ID   CAPPA_THEPD             Reviewed;         464 AA.
AC   A1RZN3;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_01904};
DE            Short=PEPC {ECO:0000255|HAMAP-Rule:MF_01904};
DE            Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_01904};
DE            EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_01904};
GN   Name=ppcA {ECO:0000255|HAMAP-Rule:MF_01904}; OrderedLocusNames=Tpen_1265;
OS   Thermofilum pendens (strain DSM 2475 / Hrk 5).
OC   Archaea; Crenarchaeota; Thermoprotei; Thermofilales; Thermofilaceae;
OC   Thermofilum.
OX   NCBI_TaxID=368408;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 2475 / Hrk 5;
RX   PubMed=18263724; DOI=10.1128/jb.01949-07;
RA   Anderson I., Rodriguez J., Susanti D., Porat I., Reich C., Ulrich L.E.,
RA   Elkins J.G., Mavromatis K., Lykidis A., Kim E., Thompson L.S., Nolan M.,
RA   Land M., Copeland A., Lapidus A., Lucas S., Detter C., Zhulin I.B.,
RA   Olsen G.J., Whitman W., Mukhopadhyay B., Bristow J., Kyrpides N.;
RT   "Genome sequence of Thermofilum pendens reveals an exceptional loss of
RT   biosynthetic pathways without genome reduction.";
RL   J. Bacteriol. 190:2957-2965(2008).
CC   -!- FUNCTION: Catalyzes the irreversible beta-carboxylation of
CC       phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon
CC       dicarboxylic acid source for the tricarboxylic acid cycle.
CC       {ECO:0000255|HAMAP-Rule:MF_01904}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01904};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01904};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_01904}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 2 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01904}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000505; ABL78663.1; -; Genomic_DNA.
DR   RefSeq; WP_011752928.1; NC_008698.1.
DR   AlphaFoldDB; A1RZN3; -.
DR   SMR; A1RZN3; -.
DR   STRING; 368408.Tpen_1265; -.
DR   EnsemblBacteria; ABL78663; ABL78663; Tpen_1265.
DR   GeneID; 4600480; -.
DR   KEGG; tpe:Tpen_1265; -.
DR   eggNOG; arCOG04435; Archaea.
DR   HOGENOM; CLU_517433_0_0_2; -.
DR   OMA; QSAFRYD; -.
DR   OrthoDB; 23334at2157; -.
DR   Proteomes; UP000000641; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_01904; PEPcase_type2; 1.
DR   InterPro; IPR007566; PEP_COase_arc-type.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   Pfam; PF14010; PEPcase_2; 1.
DR   PIRSF; PIRSF006677; UCP006677; 1.
DR   SUPFAM; SSF51621; SSF51621; 1.
DR   TIGRFAMs; TIGR02751; PEPCase_arch; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation; Lyase; Magnesium; Reference proteome.
FT   CHAIN           1..464
FT                   /note="Phosphoenolpyruvate carboxylase"
FT                   /id="PRO_0000309617"
SQ   SEQUENCE   464 AA;  51243 MW;  174AF466FC739834 CRC64;
     METPRLMCTQ HPDSTVKVPV QEEVEEAVRS FLVYGCDEVM SDYEGKLTPY AQPKEIVVKA
     GELGVPVGEG FYVTVRAPNP RLEDFDRVDL ALEAAVLANY YSYKRLGVQA VRWVVLPMTD
     SAETVRLVQR LLARKTRVLC EEVGQPCEQA QLVPLLEDVD SLLRVREILR DLHSALAELG
     SDPGVLRVFL GKSDSALKAG HIASALSLLY ALGESAKAGE ELGLEVKPIL GGGSPPFRGG
     VNNPRLVGVE VQRYRGYSTV TVQSAVRYDA SFSEYQEVRS KLLGGAGGEP GDAGGRVAEL
     ARLAASMYRS LASKYLDFVN EYARSVPTTR DRVSWREYGR ALELEDKLFS APRAIVYTAA
     WYSLGVPPTF LDADFVLEAY RGDFLDEVLG YLPGLEEEWR YDAQFYLPRL AGERLGEELV
     KKVDEALDAM GLRPEPLEPY EKLARTAPAE LRALLLGKVR GFLG