ID   CAPP_PSEF5              Reviewed;         876 AA.
AC   Q4KHK5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_00595};
DE            Short=PEPC {ECO:0000255|HAMAP-Rule:MF_00595};
DE            Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_00595};
DE            EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_00595};
GN   Name=ppc {ECO:0000255|HAMAP-Rule:MF_00595}; OrderedLocusNames=PFL_1147;
OS   Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=220664;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-477 / NRRL B-23932 / Pf-5;
RX   PubMed=15980861; DOI=10.1038/nbt1110;
RA   Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A.,
RA   Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J.,
RA   Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J.,
RA   Gwinn M.L., Zhou L., Schneider D.J., Cartinhour S.W., Nelson W.C.,
RA   Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III,
RA   Thomashow L.S., Loper J.E.;
RT   "Complete genome sequence of the plant commensal Pseudomonas fluorescens
RT   Pf-5.";
RL   Nat. Biotechnol. 23:873-878(2005).
CC   -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC       for the tricarboxylic acid cycle. {ECO:0000255|HAMAP-Rule:MF_00595}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00595}.
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DR   EMBL; CP000076; AAY90434.1; -; Genomic_DNA.
DR   RefSeq; WP_011059495.1; NC_004129.6.
DR   AlphaFoldDB; Q4KHK5; -.
DR   SMR; Q4KHK5; -.
DR   STRING; 220664.PFL_1147; -.
DR   EnsemblBacteria; AAY90434; AAY90434; PFL_1147.
DR   KEGG; pfl:PFL_1147; -.
DR   PATRIC; fig|220664.5.peg.1178; -.
DR   eggNOG; COG2352; Bacteria.
DR   HOGENOM; CLU_006557_2_0_6; -.
DR   OMA; PWVFGWT; -.
DR   OrthoDB; 398146at2; -.
DR   Proteomes; UP000008540; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PTHR30523; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; SSF51621; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation; Lyase; Magnesium; Reference proteome.
FT   CHAIN           1..876
FT                   /note="Phosphoenolpyruvate carboxylase"
FT                   /id="PRO_1000025577"
FT   ACT_SITE        138
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
FT   ACT_SITE        543
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
SQ   SEQUENCE   876 AA;  97194 MW;  D99E4D3AC5ED7ED0 CRC64;
     MTDIDARLRE DVHLLGELLG NTIRDQYGEA FLDKIEQIRK GAKADRRGSP GAELSASLDQ
     LSEDELLPVA RAFNQFLNLA NIAEQYQLIH RRDESQPAPF EARVLPELLA RLRAEGHSAD
     ALARQLGRLE IELVLTAHPT EVARRTLIQK YDAIAGQLAA QDHRDLTSAE RGQIQERLQR
     LIAEAWHTEE IRRTRPTPVD EAKWGFAVIE HSLWQAIPNY LRKADKALFE ATGLHLPLES
     APIRFASWMG GDRDGNPNVT AAVTREVLLL ARWMAADLYL RDIDHLAAEL SMQQASPALL
     ARAGDSAEPY RALLKQLRER LRATRNWAHA ALSAAVPAGA EVLHNNRDLL EPLQLCYQSL
     HECGMGVIAD GPLLDCLRRA VTFGLFLVRL DVRQDSSRHT SAMTEITDYL GLGRYEDWSE
     EDRITFLMRE LSSRRPLLPG YFKPSADTAE VLATCREIAG APAASLGSYV ISMAGAASDV
     LAVQLLLKES GVLRPMRVVP LFETLADLDN AGPVMEKLLH MPGYRSRLQG PQEVMIGYSD
     SAKDAGTTAA AWAQYRAQER LVDICREQQV ELLLFHGRGG TVGRGGGPAH AAILSQPPGS
     VAGRFRTTEQ GEMIRFKFGL PDIAEQNLNL YLAAVLEATL LPPPPPQPAW RHLMDELASD
     GVRAYREVVR DNPQFVEYFR QSTPEQELGR LPLGSRPAKR RAGGIESLRA IPWIFGWTQT
     RLMLPAWLGW EAALSKALAR GEGDLLGQMR EQWPFFRTRI DMLEMVLAKA DADIARSYDE
     RLVEAELLPL GEHLRDLLSQ ACAVVLGLTG QSQLLAHSPD TLEFIRLRNT YLDPLHLLQA
     ELLARSRKQQ APQGSPVEQA LLVSVAGIAA GLRNTG