ID   Y0785_MYCTU             Reviewed;         566 AA.
AC   P71838; L0T7Q3;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 2.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=KsdD-like steroid dehydrogenase Rv0785;
DE            EC=1.3.99.-;
GN   OrderedLocusNames=Rv0785;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Able to catalyze the elimination of the C-1 and C-2 hydrogen
CC       atoms of the A-ring from the polycyclic ring structure of 3-
CC       ketosteroids. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- PATHWAY: Lipid metabolism; steroid biosynthesis.
CC   -!- SIMILARITY: Belongs to the FAD-dependent oxidoreductase 2 family.
CC       {ECO:0000305}.
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DR   EMBL; AL123456; CCP43532.1; -; Genomic_DNA.
DR   PIR; D70709; D70709.
DR   RefSeq; NP_215299.1; NC_000962.3.
DR   RefSeq; WP_003911278.1; NZ_NVQJ01000035.1.
DR   AlphaFoldDB; P71838; -.
DR   SMR; P71838; -.
DR   STRING; 83332.Rv0785; -.
DR   PaxDb; P71838; -.
DR   DNASU; 885864; -.
DR   GeneID; 885864; -.
DR   KEGG; mtu:Rv0785; -.
DR   PATRIC; fig|83332.111.peg.870; -.
DR   TubercuList; Rv0785; -.
DR   eggNOG; COG3573; Bacteria.
DR   InParanoid; P71838; -.
DR   OMA; PEDHWPR; -.
DR   PhylomeDB; P71838; -.
DR   UniPathway; UPA00062; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0033765; F:steroid dehydrogenase activity, acting on the CH-CH group of donors; IEA:UniProt.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.50.50.60; -; 1.
DR   Gene3D; 3.90.700.10; -; 1.
DR   InterPro; IPR003953; FAD-binding_2.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR027477; Succ_DH/fumarate_Rdtase_cat_sf.
DR   InterPro; IPR014614; UCP036654.
DR   PANTHER; PTHR43260; PTHR43260; 1.
DR   Pfam; PF00890; FAD_binding_2; 1.
DR   PIRSF; PIRSF036654; UCP036654; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   1: Evidence at protein level;
KW   FAD; Flavoprotein; Lipid degradation; Lipid metabolism; Oxidoreductase;
KW   Reference proteome; Steroid metabolism.
FT   CHAIN           1..566
FT                   /note="KsdD-like steroid dehydrogenase Rv0785"
FT                   /id="PRO_0000403955"
FT   BINDING         23..54
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   566 AA;  61252 MW;  366D371B83EB930F CRC64;
     MALTCTDMSD AVAGSDAEGL TADAIVVGAG LAGLVAACEL ADRGLRVLIL DQENRANVGG
     QAFWSFGGLF LVNSPEQRRL GIRDSHELAL QDWLGTAAFD RPEDYWPEQW AHAYVDFAAG
     EKRSWLRARG LKIFPLVGWA ERGGYDAQGH GNSVPRFHIT WGTGPALVDI FVRQLRDRPT
     VRFAHRHQVD KLIVEGNAVT GVRGTVLEPS DEPRGAPSSR KSVGKFEFRA SAVIVASGGI
     GGNHELVRKN WPRRMGRIPK QLLSGVPAHV DGRMIGIAQK AGAAVINPDR MWHYTEGITN
     YDPIWPRHGI RIIPGPSSLW LDAAGKRLPV PLFPGFDTLG TLEYITKSGH DYTWFVLNAK
     IIEKEFALSG QEQNPDLTGR RLGQLLRSRA HAGPPGPVQA FIDRGVDCVH ANSLRELVAA
     MNELPDVVPL DYETVAAAVT ARDREVVNKY SKDGQITAIR AARRYRGDRF GRVVAPHRLT
     DPKAGPLIAV KLHILTRKTL GGIETDLDAR VLKADGTPLA GLYAAGEVAG FGGGGVHGYR
     ALEGTFLGGC IFSGRAAGRG AAEDIR