ID   CAPP_ERWT9              Reviewed;         883 AA.
AC   B2VGA2;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_00595};
DE            Short=PEPC {ECO:0000255|HAMAP-Rule:MF_00595};
DE            Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_00595};
DE            EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_00595};
GN   Name=ppc {ECO:0000255|HAMAP-Rule:MF_00595}; OrderedLocusNames=ETA_01330;
OS   Erwinia tasmaniensis (strain DSM 17950 / CFBP 7177 / CIP 109463 / NCPPB
OS   4357 / Et1/99).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Erwinia.
OX   NCBI_TaxID=465817;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 17950 / CFBP 7177 / CIP 109463 / NCPPB 4357 / Et1/99;
RX   PubMed=18462403; DOI=10.1111/j.1462-2920.2008.01639.x;
RA   Kube M., Migdoll A.M., Mueller I., Kuhl H., Beck A., Reinhardt R.,
RA   Geider K.;
RT   "The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic
RT   bacterium in the genus Erwinia.";
RL   Environ. Microbiol. 10:2211-2222(2008).
CC   -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC       for the tricarboxylic acid cycle. {ECO:0000255|HAMAP-Rule:MF_00595}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00595}.
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DR   EMBL; CU468135; CAO95179.1; -; Genomic_DNA.
DR   RefSeq; WP_012439903.1; NC_010694.1.
DR   AlphaFoldDB; B2VGA2; -.
DR   SMR; B2VGA2; -.
DR   STRING; 465817.ETA_01330; -.
DR   EnsemblBacteria; CAO95179; CAO95179; ETA_01330.
DR   KEGG; eta:ETA_01330; -.
DR   eggNOG; COG2352; Bacteria.
DR   HOGENOM; CLU_006557_2_0_6; -.
DR   OMA; PWVFGWT; -.
DR   OrthoDB; 398146at2; -.
DR   Proteomes; UP000001726; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PTHR30523; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; SSF51621; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation; Lyase; Magnesium; Reference proteome.
FT   CHAIN           1..883
FT                   /note="Phosphoenolpyruvate carboxylase"
FT                   /id="PRO_1000129833"
FT   ACT_SITE        138
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
FT   ACT_SITE        546
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
SQ   SEQUENCE   883 AA;  98644 MW;  E61CA1254C10C39D CRC64;
     MNEQYSAMRS NVSMLGKLLG DTIKDALGEN ILDRVETIRK LSKSSRAGND AHRQELLSTL
     QNLSNDELLP VARAFSQFLN LTNVAEQYHT ISPNGEGAKN PELLLQTFQR LKQQPNLTEA
     AVCEALGSLS LELVLTAHPT EITRRTLIHK LVEVNSCLKQ LDHNDLSDYE HAQIMRRLRQ
     LVAQAWHTDE IRKYRPSPVD EAKWGFAVVE NSLWEGVPQF LRELNEQVEA AFGYTLPVDF
     VPVQFTSWMG GDRDGNPNVT ADITRHVLQL SRWKATDLFL RDIAVLISEL SMSECTPEVR
     ELCGNPEALE PYREIMKNLR SQLMSTQAYL AGRLKGERLT RPANLLIANE QLWEPLYTCY
     QSLQACGMGI IANGQLLDTL RRVKCFGVPL VRIDIRQEST RHTEAIAEIT RYLGLGDYES
     WSEADKQAFL IRELNSKRPL TPRQWEPSAD TKEVLDTCRV AAEAPKGSIA AYVISMAKTP
     SDVLAVHLLL KEAGIDYAMP VAPLFETLDD LNNANGVMSQ LLNIDWYRGL IQGKQMVMIG
     YSDSAKDAGV MAASWAQYQA QDALIKTCEK AGITLTLFHG RGGSIGRGGA PAHAALLSQP
     PGSLKGGLRV TEQGEMIRFK YGLPEVTISS LSLYTGAILE ANLLPPPEPK TEWIGIMERL
     SAVSCKMYRG YVREHAEFVP YFRSATPEQE LAKLPLGSRP AKRRPTGGVE SLRAIPWIFA
     WTQNRLMLPA WLGAGAALQQ VMEDGHQDQL EAMCRDWPFF STRLGMLEMV FSKADLWLAE
     YYDQRLVDKS LWPLGKQLRD QLESDIKAVL TIANDAHLMA DQPWIAESIA LRNVYTDPLN
     VLQAELLHRS RQQEKAGGEP DARVEQALMV TIAGVAAGMR NTG