CAPP_RALSO
ID CAPP_RALSO Reviewed; 985 AA.
AC Q8XWW2;
DT 08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000255|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000255|HAMAP-Rule:MF_00595}; OrderedLocusNames=RSc2358;
GN ORFNames=RS01188;
OS Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Ralstonia.
OX NCBI_TaxID=267608;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GMI1000;
RX PubMed=11823852; DOI=10.1038/415497a;
RA Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M.,
RA Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M.,
RA Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M.,
RA Moisan A., Robert C., Saurin W., Schiex T., Siguier P., Thebault P.,
RA Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A.;
RT "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL Nature 415:497-502(2002).
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000255|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family. {ECO:0000255|HAMAP-
CC Rule:MF_00595}.
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DR EMBL; AL646052; CAD16065.1; -; Genomic_DNA.
DR RefSeq; WP_011002281.1; NC_003295.1.
DR AlphaFoldDB; Q8XWW2; -.
DR SMR; Q8XWW2; -.
DR STRING; 267608.RSc2358; -.
DR EnsemblBacteria; CAD16065; CAD16065; RSc2358.
DR GeneID; 60501867; -.
DR KEGG; rso:RSc2358; -.
DR PATRIC; fig|267608.8.peg.2397; -.
DR eggNOG; COG2352; Bacteria.
DR HOGENOM; CLU_006557_2_0_4; -.
DR OMA; PWVFGWT; -.
DR Proteomes; UP000001436; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PTHR30523; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; SSF51621; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation; Lyase; Magnesium; Reference proteome.
FT CHAIN 1..985
FT /note="Phosphoenolpyruvate carboxylase"
FT /id="PRO_0000166616"
FT REGION 1..55
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 29..51
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 193
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
FT ACT_SITE 634
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
SQ SEQUENCE 985 AA; 108869 MW; B6F7B42856505B1D CRC64;
MTQSAARRAS SRATPARKTP PAPASQTPAP SPGGTAGTAL GPTSRRSSGS AAAKDQPLKE
DIRFLGRLLG DVLREQEGAA AFETVETIRQ TAVRFRRDGD RQAEQELDRL LKTLSRDQTT
SVVRAFSYFS HLANIAEDQH HNRRRRVHAL AGSSPQPGSL LRALLSAADE GLSGDALRRF
FDAALIVPVL TAHPTEVQRK SILDAQREIA RLLAERDAPL TVRERERNVT LLRAHVTKLW
QTRMLRTTRL MVADEIENAL SYYQTTFLRE IPALYRELEE DVATVFPRRG ARGEPAPLPA
FFQMGSWIGG DRDGNPFVTA QTLRHAAQRQ ASVILTWYLD EIHALGAELS MSTLLVDVSA
DLLALAERSP DHSEHRADEP YRRALIGVYA RLAATCRELT GEDAGRHAVG PAPAYTRAEE
LRADLQIVID SLAAHHGEAL ADARLASLAR AIDVFGFHLA SIDLRQVSDV HEATVAELLR
VAGVEGAYAA LSEADKRTLL LRELQQPRLL TLPFHTYSET TASELDIFRA AREVRARYGS
RIVRNYIISH TETLSDLLEV MLLQKEAGMF RHGTNGSGGA GLDVMVIPLF ETIEDLRNAP
QIMGELLALP GFDAVLAAQG NEQEVMLGYS DSNKDGGFLT SNWELYKTEL ALVELFERKG
VRLRLFHGRG GTVGRGGGPT YQAILSQPPG TVNGQIRLTE QGEIISSKFA NPEIGRRNLE
TIVAATLEAT LLPTRNRPKG LEEFEAAMQA LSDHAFSAYR HLVYETPGFK DYFFATTPIT
EIADLNLGSR PASRKLMDRK QRRIEDLRAI PWGFSWGQCR LLLPGWFGFG SAVQRWLDEA
GSAKAKAARL ATLKRMHKQW PFFANLLSNM DMVLSKADLN VASRYAQLCE DRKLRNAVFS
RISAEFTLTE QVLGAITGQS ERLADNPLLA RSIKNRFPYL DPLNHLQVEL LKRFRSGKAG
SNDARVRRGI HLSINGIAAG LRNSG