ID   CAPP_PARXL              Reviewed;         994 AA.
AC   Q143B6;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_00595};
DE            Short=PEPC {ECO:0000255|HAMAP-Rule:MF_00595};
DE            Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_00595};
DE            EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_00595};
GN   Name=ppc {ECO:0000255|HAMAP-Rule:MF_00595}; OrderedLocusNames=Bxeno_A1035;
GN   ORFNames=Bxe_A3412;
OS   Paraburkholderia xenovorans (strain LB400).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Paraburkholderia.
OX   NCBI_TaxID=266265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LB400;
RX   PubMed=17030797; DOI=10.1073/pnas.0606924103;
RA   Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L.,
RA   Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., Lao V.,
RA   Larimer F., LiPuma J.J., Mahenthiralingam E., Malfatti S.A., Marx C.J.,
RA   Parnell J.J., Ramette A., Richardson P., Seeger M., Smith D., Spilker T.,
RA   Sul W.J., Tsoi T.V., Ulrich L.E., Zhulin I.B., Tiedje J.M.;
RT   "Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome
RT   shaped for versatility.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15280-15287(2006).
CC   -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC       for the tricarboxylic acid cycle. {ECO:0000255|HAMAP-Rule:MF_00595}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00595}.
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DR   EMBL; CP000270; ABE29573.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q143B6; -.
DR   SMR; Q143B6; -.
DR   STRING; 266265.Bxe_A3412; -.
DR   EnsemblBacteria; ABE29573; ABE29573; Bxe_A3412.
DR   KEGG; bxe:Bxe_A3412; -.
DR   eggNOG; COG2352; Bacteria.
DR   OMA; PWVFGWT; -.
DR   Proteomes; UP000001817; Chromosome 1.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PTHR30523; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; SSF51621; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation; Lyase; Magnesium; Reference proteome.
FT   CHAIN           1..994
FT                   /note="Phosphoenolpyruvate carboxylase"
FT                   /id="PRO_1000025554"
FT   REGION          1..67
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        7..22
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        204
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
FT   ACT_SITE        646
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
SQ   SEQUENCE   994 AA;  109655 MW;  018ADC914C1D9199 CRC64;
     MKAVRSDKTT QAATTAQAQK PAKAGSSKIK IVTAAPQAAN ASARQPASAQ APAPKANGRT
     REDKDHPLFQ DIRYLGRLLG DVLREQEGDA VFDVVETIRQ TAVRFRREDD SAAAQTLDKK
     LRSLSPEQTV SVVRAFSYFS HLANIAEDRH RNRRHRIHEL AGSTSQPGTI AHSLERLVEA
     GAAATPVLQE FFNNALIVPV LTAHPTEVQR KSILDAQHDV ARLLAERDQQ LTDRERAHNE
     AMLRARVTSL WQTRMLRDSR LSVADEIENA LSYYRATFLE EIPALYADIE EALAEHGLEA
     RLPPFFQMGS WIGGDRDGNP NVTAETLENA ITRQAAVIFE HYMEQVHKLG AELSVSNLLA
     GASDALKELA AVSPDQSPHR TDEPYRRALI GMYTRLAASA RVRLGEGSVP VRSAGRGAAP
     VRAKPYADSA EFVRDLHVLI DSLAEHHGAP LAAPRLSPLA RAAEVFGFHL ASIDLRQSSD
     VHEAVITELL RRAGVEENYA ELPEADKLNV LLSELAQPRP LRLPFAEYSD LVKSELGVLE
     EARVTREKFG ARAVRNYIIS HTETVSDLVE VMLLQKETGL LRGCLGNAND PAQAGLMVIP
     LFETIPDLRN APHIMRDLIA LPGVDALIEH QGNEQEVMLG YSDSNKDGGF LTSNWELYRA
     ELALVSLFNE RGVTLRLFHG RGGTVGRGGG PTYQAILSQP PGTVDGQIRL TEQGEVIASK
     FGNPEIGRRN LETVVAATLE ASLLPHGIAP AQLPAFEETM QQLSDAAMAS YRALVYETPG
     FKEYFFESTP ISEIAELNIG SRPASRKLQD PKQRKIEDLR AIPWGFSWGQ CRLLLTGWYG
     FGSAVAAHLD SAPSDAERTR RLALLKKMHK TWPFFANLLS NMDMVLAKTD LAVASRYAAL
     VSDKKLRKHV FERIVAEWER TSKVLSEITG KSERLAENPL LARSIKNRFP YLDPLNHLQV
     ELLKRHRAGD TNARVRRGIH LSINGIAAGL RNTG