ID   CAPPA_METMA             Reviewed;         526 AA.
AC   Q8PS70;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_01904};
DE            Short=PEPC {ECO:0000255|HAMAP-Rule:MF_01904};
DE            Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_01904};
DE            EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_01904};
GN   Name=ppcA {ECO:0000255|HAMAP-Rule:MF_01904}; OrderedLocusNames=MM_3212;
OS   Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM
OS   11833 / OCM 88) (Methanosarcina frisia).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=192952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88;
RX   PubMed=12125824;
RA   Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA   Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA   Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA   Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA   Fritz H.-J., Gottschalk G.;
RT   "The genome of Methanosarcina mazei: evidence for lateral gene transfer
RT   between Bacteria and Archaea.";
RL   J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
CC   -!- FUNCTION: Catalyzes the irreversible beta-carboxylation of
CC       phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon
CC       dicarboxylic acid source for the tricarboxylic acid cycle.
CC       {ECO:0000255|HAMAP-Rule:MF_01904}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01904};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01904};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_01904}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01904}.
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DR   EMBL; AE008384; AAM32908.1; -; Genomic_DNA.
DR   RefSeq; WP_011035107.1; NC_003901.1.
DR   AlphaFoldDB; Q8PS70; -.
DR   SMR; Q8PS70; -.
DR   STRING; 192952.MM_3212; -.
DR   EnsemblBacteria; AAM32908; AAM32908; MM_3212.
DR   GeneID; 44087031; -.
DR   GeneID; 66135773; -.
DR   KEGG; mma:MM_3212; -.
DR   PATRIC; fig|192952.21.peg.3732; -.
DR   eggNOG; arCOG04435; Archaea.
DR   HOGENOM; CLU_517433_0_0_2; -.
DR   OMA; QSAFRYD; -.
DR   Proteomes; UP000000595; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_01904; PEPcase_type2; 1.
DR   InterPro; IPR007566; PEP_COase_arc-type.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   Pfam; PF14010; PEPcase_2; 1.
DR   PIRSF; PIRSF006677; UCP006677; 1.
DR   SUPFAM; SSF51621; SSF51621; 1.
DR   TIGRFAMs; TIGR02751; PEPCase_arch; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation; Lyase; Magnesium; Reference proteome.
FT   CHAIN           1..526
FT                   /note="Phosphoenolpyruvate carboxylase"
FT                   /id="PRO_0000309604"
SQ   SEQUENCE   526 AA;  58177 MW;  7CCCE37156EF8B55 CRC64;
     MSKKATYPKV MCTQHPDSAS KYISTQEEPG EAIEAAVVFG CDEYMPDYEG KATPYHQNVQ
     IVSRFIEETD LIPGKDIFIT PRAPSAAQEN RFRQLMVMMS IAEANHGALE YSDVQAINEF
     VHPMTGTVGE ILDAQQHMVD VGELAKKEFG VAMEVPRIIP LIEDAPALLH AKELAENTLL
     SWEKRFGAAP EKFRVFLGKS DSALSFGHVA STLSCKYAIS GISELDLELD TSTGIIFGAG
     TLPFRGHLSL KNAENFFREY RGIGTITLQS AVRYSHDKGE AEALVRLAEE RLPESPEIYS
     GEEKEEIVNL IGIFGARYNR IIREMSCTIN QLAGLLPQQR DRLMHKGTGG YSRNVPDISG
     LVCLCRKDVG KELSASMPAE DLNLPRAIKF TGALYSIGLP PEFIGTGTAL EEAREKLGEE
     ACERLLKKYF PSLASDLSFA SGYLDLNVAS RFLPEACLKE IRKDIEVLRD TFSLKVQPEP
     SYRILLEMMQ PDLLQAGTKG NCMDEEVSQL VCSTLTKMGK IRKALG