ID   CAPP_PICAB              Reviewed;         963 AA.
AC   P51063;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Phosphoenolpyruvate carboxylase;
DE            Short=PEPC;
DE            Short=PEPCase;
DE            EC=4.1.1.31;
GN   Name=PPC;
OS   Picea abies (Norway spruce) (Picea excelsa).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Picea.
OX   NCBI_TaxID=3329;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Cotyledon, and Stem;
RA   Relle M., Sutter A., Wild A.;
RT   "A method to isolate cDNA-quality RNA from adult conifer needles and a psbA
RT   cDNA from Norway spruce.";
RL   J. Plant Physiol. 149:225-228(1996).
CC   -!- FUNCTION: Through the carboxylation of phosphoenolpyruvate (PEP) it
CC       forms oxaloacetate, a four-carbon dicarboxylic acid source for the
CC       tricarboxylic acid cycle.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- ACTIVITY REGULATION: By light-reversible phosphorylation.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family. {ECO:0000305}.
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DR   EMBL; X79090; CAA55700.1; -; mRNA.
DR   PIR; S49344; S49344.
DR   AlphaFoldDB; P51063; -.
DR   SMR; P51063; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PTHR30523; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; SSF51621; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   2: Evidence at transcript level;
KW   Allosteric enzyme; Carbon dioxide fixation; Cytoplasm; Lyase; Magnesium;
KW   Phosphoprotein; Photosynthesis.
FT   CHAIN           1..963
FT                   /note="Phosphoenolpyruvate carboxylase"
FT                   /id="PRO_0000166674"
FT   ACT_SITE        172
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        600
FT                   /evidence="ECO:0000250"
FT   MOD_RES         11
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   963 AA;  109552 MW;  DF9C63F77E179FD4 CRC64;
     MARNNLEKMA SIDAQMRLLV PGKVSEDDKL IEYDALLLDR FLDILQDLHG EDIRAMVQEC
     YERSGEYEGK NDPHKLEELG NVLTSLDPGD SIVVASSFSH MLNLANLAEE VQIAYRRRNK
     IKRGGFADES NATTESDIEE TFKRLVNQLG KSPAEVFDAL KNQTVDLVLT AHPTQSVRRS
     LLQKHARIRN CLSQLYGKDI TPDEKQELDE ALLREIQAAF RTDEIRRTPP TPQDEMRAGM
     SYFHETIWKG VPKFLRRIDT ALKSIGINER VPYNAPLIQF SSWMGGDRDG NPRVTPEVTR
     DVCLLARMMA ANLYYSQIED LMFELSMWRC SDELRARALQ LHSASKKDAK HYIEFWKQIP
     PNEPFRVILG DVRDKLYNTR ERTRQLLSNG ISDIPEEVTF TNIDEFLEPL ELCYRSLCST
     GDQPIADGSL LDFMRQVSTF GLSFVKLDIR QESDRHSDVA DAITRHLGIG SYKEWSEEQR
     QAWLLSELQG KRPLFGPDLP KTDEVRDVLD TFHVISELPA DNFGAYIISM ATAASDVLVV
     ELLQRECHVK KPLRVVPLFE KLADLEAAPA ALARLFSINW YRNRIDGKQE VMIGYSDSGK
     DAGRLSAGWA LYKAQEDLIK VAKEFGIKLT MFHGRGGTVG RGGGPTHLAI LSQPPDTIHG
     SFRVTVQGEV IEQSFGEEHL CFRTLQRFTA ATLEHGMRPP VAPKPEWREL MDEMAVVATK
     EYRSIVFQDP RFVEYFRSAT PELEYGRMNI GSRPSKRKPS GGIESLRAIP WIFAWTQTRF
     HLPVWLGFGA AFKHVMEKDI RNLHMLQQMY NEWPFFRVTI DLIEMVFAKG DPGIAALYDK
     LLVSDDLWAI GEKLRANYGE TKDLLLQVAG HKDLLEGDPY LKQRLRLRDS YITTLNVCQA
     YTLKRIRDPN YHVNLRPHLS KESSTKPAAE LVKLNPTSEY APGLEDTLIL TMKGIAAGMQ
     NTG