ID   Y1024_STRP3             Reviewed;         462 AA.
AC   P0DG14; Q878W2; Q8K722;
DT   27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 1.
DT   03-AUG-2022, entry version 45.
DE   RecName: Full=Uncharacterized RNA methyltransferase SpyM3_1024;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=SpyM3_1024;
OS   Streptococcus pyogenes serotype M3 (strain ATCC BAA-595 / MGAS315).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=198466;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-595 / MGAS315;
RX   PubMed=12122206; DOI=10.1073/pnas.152298499;
RA   Beres S.B., Sylva G.L., Barbian K.D., Lei B., Hoff J.S., Mammarella N.D.,
RA   Liu M.-Y., Smoot J.C., Porcella S.F., Parkins L.D., Campbell D.S.,
RA   Smith T.M., McCormick J.K., Leung D.Y.M., Schlievert P.M., Musser J.M.;
RT   "Genome sequence of a serotype M3 strain of group A Streptococcus: phage-
RT   encoded toxins, the high-virulence phenotype, and clone emergence.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:10078-10083(2002).
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01024}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM79631.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE014074; AAM79631.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_011106727.1; NC_004070.1.
DR   AlphaFoldDB; P0DG14; -.
DR   SMR; P0DG14; -.
DR   EnsemblBacteria; AAM79631; AAM79631; SpyM3_1024.
DR   KEGG; spg:SpyM3_1024; -.
DR   HOGENOM; CLU_014689_7_2_9; -.
DR   OMA; FYAGDMK; -.
DR   Proteomes; UP000000564; Chromosome.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR030391; MeTrfase_TrmA_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   PANTHER; PTHR11061; PTHR11061; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
DR   PROSITE; PS01231; TRMA_2; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..462
FT                   /note="Uncharacterized RNA methyltransferase SpyM3_1024"
FT                   /id="PRO_0000162037"
FT   DOMAIN          12..70
FT                   /note="TRAM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00208"
FT   ACT_SITE        419
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         294
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         323
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         344
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         392
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   462 AA;  51751 MW;  242E2A4867AF0205 CRC64;
     MVSPRKGKRI RMLKKNDIIQ VAISDLSHEG AGVAKHDGFV FFVDNALPEE VIDMRVLKVN
     KNSGFGKVEA YHYLSPARNA DVNLTYLRTG IADLGHLTYE DQLTFKKKQV QDSLYKIAGI
     SDVTVESTIG MTEPLAYRNK AQVPVRRVNG QLETGFFRKH SHDLIPISDY YIQDKEIDRL
     INFTRDLLRR FDIKPYDETE QTGLLRNIVV RRGHYSGEMM LVLVTTRPKV FRVDQVIEKI
     VEAFPAVVSI IQNINDKNTN AIFGKDFKTL YGKDTITDSM LGNNYAISAQ SFYQVNTVMA
     EKLYQTAIAF SDLSKDDIVI DAYSGIGTIG LSFAKTVKAV YGVEVIEAAV RDAQHNAALN
     GITNAYFVAD TAEHAMATWA KDGIKPSVIL VDPPRKGLTE SFIQASVAMG PQKITYVSCN
     PATMARDIKR YQELGYKLAK VQPVDLFPQT HHVECVVLLI KE