ID   CAPPA_METKA             Reviewed;         532 AA.
AC   Q8TYV1;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_01904};
DE            Short=PEPC {ECO:0000255|HAMAP-Rule:MF_01904};
DE            Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_01904};
DE            EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_01904};
GN   Name=ppcA {ECO:0000255|HAMAP-Rule:MF_01904}; OrderedLocusNames=MK0190;
OS   Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938).
OC   Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae;
OC   Methanopyrus.
OX   NCBI_TaxID=190192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
RX   PubMed=11930014; DOI=10.1073/pnas.032671499;
RA   Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N.,
RA   Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A.,
RA   Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G.,
RA   Koonin E.V., Kozyavkin S.A.;
RT   "The complete genome of hyperthermophile Methanopyrus kandleri AV19 and
RT   monophyly of archaeal methanogens.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).
CC   -!- FUNCTION: Catalyzes the irreversible beta-carboxylation of
CC       phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon
CC       dicarboxylic acid source for the tricarboxylic acid cycle.
CC       {ECO:0000255|HAMAP-Rule:MF_01904}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01904};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01904};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_01904}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01904}.
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DR   EMBL; AE009439; AAM01407.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8TYV1; -.
DR   SMR; Q8TYV1; -.
DR   STRING; 190192.MK0190; -.
DR   EnsemblBacteria; AAM01407; AAM01407; MK0190.
DR   KEGG; mka:MK0190; -.
DR   PATRIC; fig|190192.8.peg.190; -.
DR   HOGENOM; CLU_517433_0_0_2; -.
DR   OMA; QSAFRYD; -.
DR   Proteomes; UP000001826; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_01904; PEPcase_type2; 1.
DR   InterPro; IPR007566; PEP_COase_arc-type.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   Pfam; PF14010; PEPcase_2; 1.
DR   PIRSF; PIRSF006677; UCP006677; 1.
DR   SUPFAM; SSF51621; SSF51621; 1.
DR   TIGRFAMs; TIGR02751; PEPCase_arch; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation; Lyase; Magnesium; Reference proteome.
FT   CHAIN           1..532
FT                   /note="Phosphoenolpyruvate carboxylase"
FT                   /id="PRO_0000309601"
SQ   SEQUENCE   532 AA;  59778 MW;  98E64A0E55D089C9 CRC64;
     MEKVREARIA RTLATQHPDA TKFVRVQEEP EEAVECLVEL GAEEYMVDFE GKLTPYLQPI
     QVLMELYDAG VRVGEERFVI VRVPSATKEN VLRQVQALLG VMEANSELLK EDPDARGIFE
     VVHPMTSSPE ELVETVDRIS YARRFASREL DVPLKAGNLR IIPLIEEVPE LLDIRNILTG
     YVEGMREIGM DVSYLRVFIG RSDPALSYGH LPAVLACKLA IFEVYELSDE LGVPMAPILG
     GGCLPFRGHI RPGMEEEFVE EYAGTATYTV QSGFRYDHDR EKAVASIRRI NELAANDPLQ
     LSGDDIEYLV LATAIFMKHY LSVFFRCIGT LNIVADMIPN TRDRLARKGP VGYARDIPEP
     DRVGAQCKDL GDVGRELYRE LRRMRVEKLP ELPRAIKFTG ACYTVGMPPE LIGTGRGLAE
     IEERLGEDAL DAVISRLYPM LREDLQFAVE YTFLETAGSV LPSSGVAMVN TDLEYCVEYL
     DLEPPSDFEY QNLVHTLEPY LRYVVSEGGV EEVNPFVRDL LLEMGRMRGS LG