT HDPKKLEELG SMVTSLDAGD SIVVAKSFSH MLNLANLAEE VQISRRKRVK
     KVKKGDFMDE NTAMTESDME ETLRRLIVDL KKSPQEIFET LKNQTVDLVF TAHPTQSVRR
     SLLQKHGRIR DCLAQLYAKD ITPDDKQELD EALQREIQAA FRTDEIRRTQ PTPQDEMRAG
     MSYFHETIWN GVPKFLRRLD TALKNIGITE RVPYNAPLIQ FSSWMGGDRD GNPRVTPEVT
     RDVCLLARMM AANMYFSQID ELMFELSMWR CTDELRERAE ELHKYSKRDS KHYIEFWKQI
     PSSEPYRVIL ADVRDKLYYT RERSRQLLAS EVSEIPVEAT FTEIDQFLEP LELCYRSLCA
     CGDRPVADGS LLDFMRQVAT FGLCLVKLDI RQESERHTDV MDAITTHLGI GSYRDWTEEK
     RQDWLLSELR GKRPLFGPDL PRTDEIADVL DTINVIAELP SDSFGAYVIS MATAPSDVLA
     VELLQRECKV KKPLRVVPLF EKLADLEAAP ASMTRLFSVD WYKNRIDGKQ EVMIGYSDSG
     KDAGRLSAAW QMYKVQEELL KVSKKFGVKL TMFHGRGGTV GRGGGPTHLA ILAQPAETIG
     GSLRVTIQGE VIEQSFGEQH LCFRTLQRYT AATLEHGMNP PKSPKPEWRA LLDQMAVVAT
     EEYRSIVFKE PRFVEYFRLA TPETEYGRMN IGSRPSKRKP SGGIESLRAI PWIFAWTQTR
     FHLPVWLGVG GALKHVLEKD IRNHNMLRDM YNNFPFFRVT IDLLEMVFAK GDPEIAALYD
     KLLVSEELQS FGERLRANYE DTKRRLLEVA GHKDLLEGDP YLKQRLRLRD PYITTLNVCQ
     AYTLKRIRDP DFKVTERPHL SKEIMDAHKA AAELVKLNPT SEYAPGLEDT LILTMKGVAA
     GLQNTG