Y1178_ARATH
ID Y1178_ARATH Reviewed; 326 AA.
AC Q9XHZ8; Q0WS66;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 25-MAY-2022, entry version 136.
DE RecName: Full=BTB/POZ domain-containing protein At1g21780;
GN OrderedLocusNames=At1g21780; ORFNames=F8K7.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 60-326.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP DOMAIN BTB, AND INTERACTION WITH CUL3A AND CUL3B.
RX PubMed=15749712; DOI=10.1074/jbc.m413247200;
RA Gingerich D.J., Gagne J.M., Salter D.W., Hellmann H., Estelle M., Ma L.,
RA Vierstra R.D.;
RT "Cullins 3a and 3b assemble with members of the broad
RT complex/tramtrack/bric-a-brac (BTB) protein family to form essential
RT ubiquitin-protein ligases (E3s) in Arabidopsis.";
RL J. Biol. Chem. 280:18810-18821(2005).
RN [7]
RP INTERACTION WITH CUL3A, HOMODIMERIZATION, AND MUTAGENESIS OF ILE-204 AND
RP TYR-242.
RX PubMed=15772280; DOI=10.1105/tpc.105.031989;
RA Figueroa P., Gusmaroli G., Serino G., Habashi J., Ma L., Shen Y., Feng S.,
RA Bostick M., Callis J., Hellmann H., Deng X.W.;
RT "Arabidopsis has two redundant Cullin3 proteins that are essential for
RT embryo development and that interact with RBX1 and BTB proteins to form
RT multisubunit E3 ubiquitin ligase complexes in vivo.";
RL Plant Cell 17:1180-1195(2005).
CC -!- FUNCTION: May act as a substrate-specific adapter of an E3 ubiquitin-
CC protein ligase complex (CUL3-RBX1-BTB) which mediates the
CC ubiquitination and subsequent proteasomal degradation of target
CC proteins.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Homodimer. Interacts with CUL3A and CUL3B.
CC {ECO:0000269|PubMed:15749712, ECO:0000269|PubMed:15772280}.
CC -!- INTERACTION:
CC Q9XHZ8; Q9ZVH4: CUL3A; NbExp=4; IntAct=EBI-541047, EBI-531362;
CC -!- DOMAIN: The BTB/POZ domain mediates the interaction with some component
CC of ubiquitin ligase complexes. {ECO:0000269|PubMed:15749712}.
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DR EMBL; AC007727; AAD41432.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30155.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30156.1; -; Genomic_DNA.
DR EMBL; BT024719; ABD59057.1; -; mRNA.
DR EMBL; AK228073; BAF00033.1; -; mRNA.
DR EMBL; AY087548; AAM65090.1; -; mRNA.
DR PIR; E86351; E86351.
DR RefSeq; NP_001077574.1; NM_001084105.1.
DR RefSeq; NP_173597.1; NM_102027.3.
DR AlphaFoldDB; Q9XHZ8; -.
DR SMR; Q9XHZ8; -.
DR BioGRID; 24021; 4.
DR IntAct; Q9XHZ8; 3.
DR STRING; 3702.AT1G21780.2; -.
DR PaxDb; Q9XHZ8; -.
DR PRIDE; Q9XHZ8; -.
DR ProteomicsDB; 242519; -.
DR DNASU; 838782; -.
DR EnsemblPlants; AT1G21780.1; AT1G21780.1; AT1G21780.
DR EnsemblPlants; AT1G21780.2; AT1G21780.2; AT1G21780.
DR GeneID; 838782; -.
DR Gramene; AT1G21780.1; AT1G21780.1; AT1G21780.
DR Gramene; AT1G21780.2; AT1G21780.2; AT1G21780.
DR KEGG; ath:AT1G21780; -.
DR Araport; AT1G21780; -.
DR TAIR; locus:2036962; AT1G21780.
DR eggNOG; KOG1987; Eukaryota.
DR HOGENOM; CLU_061287_0_0_1; -.
DR InParanoid; Q9XHZ8; -.
DR OMA; ACSIWPN; -.
DR OrthoDB; 864323at2759; -.
DR PhylomeDB; Q9XHZ8; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q9XHZ8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9XHZ8; baseline and differential.
DR Genevisible; Q9XHZ8; AT.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR044714; AtSIBP1-like.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR46672; PTHR46672; 1.
DR Pfam; PF00651; BTB; 1.
DR SMART; SM00225; BTB; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..326
FT /note="BTB/POZ domain-containing protein At1g21780"
FT /id="PRO_0000406090"
FT DOMAIN 161..228
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT MUTAGEN 204
FT /note="I->R: Abolishes the interaction with CUL3A."
FT /evidence="ECO:0000269|PubMed:15772280"
FT MUTAGEN 242
FT /note="Y->A: Abolishes the interaction with CUL3A."
FT /evidence="ECO:0000269|PubMed:15772280"
SQ SEQUENCE 326 AA; 37678 MW; 34E3DB7AB972B25E CRC64;
MGDTKVETIS RLAQWRIENF GPCSFKKSDP FKVGIWNWHL SIERNRYLSV RLFPELSRVS
KEQPPVAKFV LRVSNVGPNR RFYISPVYEK LLRTTDDCVW HVDSSFHGRF TIDVEFLDLK
ICPVNGGEAS PVWPTDATMQ SISTQTTLKC LSRMLEESIL TDVIIHTADG TLSAHKAILS
ASSTVFKSMF HHDLMEKESS TIHIDDMSRE SCMALLSYLY GNITQEEFWK HRLALLGAAN
KYDITDLKAA CEESLMEDIN SSNVLERLQE AWLYQLEKLK KGCLMYLFDF GKIYDVREEI
SSFFRQADRE LMLEMFQEVL SVWKPV
