CAPP_PSEPG
ID CAPP_PSEPG Reviewed; 875 AA.
AC B0KS63;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 1.
DT 25-MAY-2022, entry version 72.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000255|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000255|HAMAP-Rule:MF_00595}; OrderedLocusNames=PputGB1_1110;
OS Pseudomonas putida (strain GB-1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=76869;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GB-1;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Bruce D., Goodwin L., Chertkov O., Brettin T.,
RA Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Kim E., McCarthy J.K., Richardson P.;
RT "Complete sequence of Pseudomonas putida GB-1.";
RL Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000255|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family. {ECO:0000255|HAMAP-
CC Rule:MF_00595}.
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DR EMBL; CP000926; ABY97018.1; -; Genomic_DNA.
DR RefSeq; WP_012270799.1; NC_010322.1.
DR AlphaFoldDB; B0KS63; -.
DR SMR; B0KS63; -.
DR STRING; 76869.PputGB1_1110; -.
DR EnsemblBacteria; ABY97018; ABY97018; PputGB1_1110.
DR KEGG; ppg:PputGB1_1110; -.
DR eggNOG; COG2352; Bacteria.
DR HOGENOM; CLU_006557_2_0_6; -.
DR OMA; PWVFGWT; -.
DR Proteomes; UP000002157; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PTHR30523; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; SSF51621; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation; Lyase; Magnesium.
FT CHAIN 1..875
FT /note="Phosphoenolpyruvate carboxylase"
FT /id="PRO_1000082430"
FT ACT_SITE 137
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
FT ACT_SITE 542
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
SQ SEQUENCE 875 AA; 96876 MW; F575014741808C5C CRC64;
MTDIDVRLRE DVHVLGELLG ETIRQQHGDA FLQKIEDIRH SAKADRRGPG EQLSSTLADL
AEDDLLPVAR AFNQFLNLAN MAEQYQLIRR RDADQPEPFE ARVLPELLAR LKQAGHSNDA
LARQLAKLDI QLVLTAHPTE VARRTLIQKY DAIAGQLAAQ DHRDLSPAER QQVRERLRRL
IAEAWHTEEI RRTRPTPVDE AKWGFAVIEH SLWHAIPSHL RKVDKALLEA TGLRLPLEAA
PIRFASWMGG DRDGNPNVTA AVTREVLLLA RWMAADLFLR DIDALAAELS MQQANDALRE
RVGDSAEPYR AVLKQLRDRL RATRAWAHSA LASNQPAGAE VLVDNRDLIA PLELCYQSLH
DCGMGVIAEG PLLDCLRRAV TFGLFLGRLD VRQDAARHRD ALTEITDYLG LGRYADWDEE
QRIGFLQAEL KNRRPLLPAH FKPQADTAEV LATCREVAAA PAASLGSYVI SMAGAASDVL
AVQLLLKEAG LTRPMRVVPL FETLADLDNA GPVMQRLLGL PGYRAGLRGP QEVMIGYSDS
AKDAGTTAAA WAQYRAQENL VRICAEHQVE LLLFHGRGGT VGRGGGPAHA AILSQPPGSV
AGRFRTTEQG EMIRFKFGLP GIAEQNLNLY LAAVLEATLL PPPPPQPAWR ELMDQLAADG
VKAYRSVVRE NPDFVEYFRQ STPEQELGRL PLGSRPAKRR AGGIESLRAI PWIFGWTQTR
LMLPAWLGWE TALSNALARG QGELLAQMRE QWPFFRTRID MLEMVLAKAD AQIAEAYDER
LVQPHLLPLG AHLRDLLSQS CQVVLGLTGQ PVLLAHSPET LEFISLRNTY LDPLHRLQAE
LLARSRSREA ALDSPLEQAL LVTVAGIAAG LRNTG