CAPPA_METBF
ID CAPPA_METBF Reviewed; 526 AA.
AC Q469A3;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_01904};
DE Short=PEPC {ECO:0000255|HAMAP-Rule:MF_01904};
DE Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_01904};
DE EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_01904};
GN Name=ppcA {ECO:0000255|HAMAP-Rule:MF_01904}; OrderedLocusNames=Mbar_A2632;
OS Methanosarcina barkeri (strain Fusaro / DSM 804).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX NCBI_TaxID=269797;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Fusaro / DSM 804;
RX PubMed=16980466; DOI=10.1128/jb.00810-06;
RA Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S.,
RA Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.;
RT "The Methanosarcina barkeri genome: comparative analysis with
RT Methanosarcina acetivorans and Methanosarcina mazei reveals extensive
RT rearrangement within methanosarcinal genomes.";
RL J. Bacteriol. 188:7922-7931(2006).
CC -!- FUNCTION: Catalyzes the irreversible beta-carboxylation of
CC phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon
CC dicarboxylic acid source for the tricarboxylic acid cycle.
CC {ECO:0000255|HAMAP-Rule:MF_01904}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01904};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01904};
CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_01904}.
CC -!- SIMILARITY: Belongs to the PEPCase type 2 family. {ECO:0000255|HAMAP-
CC Rule:MF_01904}.
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DR EMBL; CP000099; AAZ71539.1; -; Genomic_DNA.
DR RefSeq; WP_011307580.1; NC_007355.1.
DR AlphaFoldDB; Q469A3; -.
DR SMR; Q469A3; -.
DR STRING; 269797.Mbar_A2632; -.
DR EnsemblBacteria; AAZ71539; AAZ71539; Mbar_A2632.
DR GeneID; 3625651; -.
DR KEGG; mba:Mbar_A2632; -.
DR eggNOG; arCOG04435; Archaea.
DR HOGENOM; CLU_517433_0_0_2; -.
DR OMA; QSAFRYD; -.
DR OrthoDB; 23334at2157; -.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR HAMAP; MF_01904; PEPcase_type2; 1.
DR InterPro; IPR007566; PEP_COase_arc-type.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR Pfam; PF14010; PEPcase_2; 1.
DR PIRSF; PIRSF006677; UCP006677; 1.
DR SUPFAM; SSF51621; SSF51621; 1.
DR TIGRFAMs; TIGR02751; PEPCase_arch; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation; Lyase; Magnesium.
FT CHAIN 1..526
FT /note="Phosphoenolpyruvate carboxylase"
FT /id="PRO_0000309603"
SQ SEQUENCE 526 AA; 58068 MW; A08490FAE19B6E42 CRC64;
MSRKTVFPKV MCTQHPDSAS KYIATQEEPG EAIEAAQIFG CDEYMPDYEG KATPYHQNVQ
VVSKFIEETD LIPGKDIIIT PRAPSAVQEN QFRQLMVMMS IAEANYNALE YSDVQAINEF
VHPMTDSVRE IIGAQQHMVD VSELAKKEFG FSMEVPCIIP LIEDAPALLH AKELAENTLF
AWKGHFGTVP DKFRVFLGKS DSALSFGHVA STLSCKYAIN GICELNSELD TQTGIIFGAG
TLPFRGHLDL MNAENFFKEY RGVGTITLQS ALRYSHKKGD AESLVKLAKA RLPETPELFS
AEEKEEIINL IGIFGASYSR IIRQLAPTIN RIADLLPQQR DRLMHKGTGG YSRSAPDISG
LVNLCRTDIG KELEASMPAE DLQLPRAIKF TGALYSIGLP PEFIGTGRAL EEAREKLGEA
ACENLLTKYF PSLAGDLNFA SEYLDLNVAS RFLSSECLKE VSKDLDILHG AFALETSPEP
SYRILVEMMQ PDLLQAGSRG NCMDEEVSQL VCSTLTKMGK IRKALG
