Y0081_MYCTU
ID Y0081_MYCTU Reviewed; 114 AA.
AC P9WMI7; L0T5M3; O53626; Q7DAH4;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 42.
DE RecName: Full=Uncharacterized HTH-type transcriptional regulator Rv0081;
GN OrderedLocusNames=Rv0081;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP INDUCTION BY HYPOXIA.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=11416222; DOI=10.1073/pnas.121172498;
RA Sherman D.R., Voskuil M., Schnappinger D., Liao R., Harrell M.I.,
RA Schoolnik G.K.;
RT "Regulation of the Mycobacterium tuberculosis hypoxic response gene
RT encoding alpha -crystallin.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:7534-7539(2001).
RN [3]
RP INDUCTION BY NITRIC OXIDE (NO) AND BY HYPOXIA, AND DORMANCY REGULON.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=12953092; DOI=10.1084/jem.20030205;
RA Voskuil M.I., Schnappinger D., Visconti K.C., Harrell M.I., Dolganov G.M.,
RA Sherman D.R., Schoolnik G.K.;
RT "Inhibition of respiration by nitric oxide induces a Mycobacterium
RT tuberculosis dormancy program.";
RL J. Exp. Med. 198:705-713(2003).
RN [4]
RP INDUCTION BY CARBON MONOXIDE (CO).
RC STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX PubMed=18474359; DOI=10.1016/j.chom.2008.03.007;
RA Shiloh M.U., Manzanillo P., Cox J.S.;
RT "Mycobacterium tuberculosis senses host-derived carbon monoxide during
RT macrophage infection.";
RL Cell Host Microbe 3:323-330(2008).
RN [5]
RP INDUCTION BY CARBON MONOXIDE (CO), AND DORMANCY REGULON.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=18400743; DOI=10.1074/jbc.m802274200;
RA Kumar A., Deshane J.S., Crossman D.K., Bolisetty S., Yan B.S., Kramnik I.,
RA Agarwal A., Steyn A.J.;
RT "Heme oxygenase-1-derived carbon monoxide induces the Mycobacterium
RT tuberculosis dormancy regulon.";
RL J. Biol. Chem. 283:18032-18039(2008).
RN [6]
RP BIOTECHNOLOGY.
RX PubMed=19553548; DOI=10.1128/cvi.00111-09;
RA Black G.F., Thiel B.A., Ota M.O., Parida S.K., Adegbola R., Boom W.H.,
RA Dockrell H.M., Franken K.L., Friggen A.H., Hill P.C., Klein M.R.,
RA Lalor M.K., Mayanja H., Schoolnik G., Stanley K., Weldingh K.,
RA Kaufmann S.H., Walzl G., Ottenhoff T.H.;
RT "Immunogenicity of novel DosR regulon-encoded candidate antigens of
RT Mycobacterium tuberculosis in three high-burden populations in Africa.";
RL Clin. Vaccine Immunol. 16:1203-1212(2009).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- INDUCTION: A member of the dormancy regulon. Induced in response to
CC reduced oxygen tension (hypoxia), low levels of nitric oxide (NO) and
CC carbon monoxide (CO). It is hoped that this regulon will give insight
CC into the latent, or dormant phase of infection.
CC {ECO:0000269|PubMed:11416222, ECO:0000269|PubMed:12953092,
CC ECO:0000269|PubMed:18400743, ECO:0000269|PubMed:18474359}.
CC -!- BIOTECHNOLOGY: This protein serves as an immunogenic antigen, inducing
CC gamma-interferon responses in whole-blood cultures from M.tuberculosis-
CC exposed adults in Uganda, The Gambia and South Africa, indicating this
CC might be a good vaccine candidate. {ECO:0000269|PubMed:19553548}.
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DR EMBL; AL123456; CCP42806.1; -; Genomic_DNA.
DR PIR; B70850; B70850.
DR RefSeq; NP_214595.1; NC_000962.3.
DR RefSeq; WP_003400657.1; NZ_NVQJ01000005.1.
DR PDB; 6JMI; X-ray; 2.90 A; A/B/C/D=2-114.
DR PDBsum; 6JMI; -.
DR AlphaFoldDB; P9WMI7; -.
DR SMR; P9WMI7; -.
DR STRING; 83332.Rv0081; -.
DR PaxDb; P9WMI7; -.
DR DNASU; 887012; -.
DR GeneID; 45424045; -.
DR GeneID; 887012; -.
DR KEGG; mtu:Rv0081; -.
DR TubercuList; Rv0081; -.
DR eggNOG; COG0640; Bacteria.
DR OMA; IWECLGL; -.
DR PhylomeDB; P9WMI7; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR CDD; cd00090; HTH_ARSR; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011991; ArsR-like_HTH.
DR InterPro; IPR001845; HTH_ArsR_DNA-bd_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PRINTS; PR00778; HTHARSR.
DR SMART; SM00418; HTH_ARSR; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50987; HTH_ARSR_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..114
FT /note="Uncharacterized HTH-type transcriptional regulator
FT Rv0081"
FT /id="PRO_0000392681"
FT DOMAIN 2..97
FT /note="HTH arsR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT DNA_BIND 37..60
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT HELIX 5..16
FT /evidence="ECO:0007829|PDB:6JMI"
FT HELIX 20..31
FT /evidence="ECO:0007829|PDB:6JMI"
FT HELIX 36..40
FT /evidence="ECO:0007829|PDB:6JMI"
FT HELIX 48..60
FT /evidence="ECO:0007829|PDB:6JMI"
FT STRAND 63..79
FT /evidence="ECO:0007829|PDB:6JMI"
FT HELIX 81..101
FT /evidence="ECO:0007829|PDB:6JMI"
SQ SEQUENCE 114 AA; 12356 MW; AF3C88714DCC1B4F CRC64;
MESEPLYKLK AEFFKTLAHP ARIRILELLV ERDRSVGELL SSDVGLESSN LSQQLGVLRR
AGVVAARRDG NAMIYSIAAP DIAELLAVAR KVLARVLSDR VAVLEDLRAG GSAT
