ID   CAPP_PSEU5              Reviewed;         879 AA.
AC   A4VN44;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2007, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_00595};
DE            Short=PEPC {ECO:0000255|HAMAP-Rule:MF_00595};
DE            Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_00595};
DE            EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_00595};
GN   Name=ppc {ECO:0000255|HAMAP-Rule:MF_00595}; OrderedLocusNames=PST_2745;
OS   Pseudomonas stutzeri (strain A1501).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=379731;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=A1501;
RX   PubMed=18495935; DOI=10.1073/pnas.0801093105;
RA   Yan Y., Yang J., Dou Y., Chen M., Ping S., Peng J., Lu W., Zhang W.,
RA   Yao Z., Li H., Liu W., He S., Geng L., Zhang X., Yang F., Yu H., Zhan Y.,
RA   Li D., Lin Z., Wang Y., Elmerich C., Lin M., Jin Q.;
RT   "Nitrogen fixation island and rhizosphere competence traits in the genome
RT   of root-associated Pseudomonas stutzeri A1501.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:7564-7569(2008).
CC   -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC       for the tricarboxylic acid cycle. {ECO:0000255|HAMAP-Rule:MF_00595}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00595}.
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DR   EMBL; CP000304; ABP80395.1; -; Genomic_DNA.
DR   RefSeq; WP_011913852.1; NC_009434.1.
DR   AlphaFoldDB; A4VN44; -.
DR   SMR; A4VN44; -.
DR   STRING; 379731.PST_2745; -.
DR   EnsemblBacteria; ABP80395; ABP80395; PST_2745.
DR   KEGG; psa:PST_2745; -.
DR   eggNOG; COG2352; Bacteria.
DR   HOGENOM; CLU_006557_2_0_6; -.
DR   OMA; PWVFGWT; -.
DR   Proteomes; UP000000233; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PTHR30523; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; SSF51621; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation; Lyase; Magnesium; Reference proteome.
FT   CHAIN           1..879
FT                   /note="Phosphoenolpyruvate carboxylase"
FT                   /id="PRO_1000072624"
FT   ACT_SITE        141
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
FT   ACT_SITE        546
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
SQ   SEQUENCE   879 AA;  97821 MW;  06C443CBFB4FFB90 CRC64;
     MAKIIDARLR DNVHLLGELL GNTIRAQHGD QFFDKIERIR KGAKAARKGS AEGAQLLAET
     LDSLDESELL PMTRAFNQFL NLANIAEQYH QVRRRAAGEP APFEIGVFAD LIERLKAAGH
     DNEFIARQVS RLEIELVLTA HPTEVSRRTL IQKYDAIAQQ LAARDHTDLS DAERASIELS
     LQRLIAEVWH TEEIRRNRPT PVEEAKWGFA AIENSLWKAV PNVLRQTDAT LHRLTGLHLP
     LEAAPIRFAS WMGGDRDGNP NVTAMVSREV LLTARWVAAD LYLREIEGLI TALSMREASD
     ELLRQSGDSA EPYRVLLKPL RQRLRATREW ARAAIEHGQP APAEVLQDCA ELRRPLELCY
     RSLHACGMGV IADGALLDCL RRLAVFGLFL VRLDIRQDAA RHAAALAEIT DYLGLGDYQQ
     WDEQKRLDWL QHELANRRPL LPAHYHPSAD TAEVLATCAV IAEAPAASLG SYVISMAHAA
     SDVLAVQLLL KEAGLQRPMR VVPLFETLDD LNNAAPTIDR LLSLPGYRQR LHGPQEVMIG
     YSDSAKDAGT TAAAWAQYRA QEQLVEVCRE HGVELLLFHG RGGTVGRGGG PAHAAILSQP
     PGSVAGRFRT TEQGEMIRFK FGLPDIAEQN LNLYLAAVLE ATLLPPPPPE PGWRETMDRL
     AAEGVAAYRG VVREHPLFVD YFRQATPELE LGRLPLGSRP AKRREGGVES LRAIPWIFAW
     TQTRLMLPAW LGWEQALHGA LQRGEGERLA EMRARWPFFS TRIDMLEMVL AKADADIARR
     YDERLVQPEL LSLGSDLRDR LSQVIEAVLS LTGQSDLLDH SPKTQEAFSL RNTYLDPLHL
     MQIELLARSR QRQGPAESPL EQALLVSVAG IAAGLRNTG