ID   CAPP_LACAC              Reviewed;         912 AA.
AC   Q5FK38;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_00595};
DE            Short=PEPC {ECO:0000255|HAMAP-Rule:MF_00595};
DE            Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_00595};
DE            EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_00595};
GN   Name=ppc {ECO:0000255|HAMAP-Rule:MF_00595}; OrderedLocusNames=LBA1092;
OS   Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=272621;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700396 / NCK56 / N2 / NCFM;
RX   PubMed=15671160; DOI=10.1073/pnas.0409188102;
RA   Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L.,
RA   McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S.,
RA   Hamrick A., Cano R., Klaenhammer T.R.;
RT   "Complete genome sequence of the probiotic lactic acid bacterium
RT   Lactobacillus acidophilus NCFM.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005).
CC   -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC       for the tricarboxylic acid cycle. {ECO:0000255|HAMAP-Rule:MF_00595}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00595}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000033; AAV42936.1; -; Genomic_DNA.
DR   RefSeq; WP_011254335.1; NC_006814.3.
DR   RefSeq; YP_193967.1; NC_006814.3.
DR   AlphaFoldDB; Q5FK38; -.
DR   SMR; Q5FK38; -.
DR   STRING; 272621.LBA1092; -.
DR   PRIDE; Q5FK38; -.
DR   EnsemblBacteria; AAV42936; AAV42936; LBA1092.
DR   GeneID; 56942701; -.
DR   KEGG; lac:LBA1092; -.
DR   PATRIC; fig|272621.13.peg.1039; -.
DR   eggNOG; COG2352; Bacteria.
DR   HOGENOM; CLU_006557_2_0_9; -.
DR   OMA; PWVFGWT; -.
DR   BioCyc; LACI272621:G1G49-1087-MON; -.
DR   Proteomes; UP000006381; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PTHR30523; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; SSF51621; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation; Lyase; Magnesium; Reference proteome.
FT   CHAIN           1..912
FT                   /note="Phosphoenolpyruvate carboxylase"
FT                   /id="PRO_0000166598"
FT   ACT_SITE        138
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
FT   ACT_SITE        575
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
SQ   SEQUENCE   912 AA;  104353 MW;  EE482B5472CFC8D4 CRC64;
     MTFTKLENSS DQAVVAEEVK ILTKLLNEST RQLIGDDAFA KIQDLIDTSA SKDQKQLESK
     ISSLNNREMI VVARYFATLP LLINISEDVE LASKVNVFNN TDQDYLGKLS DTIDLVAQKD
     DAKRILENVN VVPVLTAHPT QIQRKTVLEL TDKIHHLLRS YRDVKNGTIN QREWTEQLRA
     CIEILMQTDI IRGHKLKVSN EITNVLAYYP KALIPAITKF TTRYKELAKE HNLTLDQATP
     ITMGMWIGGD RDGNPYVTAD TLELSATLQS QVIFEYYMKE LKKLYRAISL STSYMQPSAA
     VEKLSKLSND DSPFRTDEPY RRAFYYIESR LVHTEKELLG IIDKNIFIKP HDLENLDNIP
     VYNNPQEFKS DLETIKASLD EDHDQAVTHS FFTQILEAID VFGFHLATID MRQDSSVNES
     CVAELLKSAG ICDNYSDLSE KEKVELLLSE LENDPRNLHA NNKPKSELLQ KELKIYKTAR
     QLKDRLGEDV IKQHIISHTE SVSDMLEQAI MLKEYNLVDS EKARIQVVPL FETVEDLLNA
     REIITQYLSF PIVKKWLVSQ NNYQEIMLGY SDSNKDGGYL ASCWNLYKAQ KDLTAIGEKL
     GVKITYMHGR GGTVGRGGGP SYEAITAQPF KSINDRIRMT EQGEIIQNKY GNKDTAYYNL
     EMLASAAIDR MVSKQAVSEE RITDFRSSMD KIVEESNKIY RKLVFENPAF LDYFFQATPI
     KEISNLNIGS RPASRKKLTD FSGLRAIPWV FSWSQSRIMF PGWYGVGSAF ANFINADPTH
     LKELQEMYKG WPFFHSLLSN VDMFLSKSNM EIAREYASLC DDEETKKVFD IIYQEWKLTK
     KVILQIEDHD DLLAEAPILK QSLDYRMPYF NILNYIQIEM IKRGREDEIQ GVYQSIIPIT
     INGVASGLRN SG