ID   CAPS1_AFV1Y             Reviewed;         140 AA.
AC   Q70LC6;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   23-FEB-2022, entry version 42.
DE   RecName: Full=Major capsid protein 1 {ECO:0000303|PubMed:19934032};
DE   AltName: Full=MCP1;
GN   ORFNames=ORF140;
OS   Acidianus filamentous virus 1 (isolate United States/Yellowstone) (AFV-1).
OC   Viruses; Adnaviria; Zilligvirae; Taleaviricota; Tokiviricetes;
OC   Ligamenvirales; Lipothrixviridae; Gammalipothrixvirus.
OX   NCBI_TaxID=654909;
OH   NCBI_TaxID=563177; Acidianus hospitalis.
OH   NCBI_TaxID=12915; Acidianus infernus.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=14592760; DOI=10.1016/s0042-6822(03)00481-1;
RA   Bettstetter M., Peng X., Garrett R.A., Prangishvili D.;
RT   "AFV1, a novel virus infecting hyperthermophilic archaea of the genus
RT   acidianus.";
RL   Virology 315:68-79(2003).
RN   [2]
RP   SUBUNIT, AND FUNCTION.
RX   PubMed=32759221; DOI=10.1073/pnas.2011125117;
RA   Wang F., Baquero D.P., Beltran L.C., Su Z., Osinski T., Zheng W.,
RA   Prangishvili D., Krupovic M., Egelman E.H.;
RT   "Structures of filamentous viruses infecting hyperthermophilic archaea
RT   explain DNA stabilization in extreme environments.";
RL   Proc. Natl. Acad. Sci. U.S.A. 117:19643-19652(2020).
RN   [3] {ECO:0007744|PDB:3FBZ}
RP   X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 2-140, SUBCELLULAR LOCATION,
RP   FUNCTION, AND SUBUNIT.
RX   PubMed=19934032; DOI=10.1073/pnas.0909893106;
RA   Goulet A., Blangy S., Redder P., Prangishvili D., Felisberto-Rodrigues C.,
RA   Forterre P., Campanacci V., Cambillau C.;
RT   "Acidianus filamentous virus 1 coat proteins display a helical fold
RT   spanning the filamentous archaeal viruses lineage.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:21155-21160(2009).
RN   [4] {ECO:0007744|PDB:5W7G}
RP   STRUCTURE BY ELECTRON MICROSCOPY (4.50 ANGSTROMS).
RX   PubMed=28639939; DOI=10.7554/elife.26268;
RA   Kasson P., DiMaio F., Yu X., Lucas-Staat S., Krupovic M., Schouten S.,
RA   Prangishvili D., Egelman E.H.;
RT   "Model for a novel membrane envelope in a filamentous hyperthermophilic
RT   virus.";
RL   Elife 6:0-0(2017).
CC   -!- FUNCTION: Self-assembles to form a helical, filamentous nucleocapsid
CC       mesuring 900 nm in length and 24 nm in width (Probable)
CC       (PubMed:19934032). Together with capsid protein 2, wraps arounds the
CC       DNA and maintains it in an A-form (Probable). Capsid proteins probably
CC       maintain the DNA in A-form by non-specific desolvation and specific
CC       coordination of the DNA phosphate groups by positively charged residues
CC       (Probable). This certainly protects the viral DNA under conditions such
CC       as the extreme desiccation of its host (Probable).
CC       {ECO:0000269|PubMed:19934032, ECO:0000305|PubMed:32759221}.
CC   -!- SUBUNIT: Heterodimer composed of major capsid protein 1 and major
CC       capsid protein 2. {ECO:0000269|PubMed:19934032,
CC       ECO:0000269|PubMed:32759221}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:19934032}.
CC   -!- DOMAIN: The N-terminus projects into a DNA groove.
CC       {ECO:0000250|UniProtKB:Q914J4}.
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DR   EMBL; AJ567472; CAD98954.1; -; Genomic_DNA.
DR   RefSeq; YP_003750.1; NC_005830.1.
DR   PDB; 3FBZ; X-ray; 2.30 A; A/B/C/D=2-140.
DR   PDB; 5W7G; EM; 4.50 A; A/C/E/G/I/K/M/O/Q/S/U/W/Y/a/c/e/g/i/k/m/o=1-140.
DR   PDBsum; 3FBZ; -.
DR   PDBsum; 5W7G; -.
DR   SMR; Q70LC6; -.
DR   GeneID; 2769161; -.
DR   KEGG; vg:2769161; -.
DR   EvolutionaryTrace; Q70LC6; -.
DR   Proteomes; UP000000514; Genome.
DR   GO; GO:0019029; C:helical viral capsid; IDA:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Reference proteome; Virion.
FT   CHAIN           1..140
FT                   /note="Major capsid protein 1"
FT                   /id="PRO_0000384558"
FT   HELIX           17..20
FT                   /evidence="ECO:0007829|PDB:3FBZ"
FT   HELIX           21..23
FT                   /evidence="ECO:0007829|PDB:3FBZ"
FT   HELIX           32..66
FT                   /evidence="ECO:0007829|PDB:3FBZ"
FT   HELIX           71..73
FT                   /evidence="ECO:0007829|PDB:3FBZ"
FT   HELIX           74..94
FT                   /evidence="ECO:0007829|PDB:3FBZ"
FT   HELIX           98..117
FT                   /evidence="ECO:0007829|PDB:3FBZ"
FT   HELIX           121..136
FT                   /evidence="ECO:0007829|PDB:3FBZ"
SQ   SEQUENCE   140 AA;  15815 MW;  68A63096E4E7FC69 CRC64;
     MAKLNRKLRQ DSTDRYKTKL YLWRNLGGLI PEDMAISVTE SITADWKQYN DMMSKVRNET
     LDILKTNKVA TEDYIGYIAF AEELAHQVWK NKNSSPDPNT ANEASKTDLE SKYSDVYGLD
     VTVLDAIYNA VIPIIMGGGS