ID   CAPP_TOBAC              Reviewed;         964 AA.
AC   P27154;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Phosphoenolpyruvate carboxylase;
DE            Short=PEPC;
DE            Short=PEPCase;
DE            EC=4.1.1.31;
GN   Name=PPC;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Samsun NN;
RX   PubMed=1884006; DOI=10.1007/bf00040652;
RA   Koizumi N., Sato F., Terano Y., Yamada Y.;
RT   "Sequence analysis of cDNA encoding phosphoenolpyruvate carboxylase from
RT   cultured tobacco cells.";
RL   Plant Mol. Biol. 17:535-539(1991).
RN   [2]
RP   MISCELLANEOUS.
RX   PubMed=11807559; DOI=10.1038/415451a;
RA   Hibberd J.M., Quick W.P.;
RT   "Characteristics of C4 photosynthesis in stems and petioles of C3 flowering
RT   plants.";
RL   Nature 415:451-454(2002).
CC   -!- FUNCTION: Through the carboxylation of phosphoenolpyruvate (PEP) it
CC       forms oxaloacetate, a four-carbon dicarboxylic acid source for the
CC       tricarboxylic acid cycle. {ECO:0000250|UniProtKB:Q9MAH0}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:Q9MAH0};
CC   -!- ACTIVITY REGULATION: By light-reversible phosphorylation.
CC       {ECO:0000250}.
CC   -!- PATHWAY: Photosynthesis; C4 acid pathway.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:Q9MAH0}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- MISCELLANEOUS: Tobacco, a typical C3 plant, shows characteristics of C4
CC       photosynthesis in cells of stems and petioles that surround the xylem
CC       and phloem. {ECO:0000269|PubMed:11807559}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X59016; CAA41758.1; -; mRNA.
DR   PIR; S17440; QYNT.
DR   RefSeq; NP_001312166.1; NM_001325237.1.
DR   AlphaFoldDB; P27154; -.
DR   SMR; P27154; -.
DR   STRING; 4097.P27154; -.
DR   PRIDE; P27154; -.
DR   ProMEX; P27154; -.
DR   GeneID; 107777405; -.
DR   KEGG; nta:107777405; -.
DR   BRENDA; 4.1.1.31; 3645.
DR   UniPathway; UPA00322; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IBA:GO_Central.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-KW.
DR   GO; GO:0048366; P:leaf development; IBA:GO_Central.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PTHR30523; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; SSF51621; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   2: Evidence at transcript level;
KW   Allosteric enzyme; Carbon dioxide fixation; Cytoplasm; Lyase; Magnesium;
KW   Phosphoprotein; Photosynthesis; Reference proteome.
FT   CHAIN           1..964
FT                   /note="Phosphoenolpyruvate carboxylase"
FT                   /id="PRO_0000166682"
FT   ACT_SITE        172
FT                   /evidence="ECO:0000250|UniProtKB:P00864"
FT   ACT_SITE        600
FT                   /evidence="ECO:0000250|UniProtKB:P00864"
FT   MOD_RES         11
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9MAH0"
SQ   SEQUENCE   964 AA;  110164 MW;  5590F35694B22DE4 CRC64;
     MATRSLEKLA SIDAQLRALV PGKVSEDDKL VEYDALLLDR FLDILQDLHG EDLKETVQEC
     YELSAEYEGK HDPKKLEELG NVLTSLDPGD SIVIAKAFSH MLNLANLAEE VQIAYRRRQK
     LKRGDFADEN NATTESDIEE TFKKLVGDLK KSPQEVFDAL KNQTVDLVLT AHPTQSVRRS
     LLQKHGRIRD CLAQLYAKDI TPDDKQELDE ALQREIQAAF RTDEIRRTAP TPQDEMRAGM
     SYFHETIWKG VPKFLRRVDT ALKNIGINER LPYNAPLIQF SSWMGGDRDG NPRVTLEVTR
     DVCLLARMMA ANLYYSQIEE LMFELSMWRC NDDLRIRAAE LYRSSRRDTK HYIEFWKTIP
     PSEPYRVILG DVRDKLYQTR ERTRQMLAHG ISDIPEDATY NNVEQFLEPL ELCYRSLCEC
     GDRPIADGSL LDFLRQVSTF GLSFVRLDIR QESDRHTDVL DAITQHLEIG SYREWSEERR
     QEWLLSELSG KRPLFGPDLP RTEEIADVLD TLHVIAELPS DCFGAYIISM ATAPSDVLAV
     ELLQRECHVK QPLRVVPLFE KLDDLESASA AVARLFSIEW YRNRINGKQE VMVGYSDSGK
     DAGRFSAAWQ LYKAQEELIK VAKEHGVKLT MFHGRGGTVG RGGGPTHLAI LSQPPDTIQG
     SLRVTVQGEV IEQSFGEEHL CFRTLQRFTA ATLEHGMHPP VSPKPEWRAL MDEIAVIATE
     KYRSIVFKEP RFVEYSALAT PELEYGRMNI GSRPSKRKPS GGIESLRAIP WIFAWTQTRF
     HLPVWLGFGA AFKYAIDKDI KNLRMFHEMY NEWPFFRVTI DLVEMVFAKG NPGIAALYDK
     LLVSEDLLPF GELLRSNYEE TRSLLLQIAG HKDLLEGDPY LKQRLRLRDS YITTLNLLQA
     YTLKRIRDPN YHVTLRPHIS KDYMESKSAA ELVQLNPTSE YAPGLEDTLI LTMKGIAAGL
     QNTG