ID   CAPPA_METHJ             Reviewed;         484 AA.
AC   Q2FLH1;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   03-MAY-2011, sequence version 2.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_01904};
DE            Short=PEPC {ECO:0000255|HAMAP-Rule:MF_01904};
DE            Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_01904};
DE            EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_01904};
GN   Name=ppcA {ECO:0000255|HAMAP-Rule:MF_01904}; OrderedLocusNames=Mhun_0174;
OS   Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 /
OS   JF-1).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanomicrobiales; Methanospirillaceae; Methanospirillum.
OX   NCBI_TaxID=323259;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27890 / DSM 864 / NBRC 100397 / JF-1;
RX   PubMed=26744606; DOI=10.1186/s40793-015-0124-8;
RA   Gunsalus R.P., Cook L.E., Crable B., Rohlin L., McDonald E., Mouttaki H.,
RA   Sieber J.R., Poweleit N., Zhou H., Lapidus A.L., Daligault H.E., Land M.,
RA   Gilna P., Ivanova N., Kyrpides N., Culley D.E., McInerney M.J.;
RT   "Complete genome sequence of Methanospirillum hungatei type strain JF1.";
RL   Stand. Genomic Sci. 11:2-2(2016).
CC   -!- FUNCTION: Catalyzes the irreversible beta-carboxylation of
CC       phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon
CC       dicarboxylic acid source for the tricarboxylic acid cycle.
CC       {ECO:0000255|HAMAP-Rule:MF_01904}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01904};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01904};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_01904}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01904}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABD39949.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; CP000254; ABD39949.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; Q2FLH1; -.
DR   SMR; Q2FLH1; -.
DR   STRING; 323259.Mhun_0174; -.
DR   EnsemblBacteria; ABD39949; ABD39949; Mhun_0174.
DR   KEGG; mhu:Mhun_0174; -.
DR   eggNOG; arCOG04435; Archaea.
DR   HOGENOM; CLU_517433_0_0_2; -.
DR   Proteomes; UP000001941; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_01904; PEPcase_type2; 1.
DR   InterPro; IPR007566; PEP_COase_arc-type.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   Pfam; PF14010; PEPcase_2; 1.
DR   PIRSF; PIRSF006677; UCP006677; 1.
DR   SUPFAM; SSF51621; SSF51621; 1.
DR   TIGRFAMs; TIGR02751; PEPCase_arch; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation; Lyase; Magnesium; Reference proteome.
FT   CHAIN           1..484
FT                   /note="Phosphoenolpyruvate carboxylase"
FT                   /id="PRO_0000309605"
SQ   SEQUENCE   484 AA;  55411 MW;  9EAF45C89E40AC3B CRC64;
     MKIPRTMSTQ HPDNVHTPFF TENIELTGED EVKEAYYVYS HLGCTEQMWD CEGKEVDNYV
     VKKLLSRYGD YFKEHHLGRD LFLTLRVPNP EIERTEAKIL LETLGSIPRS YDVAHHFYND
     TYAPIFEIIL PMTTSFASID NIYQYYCDFV IGQQYKRLGG RDLTIAEWIG PFHPDKIRVI
     PLFEDKDGML AADTILRRYF QDKDLDYQRV FLARSDPAVN YGQIGAVLLN KIALWRLHLL
     SEDTGIPVYP IIGAGSAPFR GNLRPDTVRR VTDEYAGAYT FTIQSAFKYD TNLEEAVSAI
     RYLEEREITP AREIDDTYAI SLIERYAAGY QKQIRSLAPL INRVAAYIPS RRKRKLHVGL
     FGYSRNMGGV SLPRAITVTA ALYSIGIPPE VLGLSALTEN DREFVLENYR YVTEDLSDAC
     RYLNPDSSFL PDEIKKILPD WIEIDPHSEH RVLSGQIEKA VTACQIDSVQ DMIIRAGAIR
     KFLG