ID   CAPS2_AFV1Y             Reviewed;         132 AA.
AC   Q70LC7;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   23-FEB-2022, entry version 42.
DE   RecName: Full=Major capsid protein 2 {ECO:0000303|PubMed:19934032};
DE   AltName: Full=MCP2;
GN   ORFNames=ORF132;
OS   Acidianus filamentous virus 1 (isolate United States/Yellowstone) (AFV-1).
OC   Viruses; Adnaviria; Zilligvirae; Taleaviricota; Tokiviricetes;
OC   Ligamenvirales; Lipothrixviridae; Gammalipothrixvirus.
OX   NCBI_TaxID=654909;
OH   NCBI_TaxID=563177; Acidianus hospitalis.
OH   NCBI_TaxID=12915; Acidianus infernus.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=14592760; DOI=10.1016/s0042-6822(03)00481-1;
RA   Bettstetter M., Peng X., Garrett R.A., Prangishvili D.;
RT   "AFV1, a novel virus infecting hyperthermophilic archaea of the genus
RT   acidianus.";
RL   Virology 315:68-79(2003).
RN   [2]
RP   SUBUNIT, AND FUNCTION.
RX   PubMed=32759221; DOI=10.1073/pnas.2011125117;
RA   Wang F., Baquero D.P., Beltran L.C., Su Z., Osinski T., Zheng W.,
RA   Prangishvili D., Krupovic M., Egelman E.H.;
RT   "Structures of filamentous viruses infecting hyperthermophilic archaea
RT   explain DNA stabilization in extreme environments.";
RL   Proc. Natl. Acad. Sci. U.S.A. 117:19643-19652(2020).
RN   [3] {ECO:0007744|PDB:3FBL}
RP   X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 51-132, SUBCELLULAR LOCATION,
RP   FUNCTION, AND SUBUNIT.
RX   PubMed=19934032; DOI=10.1073/pnas.0909893106;
RA   Goulet A., Blangy S., Redder P., Prangishvili D., Felisberto-Rodrigues C.,
RA   Forterre P., Campanacci V., Cambillau C.;
RT   "Acidianus filamentous virus 1 coat proteins display a helical fold
RT   spanning the filamentous archaeal viruses lineage.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:21155-21160(2009).
RN   [4] {ECO:0007744|PDB:5W7G}
RP   STRUCTURE BY ELECTRON MICROSCOPY (4.50 ANGSTROMS).
RX   PubMed=28639939; DOI=10.7554/elife.26268;
RA   Kasson P., DiMaio F., Yu X., Lucas-Staat S., Krupovic M., Schouten S.,
RA   Prangishvili D., Egelman E.H.;
RT   "Model for a novel membrane envelope in a filamentous hyperthermophilic
RT   virus.";
RL   Elife 6:0-0(2017).
CC   -!- FUNCTION: Self-assembles to form a helical, filamentous nucleocapsid
CC       mesuring 900 nm in length and 24 nm in width (PubMed:19934032)
CC       (Probable). Together with capsid protein 1, wraps arounds the DNA and
CC       maintains it in an A-form (Probable). Capsid proteins probably maintain
CC       the DNA in A-form by non-specific desolvation and specific coordination
CC       of the DNA phosphate groups by positively charged residues (Probable).
CC       This certainly protects the viral DNA under conditions such as the
CC       extreme desiccation of its host (Probable).
CC       {ECO:0000269|PubMed:19934032, ECO:0000305|PubMed:32759221}.
CC   -!- SUBUNIT: Heterodimer composed of major capsid protein 1 and major
CC       capsid protein 2. {ECO:0000269|PubMed:19934032,
CC       ECO:0000269|PubMed:32759221}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:19934032}.
CC   -!- DOMAIN: The N-terminus projects into a DNA groove.
CC       {ECO:0000250|UniProtKB:Q914J5}.
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DR   EMBL; AJ567472; CAD98953.1; -; Genomic_DNA.
DR   RefSeq; YP_003749.1; NC_005830.1.
DR   PDB; 3FBL; X-ray; 1.95 A; A=51-132.
DR   PDB; 5W7G; EM; 4.50 A; B/D/F/H/J/L/N/P/R/T/V/X/Z/b/d/f/h/j/l/n/p=1-132.
DR   PDBsum; 3FBL; -.
DR   PDBsum; 5W7G; -.
DR   SMR; Q70LC7; -.
DR   GeneID; 2769168; -.
DR   KEGG; vg:2769168; -.
DR   EvolutionaryTrace; Q70LC7; -.
DR   Proteomes; UP000000514; Genome.
DR   GO; GO:0019029; C:helical viral capsid; IDA:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Reference proteome; Virion.
FT   CHAIN           1..132
FT                   /note="Major capsid protein 2"
FT                   /id="PRO_0000384555"
FT   HELIX           52..64
FT                   /evidence="ECO:0007829|PDB:3FBL"
FT   HELIX           69..71
FT                   /evidence="ECO:0007829|PDB:3FBL"
FT   HELIX           72..91
FT                   /evidence="ECO:0007829|PDB:3FBL"
FT   HELIX           96..111
FT                   /evidence="ECO:0007829|PDB:3FBL"
FT   HELIX           118..131
FT                   /evidence="ECO:0007829|PDB:3FBL"
SQ   SEQUENCE   132 AA;  14998 MW;  B89017A5CEF1E044 CRC64;
     MVNKKYRQDS KDRYQYKQYI YRSIGGIVPP EMAETVTANQ TAQWEAGFTP YHKLRLAIKE
     ICKTDGIPNI KWGMYIAFGE KLLKSYLKMK AGSASSDMIA EYINNAISAF SSRTGISQET
     AQKIADFITS NY