CAPP_PECAS
ID CAPP_PECAS Reviewed; 879 AA.
AC Q6DAR6;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000255|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000255|HAMAP-Rule:MF_00595}; OrderedLocusNames=ECA0187;
OS Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia
OS carotovora subsp. atroseptica).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Pectobacteriaceae; Pectobacterium.
OX NCBI_TaxID=218491;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SCRI 1043 / ATCC BAA-672;
RX PubMed=15263089; DOI=10.1073/pnas.0402424101;
RA Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J.,
RA Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K.,
RA Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J.,
RA Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Norbertczak H.,
RA Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S.,
RA Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K.;
RT "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora
RT subsp. atroseptica and characterization of virulence factors.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004).
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000255|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family. {ECO:0000255|HAMAP-
CC Rule:MF_00595}.
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DR EMBL; BX950851; CAG73106.1; -; Genomic_DNA.
DR RefSeq; WP_011091826.1; NC_004547.2.
DR AlphaFoldDB; Q6DAR6; -.
DR SMR; Q6DAR6; -.
DR STRING; 218491.ECA0187; -.
DR PRIDE; Q6DAR6; -.
DR EnsemblBacteria; CAG73106; CAG73106; ECA0187.
DR GeneID; 57207044; -.
DR KEGG; eca:ECA0187; -.
DR PATRIC; fig|218491.5.peg.186; -.
DR eggNOG; COG2352; Bacteria.
DR HOGENOM; CLU_006557_2_0_6; -.
DR OMA; PWVFGWT; -.
DR OrthoDB; 398146at2; -.
DR Proteomes; UP000007966; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PTHR30523; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; SSF51621; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation; Lyase; Magnesium; Reference proteome.
FT CHAIN 1..879
FT /note="Phosphoenolpyruvate carboxylase"
FT /id="PRO_0000166595"
FT ACT_SITE 138
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
FT ACT_SITE 546
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
SQ SEQUENCE 879 AA; 98496 MW; 4E0058B1203221BD CRC64;
MNEQYSAMRS NVSMLGTLLG DTIKEALGEN ILDKVETIRK LSKSSRAGNE KHRQELLTTL
QNLSNDELLP VARAFSQFLN LTNTAEQYHT ISPHGEAASN PAQLSSAFKR LKESKDLSER
DIRDAVESLS IELVLTAHPT EITRRTLIHK LVEVNTCLKQ LDHNDLADYE RNQVMRRLRQ
LIAQSWHTDE IRKIRPTPVD EAKWGFAVVE NSLWEGVPAF LRELDEQLEQ AFGYRLPVDA
VPVRFTSWMG GDRDGNPNVT AEVTRHVLLL SRWKAADLFL RDIQVLVSEL SMSECTPELL
ELAGGSEVQE PYRAIMKSLR SQLSCTLSYL EARLTGEERL PPKDLLITNE QLWEPLHACY
QSLKTCGMGI IADGSLLDTL RRVRCFGVPL VRIDVRQEST RHTDALAEIT RYLGLGDYES
WSESDKQAFL IRELSSKRPL LPRYWEPSAD TKEVLDTCRV IAKAPQGSIA AYVISMARTP
SDVLAVQLLL KEAGCPFALP VAPLFETLDD LNNADDVMTQ LLSIDWYRGF IQGKQMVMIG
YSDSAKDAGV MAASWAQYRA QDALIKTCEK AGIALTLFHG RGGSIGRGGA PAHAALLSQP
PGSLKGGLRV TEQGEMIRFK YGLPDVTISS LALYTGAILE ANLLPPPEPK QEWHEVMDEL
SRVSCDMYRG YVRENPDFVP YFRAATPELE LGKLPLGSRP AKRRPNGGVE SLRAIPWIFA
WTQNRLMLPA WLGAGAGLQK VVDDGKQEQL EEMCRNWPFF STRIGMLEMV FAKADLWLAE
YYDQRLVEEK LWPLGKQLRD QLAADINIVL AISNDDHLMA DLPWIAESIA LRNVYTDPLN
VLQAELLHRS RQQKQPDADL ELALMVTIAG VAAGMRNTG