Y0574_MYCTU
ID Y0574_MYCTU Reviewed; 380 AA.
AC P9WM79; L0T731; O53771; Q7D9L9;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 35.
DE RecName: Full=Probable polyglutamine synthesis accessory protein Rv0574c {ECO:0000305};
GN OrderedLocusNames=Rv0574c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP INDUCTION BY HYPOXIA.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=11416222; DOI=10.1073/pnas.121172498;
RA Sherman D.R., Voskuil M., Schnappinger D., Liao R., Harrell M.I.,
RA Schoolnik G.K.;
RT "Regulation of the Mycobacterium tuberculosis hypoxic response gene
RT encoding alpha -crystallin.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:7534-7539(2001).
RN [3]
RP INDUCTION BY NITRIC OXIDE (NO) AND BY HYPOXIA, AND DORMANCY REGULON.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=12953092; DOI=10.1084/jem.20030205;
RA Voskuil M.I., Schnappinger D., Visconti K.C., Harrell M.I., Dolganov G.M.,
RA Sherman D.R., Schoolnik G.K.;
RT "Inhibition of respiration by nitric oxide induces a Mycobacterium
RT tuberculosis dormancy program.";
RL J. Exp. Med. 198:705-713(2003).
RN [4]
RP INDUCTION BY CARBON MONOXIDE (CO).
RC STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX PubMed=18474359; DOI=10.1016/j.chom.2008.03.007;
RA Shiloh M.U., Manzanillo P., Cox J.S.;
RT "Mycobacterium tuberculosis senses host-derived carbon monoxide during
RT macrophage infection.";
RL Cell Host Microbe 3:323-330(2008).
RN [5]
RP INDUCTION BY CARBON MONOXIDE (CO), AND DORMANCY REGULON.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=18400743; DOI=10.1074/jbc.m802274200;
RA Kumar A., Deshane J.S., Crossman D.K., Bolisetty S., Yan B.S., Kramnik I.,
RA Agarwal A., Steyn A.J.;
RT "Heme oxygenase-1-derived carbon monoxide induces the Mycobacterium
RT tuberculosis dormancy regulon.";
RL J. Biol. Chem. 283:18032-18039(2008).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [7]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=H37Rv;
RX PubMed=25312955; DOI=10.1128/iai.02274-14;
RA Garg R., Tripathi D., Kant S., Chandra H., Bhatnagar R., Banerjee N.;
RT "The conserved hypothetical protein Rv0574c is required for cell wall
RT integrity, stress tolerance, and virulence of Mycobacterium tuberculosis.";
RL Infect. Immun. 83:120-129(2015).
CC -!- FUNCTION: Could be involved in the biosynthesis, transport or
CC localization of poly-alpha-L-glutamine (PLG), a cell wall component.
CC Contributes to stress tolerance and virulence.
CC {ECO:0000269|PubMed:25312955}.
CC -!- INDUCTION: A member of the dormancy regulon. Induced in response to
CC reduced oxygen tension (hypoxia), low levels of nitric oxide (NO),
CC carbon monoxide (CO) and carbon dioxide (CO(2)). Expression peaks in
CC the late log phase of growth. {ECO:0000269|PubMed:11416222,
CC ECO:0000269|PubMed:12953092, ECO:0000269|PubMed:18400743,
CC ECO:0000269|PubMed:18474359, ECO:0000269|PubMed:25312955}.
CC -!- DISRUPTION PHENOTYPE: Disruption mutant shows reduced PLG content in
CC the cell wall, increased sensitivity to chemical and mechanical agents,
CC reduction of biofilm formation and attenuated virulence.
CC {ECO:0000269|PubMed:25312955}.
CC -!- SIMILARITY: Belongs to the CapA family. {ECO:0000305}.
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DR EMBL; AL123456; CCP43312.1; -; Genomic_DNA.
DR PIR; E70933; E70933.
DR RefSeq; NP_215088.1; NC_000962.3.
DR RefSeq; WP_003403008.1; NZ_NVQJ01000036.1.
DR AlphaFoldDB; P9WM79; -.
DR STRING; 83332.Rv0574c; -.
DR PaxDb; P9WM79; -.
DR PRIDE; P9WM79; -.
DR GeneID; 887721; -.
DR KEGG; mtu:Rv0574c; -.
DR TubercuList; Rv0574c; -.
DR eggNOG; COG2843; Bacteria.
DR OMA; LYGCGDF; -.
DR PhylomeDB; P9WM79; -.
DR PHI-base; PHI:4898; -.
DR Proteomes; UP000001584; Chromosome.
DR CDD; cd07381; MPP_CapA; 1.
DR Gene3D; 3.60.21.10; -; 1.
DR InterPro; IPR019079; Capsule_synth_CapA.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR Pfam; PF09587; PGA_cap; 1.
DR SMART; SM00854; PGA_cap; 1.
DR SUPFAM; SSF56300; SSF56300; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Stress response.
FT CHAIN 1..380
FT /note="Probable polyglutamine synthesis accessory protein
FT Rv0574c"
FT /id="PRO_0000392686"
SQ SEQUENCE 380 AA; 41547 MW; 5197A2C71A981EF6 CRC64;
MAGNPDVVTV LLGGDVMLGR GVDQILPHPG KPQLRERYMR DATGYVRLAE RVNGRIPLPV
DWRWPWGEAL AVLENTATDV CLINLETTIT ADGEFADRKP VCYRMHPDNV PALTALRPHV
CALANNHILD FGYQGLTDTV AALAGAGIQS VGAGADLLAA RRSALVTVGH ERRVIVGSVA
AESSGVPESW AARRDRPGVW LIRDPAQRDV ADDVAAQVLA DKRPGDIAIV SMHWGSNWGY
ATAPGDVAFA HRLIDAGIDM VHGHSSHHPR PIEIYRGKPI LYGCGDVVDD YEGIGGHESF
RSELRLLYLT VTDPASGNLI SLQMLPLRVS RMRLQRASQT DTEWLRNTIE RISRRFGIRV
VTRPDNLLEV VPAANLTSKE