ID   CAPP_ALIF1              Reviewed;         876 AA.
AC   Q5E2E3;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_00595};
DE            Short=PEPC {ECO:0000255|HAMAP-Rule:MF_00595};
DE            Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_00595};
DE            EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_00595};
GN   Name=ppc {ECO:0000255|HAMAP-Rule:MF_00595}; OrderedLocusNames=VF_2308;
OS   Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Aliivibrio.
OX   NCBI_TaxID=312309;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700601 / ES114;
RX   PubMed=15703294; DOI=10.1073/pnas.0409900102;
RA   Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R.,
RA   Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E.,
RA   Stevens A., Visick K., Whistler C., Greenberg E.P.;
RT   "Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with
RT   pathogenic congeners.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005).
CC   -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC       for the tricarboxylic acid cycle. {ECO:0000255|HAMAP-Rule:MF_00595}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00595}.
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DR   EMBL; CP000020; AAW86803.1; -; Genomic_DNA.
DR   RefSeq; WP_011262715.1; NC_006840.2.
DR   RefSeq; YP_205691.1; NC_006840.2.
DR   AlphaFoldDB; Q5E2E3; -.
DR   SMR; Q5E2E3; -.
DR   STRING; 312309.VF_2308; -.
DR   EnsemblBacteria; AAW86803; AAW86803; VF_2308.
DR   KEGG; vfi:VF_2308; -.
DR   PATRIC; fig|312309.11.peg.2346; -.
DR   eggNOG; COG2352; Bacteria.
DR   HOGENOM; CLU_006557_2_0_6; -.
DR   OMA; PWVFGWT; -.
DR   OrthoDB; 398146at2; -.
DR   Proteomes; UP000000537; Chromosome I.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PTHR30523; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; SSF51621; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation; Lyase; Magnesium; Reference proteome.
FT   CHAIN           1..876
FT                   /note="Phosphoenolpyruvate carboxylase"
FT                   /id="PRO_0000166647"
FT   ACT_SITE        138
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
FT   ACT_SITE        543
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
SQ   SEQUENCE   876 AA;  98895 MW;  6E8E703847FC9012 CRC64;
     MNEKYAALKS NVSMLGHLLG NTIRDAHGEE LLAKVETIRK LSKTARAGSD EDRNALIEEI
     KSLPDDQLTP VARAFSQFLN LTNMAEQYHT ISRHCEAHVC EPDAISTLFS KLSQSNVSKL
     DTAQAVRELN IELVLTAHPT EIARRTMINK LVKINECLSK LELGDISFSE RDKTERRLEQ
     LIAQAWHSDV IRQERPTPLD EAKWGFAVVE NSLWQGIPEF LREFDQRLEG HLGEGLPIDA
     RPVHMSSWMG GDRDGNPFVT HKITREVMLL SRWKAADLYL KDINELISEL SMVKCTDEVR
     ELAGDQHEPY RAILKQLRTL LGDTLESLDA QMKGELAPNK VILTDADQLW NPLYACYQSL
     HACGMGIIAD GSLLDTLRRV KAFGAHLVRL DIRQESTRHS DVLSELTRYL GIGDYDQWSE
     QDKISFLVNE LSSKRPLLPR KWEPSPEVQE VIDTCKVVAE QSKEALGSYV ISMARTASDV
     LAVHLLLQEA GCPFRMDVCP LFETLDDLNR SKEVMEQLFS IDWYRGFIQN HQMVMIGYSD
     SAKDAGVMSA GWAQYSAMEA LVEVCEKESI ELTLFHGRGG TIGRGGAPAH AALLSQPPKS
     LKGGLRVTEQ GEMIRFKLGL PEVAVNSFNL YASAILEANL LPPPEPKQEW RDLMEVLSEV
     SCEAYRNVVR GEKDFVPYFR AATPELELGK LPLGSRPAKR NPNGGVESLR AIPWIFSWSQ
     NRLVLPAWLG AGEAIQYSID KGHQALLEEM CREWPFFSTR LGMLEMVYTK CNPQMSEYYD
     QRLTDKSLWP LGERLRNQLQ ADIKAVLNVE NNDHLMERDP WGSESIRLRN IYVDPLNMLQ
     AELLFRTRQQ EETSPELEEA LMVTIAGIAA GMRNTG