ID   Y1070_STRP6             Reviewed;         462 AA.
AC   Q5XBK8;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Uncharacterized RNA methyltransferase M6_Spy1070;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=M6_Spy1070;
OS   Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=286636;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-946 / MGAS10394;
RX   PubMed=15272401; DOI=10.1086/422697;
RA   Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E.,
RA   Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M.;
RT   "Progress toward characterization of the group A Streptococcus metagenome:
RT   complete genome sequence of a macrolide-resistant serotype M6 strain.";
RL   J. Infect. Dis. 190:727-738(2004).
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01024}.
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DR   EMBL; CP000003; AAT87205.1; -; Genomic_DNA.
DR   RefSeq; WP_011106727.1; NC_006086.1.
DR   AlphaFoldDB; Q5XBK8; -.
DR   SMR; Q5XBK8; -.
DR   EnsemblBacteria; AAT87205; AAT87205; M6_Spy1070.
DR   KEGG; spa:M6_Spy1070; -.
DR   HOGENOM; CLU_014689_7_0_9; -.
DR   OMA; FYAGDMK; -.
DR   Proteomes; UP000001167; Chromosome.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR030391; MeTrfase_TrmA_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   PANTHER; PTHR11061; PTHR11061; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
DR   PROSITE; PS01231; TRMA_2; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..462
FT                   /note="Uncharacterized RNA methyltransferase M6_Spy1070"
FT                   /id="PRO_0000162039"
FT   DOMAIN          12..70
FT                   /note="TRAM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00208"
FT   ACT_SITE        419
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         294
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         323
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         344
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         392
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   462 AA;  51751 MW;  242E2A4867AF0205 CRC64;
     MVSPRKGKRI RMLKKNDIIQ VAISDLSHEG AGVAKHDGFV FFVDNALPEE VIDMRVLKVN
     KNSGFGKVEA YHYLSPARNA DVNLTYLRTG IADLGHLTYE DQLTFKKKQV QDSLYKIAGI
     SDVTVESTIG MTEPLAYRNK AQVPVRRVNG QLETGFFRKH SHDLIPISDY YIQDKEIDRL
     INFTRDLLRR FDIKPYDETE QTGLLRNIVV RRGHYSGEMM LVLVTTRPKV FRVDQVIEKI
     VEAFPAVVSI IQNINDKNTN AIFGKDFKTL YGKDTITDSM LGNNYAISAQ SFYQVNTVMA
     EKLYQTAIAF SDLSKDDIVI DAYSGIGTIG LSFAKTVKAV YGVEVIEAAV RDAQHNAALN
     GITNAYFVAD TAEHAMATWA KDGIKPSVIL VDPPRKGLTE SFIQASVAMG PQKITYVSCN
     PATMARDIKR YQELGYKLAK VQPVDLFPQT HHVECVVLLI KE