CAPPA_METS5
ID CAPPA_METS5 Reviewed; 509 AA.
AC A4YES9;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 1.
DT 25-MAY-2022, entry version 73.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_01904};
DE Short=PEPC {ECO:0000255|HAMAP-Rule:MF_01904};
DE Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_01904};
DE EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_01904};
GN Name=ppcA {ECO:0000255|HAMAP-Rule:MF_01904}; OrderedLocusNames=Msed_0756;
OS Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509
OS / TH2).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Metallosphaera.
OX NCBI_TaxID=399549;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2;
RX PubMed=18083856; DOI=10.1128/aem.02019-07;
RA Auernik K.S., Maezato Y., Blum P.H., Kelly R.M.;
RT "The genome sequence of the metal-mobilizing, extremely thermoacidophilic
RT archaeon Metallosphaera sedula provides insights into bioleaching-
RT associated metabolism.";
RL Appl. Environ. Microbiol. 74:682-692(2008).
CC -!- FUNCTION: Catalyzes the irreversible beta-carboxylation of
CC phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon
CC dicarboxylic acid source for the tricarboxylic acid cycle.
CC {ECO:0000255|HAMAP-Rule:MF_01904}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01904};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01904};
CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_01904}.
CC -!- SIMILARITY: Belongs to the PEPCase type 2 family. {ECO:0000255|HAMAP-
CC Rule:MF_01904}.
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DR EMBL; CP000682; ABP94931.1; -; Genomic_DNA.
DR RefSeq; WP_012020718.1; NC_009440.1.
DR AlphaFoldDB; A4YES9; -.
DR SMR; A4YES9; -.
DR STRING; 399549.Msed_0756; -.
DR EnsemblBacteria; ABP94931; ABP94931; Msed_0756.
DR GeneID; 5103445; -.
DR KEGG; mse:Msed_0756; -.
DR eggNOG; arCOG04435; Archaea.
DR HOGENOM; CLU_517433_0_0_2; -.
DR OMA; QSAFRYD; -.
DR Proteomes; UP000000242; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR HAMAP; MF_01904; PEPcase_type2; 1.
DR InterPro; IPR007566; PEP_COase_arc-type.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR Pfam; PF14010; PEPcase_2; 1.
DR PIRSF; PIRSF006677; UCP006677; 1.
DR SUPFAM; SSF51621; SSF51621; 1.
DR TIGRFAMs; TIGR02751; PEPCase_arch; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation; Lyase; Magnesium; Reference proteome.
FT CHAIN 1..509
FT /note="Phosphoenolpyruvate carboxylase"
FT /id="PRO_1000088478"
SQ SEQUENCE 509 AA; 58245 MW; FCF967A96862D3C5 CRC64;
MRPIPRTMST QHPDNATVPE WAKGDVIEGE AEVIEAYYAF SRLNVHEVMW DAEGKDVDTH
VVRKLFSSFD EYFKNNILGE DIFLTYRLPN PKIEGAERKV FAETMESIPI TFDVAERFYG
RKVVPVFEVI LPFTTNASDI ISVARYYERA VAMEENIELQ DGVYVRDLVG EIYPKRIEVI
PLIEDKDSLL NTRNIIEGYY RAIKPSYMRL FIARSDPAMN YGMLTAVLLA KYALSEAGKL
AEELGIPIFP IIGVGSLPFR GHLSPENYQR VMEEYEGVYT FTIQSAFKYD YSEEQVKGAI
SHINREEVKE PRILGEEEKK VTRDIIETYT LSYQPVIESL ANLINTVALH LPRRRARKLH
ISLFGYARST GKVMLPRAIT FVGSLYSVGL PPEVIGISSL GKLNEMQWNI LEENYKFLKN
DLQKASEFIN PEGLSTLVSY GYLDAEISKK LEEDIKYLES MGVKIGPRSY ETKKHALLSQ
LLMLSLKEKK YNEVKQYARE MAVIRKSIG
