CAPP4_ARATH
ID CAPP4_ARATH Reviewed; 1032 AA.
AC Q8GVE8; Q9CA39; Q9SX35;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Phosphoenolpyruvate carboxylase 4;
DE Short=AtPPC4;
DE Short=PEPC 4;
DE Short=PEPCase 4;
DE EC=4.1.1.31;
GN Name=PPC4; OrderedLocusNames=At1g68750; ORFNames=F14K14.14, F24J5.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=12805623; DOI=10.1104/pp.102.019653;
RA Sanchez R., Cejudo F.J.;
RT "Identification and expression analysis of a gene encoding a bacterial-type
RT phosphoenolpyruvate carboxylase from Arabidopsis and rice.";
RL Plant Physiol. 132:949-957(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: Through the carboxylation of phosphoenolpyruvate (PEP) it
CC forms oxaloacetate, a four-carbon dicarboxylic acid source for the
CC tricarboxylic acid cycle.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in flowers and siliques,
CC and detectable in roots. {ECO:0000269|PubMed:12805623}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD49968.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAG52040.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AJ532903; CAD58727.1; -; mRNA.
DR EMBL; AC008075; AAD49968.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC011914; AAG52040.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34835.1; -; Genomic_DNA.
DR PIR; C96712; C96712.
DR RefSeq; NP_177043.2; NM_105548.5.
DR AlphaFoldDB; Q8GVE8; -.
DR SMR; Q8GVE8; -.
DR BioGRID; 28427; 6.
DR STRING; 3702.AT1G68750.1; -.
DR iPTMnet; Q8GVE8; -.
DR PaxDb; Q8GVE8; -.
DR PRIDE; Q8GVE8; -.
DR ProteomicsDB; 240315; -.
DR EnsemblPlants; AT1G68750.1; AT1G68750.1; AT1G68750.
DR GeneID; 843206; -.
DR Gramene; AT1G68750.1; AT1G68750.1; AT1G68750.
DR KEGG; ath:AT1G68750; -.
DR Araport; AT1G68750; -.
DR TAIR; locus:2012448; AT1G68750.
DR eggNOG; ENOG502QPVS; Eukaryota.
DR HOGENOM; CLU_006557_2_0_1; -.
DR InParanoid; Q8GVE8; -.
DR OMA; PCEYDPL; -.
DR OrthoDB; 775417at2759; -.
DR PhylomeDB; Q8GVE8; -.
DR BioCyc; ARA:AT1G68750-MON; -.
DR BRENDA; 4.1.1.31; 399.
DR PRO; PR:Q8GVE8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8GVE8; baseline and differential.
DR Genevisible; Q8GVE8; AT.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; ISS:TAIR.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-KW.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; ISS:TAIR.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PTHR30523; 1.
DR Pfam; PF00311; PEPcase; 2.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; SSF51621; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 2: Evidence at transcript level;
KW Carbon dioxide fixation; Cytoplasm; Lyase; Magnesium; Photosynthesis;
KW Reference proteome.
FT CHAIN 1..1032
FT /note="Phosphoenolpyruvate carboxylase 4"
FT /id="PRO_0000166660"
FT REGION 377..407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 154
FT /evidence="ECO:0000250"
FT ACT_SITE 699
FT /evidence="ECO:0000250"
SQ SEQUENCE 1032 AA; 116586 MW; 750116B15B9ECA3E CRC64;
MTDTTDDIAE EISFQSFEDD CKLLGSLFHD VLQREVGNPF MEKVERIRIL AQSALNLRMA
GIEDTANLLE KQLTSEISKM PLEEALTLAR TFTHSLNLMG IADTHHRMHK VHNVTQLARS
CDDIFSQLLQ SGISPDELYK TVCKQEVEIV LTAHPTQINR RTLQYKHIRI AHLLEYNTRS
DLSVEDRETL IEDLVREITS LWQTDELRRQ KPTPVDEARA GLNIVEQSLW KAVPQYLRRV
SNSLKKFTGK PLPLTCTPMK FGSWMGGDRD GNPNVTAKVT KEVSLLSRWM AIDLYIREVD
SLRFELSTDR CSDRFSRLAD KILEKDYDRG KSNFQKQQSS SCLPTQLPAR AHLPACIDFG
ESRHTKFEIA TTDYMPPNLQ KQNEQDFSES DWEKIDNGSR SGLTSRGSFS STSQLLLQRK
LFEESQVGKT SFQKLLEPPP LKRAGSAPYR IVLGEVKEKL VKTRRLLELL IEGLPCEYDP
KNSYETSDQL LEPLLLCYES LQSSGARVLA DGRLADLIRR VSTFGMVLVK LDLRQEAARH
SEALDAITTY LDMGTYSEWD EEKKLEFLTR ELKGKRPLVP QCIKVGPDVK EVLDTFRVAA
ELGSESLGAY VISMASNASD VLAVELLQKD ARLALTSEHG KPCPGGTLRV VPLFETVNDL
RAAGPSIRKL LSIDWYREHI QKNHNGHQEV MVGYSDSGKD AGRFTAAWEL YKAQENVVAA
CNEFGIKITL FHGRGGSIGR GGGPTYLAIQ SQPPGSVMGS LRSTEQGEMV QAKFGIPQTA
VRQLEVYTTA VLLATLKPPQ PPREEKWRNL MEEISGISCQ HYRSTVYENP EFLSYFHEAT
PQAELGFLNI GSRPTRRKSS SGIGHLRAIP WVFAWTQTRF VLPAWLGVGA GLKGVSEKGH
ADDLKEMYKE WPFFQSTLEL IEMVLAKADI PMTKHYDEQL VSEKRRGLGT ELRKELMTTE
KYVLVISGHE KLLQDNKSLK KLIDSRLPYL NAMNMLQVEI LKRLRRDEDN NKLRDALLIT
INGIAAGMRN TG
