ID   Y043_METJA              Reviewed;         785 AA.
AC   Q60348;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 118.
DE   RecName: Full=Uncharacterized protein MJ0043;
DE   Contains:
DE     RecName: Full=Mja hyp1 intein;
GN   OrderedLocusNames=MJ0043;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- PTM: This protein undergoes a protein self splicing that involves a
CC       post-translational excision of the intervening region (intein) followed
CC       by peptide ligation. {ECO:0000305}.
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DR   EMBL; L77117; AAB98023.1; -; Genomic_DNA.
DR   PIR; C64305; C64305.
DR   RefSeq; WP_010869534.1; NC_000909.1.
DR   AlphaFoldDB; Q60348; -.
DR   SMR; Q60348; -.
DR   STRING; 243232.MJ_0043; -.
DR   EnsemblBacteria; AAB98023; AAB98023; MJ_0043.
DR   GeneID; 1450881; -.
DR   KEGG; mja:MJ_0043; -.
DR   eggNOG; arCOG04881; Archaea.
DR   HOGENOM; CLU_357040_0_0_2; -.
DR   InParanoid; Q60348; -.
DR   OMA; LIGPAHC; -.
DR   OrthoDB; 29545at2157; -.
DR   PhylomeDB; Q60348; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR   GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR   Gene3D; 3.10.28.10; -; 1.
DR   InterPro; IPR005287; CHP00375.
DR   InterPro; IPR003586; Hint_dom_C.
DR   InterPro; IPR003587; Hint_dom_N.
DR   InterPro; IPR036844; Hint_dom_sf.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR006142; INTEIN.
DR   InterPro; IPR030934; Intein_C.
DR   InterPro; IPR004042; Intein_endonuc.
DR   InterPro; IPR006141; Intein_N.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   PRINTS; PR00379; INTEIN.
DR   SMART; SM00305; HintC; 1.
DR   SMART; SM00306; HintN; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF51294; SSF51294; 2.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR   TIGRFAMs; TIGR01445; intein_Nterm; 1.
DR   TIGRFAMs; TIGR00375; TIGR00375; 1.
DR   PROSITE; PS50818; INTEIN_C_TER; 1.
DR   PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR   PROSITE; PS50817; INTEIN_N_TER; 1.
PE   4: Predicted;
KW   Autocatalytic cleavage; Protein splicing; Reference proteome.
FT   CHAIN           1..128
FT                   /note="Uncharacterized protein MJ0043, 1st part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000013988"
FT   CHAIN           129..520
FT                   /note="Mja hyp1 intein"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000013989"
FT   CHAIN           521..785
FT                   /note="Uncharacterized protein MJ0043, 2nd part"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000013990"
FT   DOMAIN          293..421
FT                   /note="DOD-type homing endonuclease"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00273"
SQ   SEQUENCE   785 AA;  91459 MW;  4167A59032511F17 CRC64;
     MIANVDLHIH SRFSGGTSKD MNVENILKYG KLKGLNIIGT GDCTHPDYLE EIKQYKDREL
     ILTTEIEDKN RVHHLILLPS ISKVEELREI LKKYSKDIDK EGRPRVSIGG AELLEIVRDV
     GGLIGPAHCV PPDTLLILEN GFKRIVDIKV GDKVLTHENR FKKVEKVYKR RYIGDIIKIK
     VRYFPEEIIL TPEHPVYAIK TEKRCDGSHG ICKFNCLTQY TNPSCKKRYR KYKREWIIAK
     DLKVGDVIVY PIPNRVRDIK YLSLDKYLSN IKREFCRSRI PEKIEVSEEF CRLVGYFLSE
     GYCFRDGIGF ALGENEKKII DDIEYLMKKI FNLKPKIRDD GRSEGIELKY YSRVLRDFFG
     DMFYCGDEKR AWNKALPNEF LYLPKNKQLQ IFIGWWRGDK GVTTSEILMN QLRLISLRLG
     FIITFSKHVP KNPKIGDREV IKYHARWQGR VSILDEKIVD ELKNEDIKLP KKDVRYGWIK
     GNYLYAPIIR IGREYYDGFV YNLEVEDDSS YVTVSGTLHN CFTPWTSLYK SFDSIYDCYN
     KKPDFVELGL SADTDMADMI PELRDLPFLS NSDAHSYHPH RLGREFNQIE VDYIGGIEDN
     FEQIKKAIKH NKIIANYGLD PKLGKYHLTA CSKCHTRFKL EDAKKYNWKC PKCGGSIKKG
     VLSRVEELSD GKIEHPKFRP PYYKLIPLAE MISLTIGKGI FTKAVQSLWE EFIKKYGNEI
     EVLINADIDE LSKIHPKVAE TINLFRKGKI YIYPGGGGEY GKISFKPQKV EWYREEVTLD
     RWLKQ