ID   Y1151_LACPL             Reviewed;         457 AA.
AC   Q88XP4; F9UMV2;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Uncharacterized RNA methyltransferase lp_1151;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=lp_1151;
OS   Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
OS   (Lactobacillus plantarum).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactiplantibacillus.
OX   NCBI_TaxID=220668;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX   PubMed=12566566; DOI=10.1073/pnas.0337704100;
RA   Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P.,
RA   Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J.,
RA   Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M.,
RA   Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M.,
RA   Siezen R.J.;
RT   "Complete genome sequence of Lactobacillus plantarum WCFS1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX   PubMed=22156394; DOI=10.1128/jb.06275-11;
RA   Siezen R.J., Francke C., Renckens B., Boekhorst J., Wels M.,
RA   Kleerebezem M., van Hijum S.A.;
RT   "Complete resequencing and reannotation of the Lactobacillus plantarum
RT   WCFS1 genome.";
RL   J. Bacteriol. 194:195-196(2012).
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01024}.
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DR   EMBL; AL935263; CCC78541.1; -; Genomic_DNA.
DR   RefSeq; WP_011101269.1; NC_004567.2.
DR   RefSeq; YP_004889055.1; NC_004567.2.
DR   AlphaFoldDB; Q88XP4; -.
DR   SMR; Q88XP4; -.
DR   STRING; 220668.lp_1151; -.
DR   EnsemblBacteria; CCC78541; CCC78541; lp_1151.
DR   GeneID; 66449040; -.
DR   KEGG; lpl:lp_1151; -.
DR   PATRIC; fig|220668.9.peg.972; -.
DR   eggNOG; COG2265; Bacteria.
DR   HOGENOM; CLU_014689_7_0_9; -.
DR   OMA; FYAGDMK; -.
DR   PhylomeDB; Q88XP4; -.
DR   BioCyc; LPLA220668:G1GW0-983-MON; -.
DR   Proteomes; UP000000432; Chromosome.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR030391; MeTrfase_TrmA_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   PANTHER; PTHR11061; PTHR11061; 1.
DR   Pfam; PF01938; TRAM; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
DR   PROSITE; PS01231; TRMA_2; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..457
FT                   /note="Uncharacterized RNA methyltransferase lp_1151"
FT                   /id="PRO_0000161989"
FT   DOMAIN          6..64
FT                   /note="TRAM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00208"
FT   ACT_SITE        412
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         287
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         316
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         337
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         385
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   457 AA;  51003 MW;  B5EDC8A80F7ED2F8 CRC64;
     MKVNLPVHKG EVLDVTIMDL TYQGMGVAKV DNYPIFIENA LPEEKITVKV TKTTKNFAFG
     DVEKINQVSP HRVNPKGRVY RQTGIAPLQH LEYSEQLKFK QHQVAELFAK VHMDDVEVLP
     TIGMANPTQY RNKAQVPVRQ VQGQLTTGFY KKNSHQLMPI EDYYIQDPAI DKAIVVVRDI
     LRKYHEAAYD EFHHSGTIRT IMVRRGYYSH EMMVVIVTRT KHLPMADVVT QEIQAALPEV
     VSVIQNVNSK KTNVILGPVN NVLAGKATID DQLLGLTFAI SAQSFYQVNP QQTEKLYQLA
     IDQAGLTGNE TVIDAYSGIG TISLTMAQHA KQVYGVEIVP AAIDNARQNA DKNGITNATF
     VLDSAEKAMA KWQADGVKPD VIVVDPPRKG LDADFIKSAG EMAPKRVVYI SCNPSTLVRD
     VQRFAEYGYH ISAPVQPVDQ FPQTPHIESV TVLEREQ