CAPP_NITEC
ID CAPP_NITEC Reviewed; 933 AA.
AC Q0AH90;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000255|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000255|HAMAP-Rule:MF_00595}; OrderedLocusNames=Neut_1036;
OS Nitrosomonas eutropha (strain DSM 101675 / C91 / Nm57).
OC Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC Nitrosomonadaceae; Nitrosomonas.
OX NCBI_TaxID=335283;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 101675 / C91 / Nm57;
RX PubMed=17991028; DOI=10.1111/j.1462-2920.2007.01409.x;
RA Stein L.Y., Arp D.J., Berube P.M., Chain P.S., Hauser L., Jetten M.S.,
RA Klotz M.G., Larimer F.W., Norton J.M., Op den Camp H.J.M., Shin M., Wei X.;
RT "Whole-genome analysis of the ammonia-oxidizing bacterium, Nitrosomonas
RT eutropha C91: implications for niche adaptation.";
RL Environ. Microbiol. 9:2993-3007(2007).
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000255|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family. {ECO:0000255|HAMAP-
CC Rule:MF_00595}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000450; ABI59292.1; -; Genomic_DNA.
DR RefSeq; WP_011634115.1; NC_008344.1.
DR AlphaFoldDB; Q0AH90; -.
DR SMR; Q0AH90; -.
DR STRING; 335283.Neut_1036; -.
DR EnsemblBacteria; ABI59292; ABI59292; Neut_1036.
DR KEGG; net:Neut_1036; -.
DR eggNOG; COG2352; Bacteria.
DR HOGENOM; CLU_006557_2_0_4; -.
DR OMA; PWVFGWT; -.
DR OrthoDB; 398146at2; -.
DR Proteomes; UP000001966; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PTHR30523; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; SSF51621; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation; Lyase; Magnesium.
FT CHAIN 1..933
FT /note="Phosphoenolpyruvate carboxylase"
FT /id="PRO_1000025569"
FT ACT_SITE 158
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
FT ACT_SITE 592
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
SQ SEQUENCE 933 AA; 106506 MW; C37860FB1F4D9C87 CRC64;
MSLNTLANIV PEKDTSLEKD HPLREDIRLL GRMLGDTIRE LEGEPMFDLV ETIRQTAVRF
RREQDEEAGK ELDTILNHLS HKETTAVVRA FSYFSLLSNI AEDLHHNRRR RAHLRAGSPP
QDGSVTLALE RVVAKGINTQ QLENFFAEAL ISPVLTAHPT EVQRRSILDY QLKIQRLLKE
RDRTQLTPNE LRHNEEDLRS AIQTLWQTRV LRSVRLTVQD EIENGLIYYH YTFLKQIPYI
YAKIEDILER HMDKAAPCIA SFLRIGSWIG GDRDGNPFVT HQIMLHAAER HSALILDYYI
EEVEKIGQAM SLTERLIKVT SELEGLASTA PGLPASRIDE PYRRVFLGMH TRLIATSRYL
SPHVGRQYQE NTAEPYADSA EFVHDLDIVI RSLKQHQSNK LAQGAIRDLR RAVDVFGFHL
ASLDMRQHSK IHEQVVSELY EKNAQDNRNY PDLTRAERAE WLLAELKRSH PLVSSLSDYS
DITQGELRIL RMAAEIQRRF GHVALPNYII SMATGVIHIL EAALLLKEAG LLQFGEAARS
TVNIIPLFET IDDLRGCANV MEELFSLPEY RKMLRSRSNL QEVMLGYSDS NKDGGFVTSN
WEIHKAEIEL TKVFNRHGVR LRLFHGRGGT VGRGGGPSYQ GILAQPPGSV NGQIRLTEQG
EVIASKYTDP EIGRRNLETL VAATIESTLL DRDAIHCHTP QCYQIMEELS ASAYAAYRDL
VYKTPNFTQF FQESTPIREI AGLHIGSRPT SRKPSNKIED LRTIPWVFSW SLNRTMIPGW
YGFGTAVENF VQQAGNTQDV LIQMQKMYRT WPFFQTLLSN MDMVLAKSDL GIASRYAELV
TDSELRQYVF TAIRTEWELC VKWLFAITGH TELLQDNPTL ARSIRIRTPY IDPMNHLQVE
LLRRYRSGDD DDAIRRAIHL TINGVATGLR NSG