ID   Y1013_MYCTU             Reviewed;         544 AA.
AC   O05598; L0T866;
DT   10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 2.
DT   25-MAY-2022, entry version 117.
DE   RecName: Full=Putative ligase Rv1013;
DE            EC=6.2.1.-;
GN   Name=pks16; OrderedLocusNames=Rv1013;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   PUPYLATION AT LYS-528, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=20066036; DOI=10.1371/journal.pone.0008589;
RA   Festa R.A., McAllister F., Pearce M.J., Mintseris J., Burns K.E.,
RA   Gygi S.P., Darwin K.H.;
RT   "Prokaryotic ubiquitin-like protein (Pup) proteome of Mycobacterium
RT   tuberculosis.";
RL   PLoS ONE 5:E8589-E8589(2010).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- PTM: Pupylated at Lys-528 by the prokaryotic ubiquitin-like protein
CC       Pup, which probably leads to its degradation by the proteasome.
CC       {ECO:0000269|PubMed:20066036}.
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; AL123456; CCP43763.1; -; Genomic_DNA.
DR   PIR; H70603; H70603.
DR   RefSeq; NP_215529.1; NC_000962.3.
DR   RefSeq; WP_003405202.1; NZ_NVQJ01000018.1.
DR   AlphaFoldDB; O05598; -.
DR   SMR; O05598; -.
DR   STRING; 83332.Rv1013; -.
DR   PaxDb; O05598; -.
DR   DNASU; 886035; -.
DR   GeneID; 886035; -.
DR   KEGG; mtu:Rv1013; -.
DR   TubercuList; Rv1013; -.
DR   eggNOG; COG0318; Bacteria.
DR   InParanoid; O05598; -.
DR   OMA; AVESNAW; -.
DR   PhylomeDB; O05598; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0070566; F:adenylyltransferase activity; IBA:GO_Central.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IBA:GO_Central.
DR   CDD; cd05931; FAAL; 1.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.40.50.12780; -; 1.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR028154; AMP-dep_Lig_C.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   InterPro; IPR042099; ANL_N_sf.
DR   InterPro; IPR040097; FAAL/FAAC.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF14535; AMP-binding_C_2; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   1: Evidence at protein level;
KW   Isopeptide bond; Ligase; Reference proteome; Ubl conjugation.
FT   CHAIN           1..544
FT                   /note="Putative ligase Rv1013"
FT                   /id="PRO_0000395881"
FT   CROSSLNK        528
FT                   /note="Isoglutamyl lysine isopeptide (Lys-Gln) (interchain
FT                   with Q-Cter in protein Pup)"
FT                   /evidence="ECO:0000269|PubMed:20066036"
SQ   SEQUENCE   544 AA;  58467 MW;  B91D412C110EF246 CRC64;
     MSRFTEKMFH NARTATTGMV TGEPHMPVRH TWGEVHERAR CIAGGLAAAG VGLGDVVGVL
     AGFPVEIAPT AQALWMRGAS LTMLHQPTPR TDLAVWAEDT MTVIGMIEAK AVIVSEPFLV
     AIPILEQKGM QVLTVADLLA SDPIGPIEVG EDDLALMQLT SGSTGSPKAV QITHRNIYSN
     AEAMFVGAQY DVDKDVMVSW LPCFHDMGMV GFLTIPMFFG AELVKVTPMD FLRDTLLWAK
     LIDKYQGTMT AAPNFAYALL AKRLRRQAKP GDFDLSTLRF ALSGAEPVEP ADVEDLLDAG
     KPFGLRPSAI LPAYGMAETT LAVSFSECNA GLVVDEVDAD LLAALRRAVP ATKGNTRRLA
     TLGPLLQDLE ARIIDEQGDV MPARGVGVIE LRGESLTPGY LTMGGFIPAQ DEHGWYDTGD
     LGYLTEEGHV VVCGRVKDVI IMAGRNIYPT DIERAAGRVD GVRPGCAVAV RLDAGHSRES
     FAVAVESNAF EDPAEVRRIE HQVAHEVVAE VDVRPRNVVV LGPGTIPKTP SGKLRRANSV
     TLVT