ID   CAPPA_PYRHO             Reviewed;         469 AA.
AC   O57764;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 2.
DT   25-MAY-2022, entry version 101.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_01904};
DE            Short=PEPC {ECO:0000255|HAMAP-Rule:MF_01904};
DE            Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_01904};
DE            EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_01904};
GN   Name=ppcA {ECO:0000255|HAMAP-Rule:MF_01904}; OrderedLocusNames=PH0016;
OS   Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS   100139 / OT-3).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=70601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX   PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA   Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA   Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA   Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA   Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- FUNCTION: Catalyzes the irreversible beta-carboxylation of
CC       phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon
CC       dicarboxylic acid source for the tricarboxylic acid cycle.
CC       {ECO:0000255|HAMAP-Rule:MF_01904}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01904};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01904};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_01904}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01904}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA29084.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; BA000001; BAA29084.1; ALT_INIT; Genomic_DNA.
DR   PIR; E71219; E71219.
DR   RefSeq; WP_048053011.1; NC_000961.1.
DR   AlphaFoldDB; O57764; -.
DR   SMR; O57764; -.
DR   STRING; 70601.3256401; -.
DR   DNASU; 1443918; -.
DR   EnsemblBacteria; BAA29084; BAA29084; BAA29084.
DR   GeneID; 1443918; -.
DR   KEGG; pho:PH0016; -.
DR   eggNOG; arCOG04435; Archaea.
DR   OrthoDB; 23334at2157; -.
DR   Proteomes; UP000000752; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_01904; PEPcase_type2; 1.
DR   InterPro; IPR007566; PEP_COase_arc-type.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   Pfam; PF14010; PEPcase_2; 1.
DR   PIRSF; PIRSF006677; UCP006677; 1.
DR   SUPFAM; SSF51621; SSF51621; 1.
DR   TIGRFAMs; TIGR02751; PEPCase_arch; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation; Lyase; Magnesium.
FT   CHAIN           1..469
FT                   /note="Phosphoenolpyruvate carboxylase"
FT                   /id="PRO_0000309613"
SQ   SEQUENCE   469 AA;  53953 MW;  8244B4BEF29D2D74 CRC64;
     MIPRIMSTQH PDNYSIPFFA SSPILGGEDE ITEAFYAFSV LGADEQMWDF EGKEVDEFVV
     KKLLERYPTF FKENILGEDL RLTPRVPNPS VEKAEAKLLL ETLQGITRAA DYARVFYLDN
     IAPIFEVILP MTTSLAEMLR VHELYRKIVS LSGEVIYDVT VKEWIGEFYP KEINVIPLFE
     TKVALLKSGR IIREYIGRKR PEYLRVFFAR SDPAMNYGLL SAVTYVKKAL EQVGEVEEET
     STPIYPIIGV GSPPFRGGMR PENVKKVLKE YPSVQTYTIQ SSFKYDHPTK DVVKAVEIVK
     SKKREAPDPL DIPEFIQLYE IEYQKQLKIL APYIRNIVTR IPDRRKRKLH IGLFGYSRNV
     GGLSLPRVIK FTASLYSIGL PPELLGLNEL SDNQLDSISE YYKNLYDDLE FAMRFFSFKV
     AERVGLRELS EKVKEFKPDI DDEYVREAEL VFRGQGDVMK LAQIRGFLG