CAPPA_PYRIL
ID CAPPA_PYRIL Reviewed; 461 AA.
AC A1RR50;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 25-MAY-2022, entry version 71.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_01904};
DE Short=PEPC {ECO:0000255|HAMAP-Rule:MF_01904};
DE Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_01904};
DE EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_01904};
GN Name=ppcA {ECO:0000255|HAMAP-Rule:MF_01904}; OrderedLocusNames=Pisl_0252;
OS Pyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3).
OC Archaea; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae;
OC Pyrobaculum.
OX NCBI_TaxID=384616;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 4184 / JCM 9189 / GEO3;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T.,
RA Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Mikhailova N., Lowe T., Richardson P.;
RT "Complete sequence of Pyrobaculum islandicum DSM 4184.";
RL Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the irreversible beta-carboxylation of
CC phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon
CC dicarboxylic acid source for the tricarboxylic acid cycle.
CC {ECO:0000255|HAMAP-Rule:MF_01904}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01904};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01904};
CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_01904}.
CC -!- SIMILARITY: Belongs to the PEPCase type 2 family. {ECO:0000255|HAMAP-
CC Rule:MF_01904}.
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DR EMBL; CP000504; ABL87432.1; -; Genomic_DNA.
DR RefSeq; WP_011762009.1; NC_008701.1.
DR AlphaFoldDB; A1RR50; -.
DR SMR; A1RR50; -.
DR STRING; 384616.Pisl_0252; -.
DR EnsemblBacteria; ABL87432; ABL87432; Pisl_0252.
DR GeneID; 4616997; -.
DR KEGG; pis:Pisl_0252; -.
DR eggNOG; arCOG04435; Archaea.
DR HOGENOM; CLU_517433_0_0_2; -.
DR OMA; QSAFRYD; -.
DR OrthoDB; 23334at2157; -.
DR Proteomes; UP000002595; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR HAMAP; MF_01904; PEPcase_type2; 1.
DR InterPro; IPR007566; PEP_COase_arc-type.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR Pfam; PF14010; PEPcase_2; 1.
DR PIRSF; PIRSF006677; UCP006677; 1.
DR SUPFAM; SSF51621; SSF51621; 1.
DR TIGRFAMs; TIGR02751; PEPCase_arch; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation; Lyase; Magnesium.
FT CHAIN 1..461
FT /note="Phosphoenolpyruvate carboxylase"
FT /id="PRO_0000309610"
SQ SEQUENCE 461 AA; 52098 MW; 9BFEF067B5DB6709 CRC64;
MIPRLMCTQH PDATVKVTAN EEVDEALVAY TAYGCDEVMI DYEGKTTPYS QPKDITVKAY
EAGLPLGEKF FLTPRMPNPR LEEFERSMLT LEAAILANYF SVKLMGRQAI RWIVLPMVTD
IETLGLVYRM LLHKTEAYIK ETGVKLEPPE LIPLIEDAIA QLKADELISG LLKQMAQQPQ
YIRLFLGKSD SAVRHGHLAS ALAIVATLSK MRSVEKSLGI KIYPILGMGS PPFRGGINNP
SLSHLETIQY VGYYTVTVQS AVRYDTSYDE YIKVRETILN ACCLPSREIN LPELEEIITK
ASSTYKSVVV KFADRIVQMA RLIPGTRDRI SWTVYGRNIT AEDRVVNMPR AIVYTSAWYA
MGLPPTFLDA PTVVELAKSD KLDLVLKALP SLRREWEYDA QFYDPQVASR YTSEDFVKVV
NEMLDYLGIN LRANGTYLSL LRMNRNESNI LAMGKYRKFL G
